Industrial production process for wood frog body protein oligopeptide

A production process and oligopeptide technology, which is applied in the field of industrialized production process of wood frog body protein oligopeptide, can solve the problems of ignoring the development and utilization of wood frog body by-products, waste of resources, etc., achieve good decolorization effect and improve utilization rate , the effect of easy access to raw materials

Inactive Publication Date: 2018-06-19
通化百泉保健食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for a long time, people have focused on the production of high-quality Rana oil, while ignoring the development and utilization of Rana body by-products
Rana bodies are treated as waste or feed, resulting in a great waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A process for the industrialized production of Rana body protein oligopeptides, the steps of which are as follows:

[0049] 1. Raw materials

[0050] The raw material is the frog oil taken from the living body, the residual wood frog body after removing the skin, viscera and eggs, and the sun-dried wood frog body without corruption.

[0051] 2. Soak and soften

[0052] Put the dried wood frog body into a stainless steel cleaning tank, inject pure water, the water level should be 10-15cm higher than the wood frog body, stir evenly, soak for 48h-72h, change the water 2-4 times during the soaking, until the wood frog body Fully rehydrated to a fluffy state.

[0053] 3. Minced

[0054] After the soaked soft wood frog body is pulled out and drenched in water, it is put in a meat grinder and ground, and it is ground into fragments with a particle size of 0.1-0.2cm.

[0055] 4. Degreasing

[0056] Soak the minced Rana body in 1.0-2.0% acetic acid solution degreasing solut...

Embodiment 2

[0083] Weigh 100kg of dry wood frog body without corruption and soak for 48h-72h until the deer penis is rehydrated and becomes soft. Put it in a meat grinder and grind it into pieces with a particle size of 0.1-0.2cm. Degrease with 1.0-2.0% acetic acid solution for 1-2 days, rinse twice with warm water at 30-50°C. Grind with a bone mud mill to form a finely ground slurry with a particle size of 20-50 μm. Add 3-5% activated carbon by liquid weight to decolorize. Adjust the pH value to 4.0 to 4.5, add NaCl powder for salting out, press filter with a plate and frame filter press, add pepsin with a slurry weight of 0.1-2% to the slurry obtained after the filter press, and adjust the pH to pH 2 to 3. Under the temperature condition of 40-45°C, make the enzymatic hydrolysis reaction for 72 hours. Then add papain at 0.5% to 1% by weight of the slurry, adjust the pH to pH 6-8 at 40-45° C., and perform enzymatic hydrolysis for 5-10 hours to obtain a mixed solution containing oligop...

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Abstract

The invention provides an industrial production process for wood frog body protein oligopeptide. The industrial production process comprises the following specific steps: (1) raw material preparation,(2) soaking and softening, (3) trituration, (4) degreasing, (5) defibrination, (6) decoloration, (7) salting out, (8) filter pressing, (9) enzymolysis, (10) oligopeptide ultrafiltration, (11) filterpressing, entrapment, concentration, desalination and impurity removal; (12) degerming, (13) encapsulation, (14) freeze drying and (15) bottle cap plug pressing and labeling. The industrial productionprocess is simple and reasonable in design, natural resources are adequately utilized, and industrial and large-scale production can be realized. By hydrolyzing by virtue of food grade protease, thesafety is high, and side effects are avoided; and the wood frog body protein oligopeptide can be directly eaten as a health product, a functional food additive or a nutritional food and simultaneouslycan be safely used as a cosmetic additive.

Description

technical field [0001] The invention relates to an industrialized production process of a wood frog body protein oligopeptide, which belongs to the field of functional health products or health food and cosmetic additives. Background technique [0002] Due to the maturity and improvement of artificial breeding technology in recent years, the resources of Rana in Changbai Mountain are very rich. As a high-value medicinal animal, Rana changbai is widely used in food, medicine, cosmetics, health care products and so on. With the in-depth research on Rana, a variety of Rana products have been developed through modern high-tech means, and their application functionality has also been greatly improved. However, for a long time, people have focused on the production of high-quality Rana oil, while ignoring the development and utilization of Rana body by-products. Rana bodies are treated as waste or feed, which causes a great waste of resources. The limbs and shells of wood frogs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/30
CPCC07K14/78C12P21/06
Inventor 康杰任跃英骆长林康岩华正杰
Owner 通化百泉保健食品有限公司
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