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Application of silibinin in preparation of drugs for preventing and treating intestinal cancer correlated with colitis

A technology for silibinin and colitis, which is applied to the application field of silibinin in the preparation of drugs for preventing and treating colitis-related bowel cancer, can solve the problems of unsatisfactory curative effect and safety, and achieves reduction of inflammation recurrence and improvement of survival. quality, the effect of inhibiting cell proliferation

Inactive Publication Date: 2018-06-22
GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the curative effect of the CAC prevention and treatment method in the prior art is not very ideal and safe, the present invention provides an application of silibinin in the preparation of a drug for the prevention and treatment of colitis-related intestinal cancer

Method used

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  • Application of silibinin in preparation of drugs for preventing and treating intestinal cancer correlated with colitis
  • Application of silibinin in preparation of drugs for preventing and treating intestinal cancer correlated with colitis
  • Application of silibinin in preparation of drugs for preventing and treating intestinal cancer correlated with colitis

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Experimental program
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Effect test

Embodiment 1

[0025] The azoxymethane (AOM) / dextran sodium sulfate (DSS) mouse model is a mouse model of inflammatory bowel disease (inflammatory bowel disease) based on the synergistic effect of the carcinogen AOM and the inflammatory agent DSS. disease, IBD) has gradually developed into a research model of inflammation-related cancer of intestinal cancer.

[0026] Animal feeding methods are as follows:

[0027] AOM dissolved in physiological saline: In the first week of the experiment, according to the weight of each mouse, 10 mg / kg intraperitoneal injection;

[0028] Dissolve DSS in drinking water to a concentration of 2%: 10g DSS+500ml filtered autoclaved water;

[0029] SB dissolved in 0.5% hydroxymethylcellulose (CMC): 750mg / kg orally.

[0030] C57 6w Female mice (purchased from Beijing Animal Research Center)

[0031]

[0032] 2% DSS × 5 days, a total of 3 cycles

[0033] SB 5 days a week x 10 weeks

[0034] Record weight, diet and drinking water every week

[0035] All int...

Embodiment 2

[0038] MTT proliferation assay:

[0039] Cells with good growth status (HCT116 cell line, IMCE cell line) [HCT116 cell line was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai), and the mouse colonic pre-tumor cell line (Immorto-Min colonic epithelial cell line, IMCE) was purchased from Vanderbilt University, USA introduced], seeded in 96-well plate (5×10 3 each well) for 24 hours, given different concentrations of silibinin (50-800μ / M) to stimulate and continue to culture for 72 hours, and added 10 μL 5 mg / mL MTT [3-(4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide] 4 h, discard the culture medium, add 150 μl DMSO solution to each well, shake for 10 minutes to fully dissolve the crystals, and measure the OD value in a microplate reader at a wavelength of 570 nm. The experimental results showed that silibinin could well inhibit IMCE cells and HCT116 cells. (see image 3 , 4 )

Embodiment 3

[0041] Preparation of paraffin sections and evaluation of histological damage

[0042] The colon tissue specimens of each group were made into paraffin sections after being dehydrated, transparent, soaked in wax, embedded, sliced ​​and baked. After the sections are dewaxed and hydrated, hematoxylin staining, hydrochloric acid alcohol color separation, light ammonia water inversion blue and eosin staining are carried out in sequence, and then dehydration, transparency and sealing can be observed under the microscope and histological scoring (inflammation and tumor scores), the results showed that the silibinin intervention group significantly decreased the inflammation score and tumor score, and it was statistically significant (* p 0.001 , AOM / DSS vs AOM / DSS / SB). (see Figure 5 )

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Abstract

The invention discloses application of silibinin in preparation of drugs for preventing and treating intestinal cancer correlated with colitis. Silibinin can reduce the occurrence rate of AOM / DSS-induced mouse colonic inflammation and colonic tumors, decrease expression of mouse colonic inflammatory factors, reduce expression of inflammatory factors in a colonic cancer cell system, alleviate damage to a colonic mucosa barrier function, decrease proliferation of mouse colonic epithelial cells and suppress proliferation of the colonic cancer cell system. By lowering IL-6 / STAT3, the occurrence ofintestinal cancer correlated with colitis is suppressed. The application has a significant meaning on clinical prevention and treatment of intestinal cancer correlated with colitis, improvement of survival quality of patients and reduction of relapses of inflammation, and a new approach which is safe, effective and economical is provided for the prevention and treatment of intestinal cancer correlated with colitis.

Description

technical field [0001] The invention relates to the field of pharmaceutical preparations containing organic active ingredients, in particular to the application of silibinin in the preparation of drugs for preventing and treating intestinal cancer related to colitis. Background technique [0002] Silymarin is a class of flavonoids extracted from the fruit of milk thistle, mainly including Silybin (SB), Isosilybin, SilyChristin and Silydianin ), of which silybin is the main component of silymarin (accounting for 60%-70%) and an important active component. Milk thistle has been used to treat liver and gallbladder diseases for more than 2,000 years. As its main active component, silymarin can prevent The damage caused by chemical toxins, food toxins and drugs to the liver can promote the regeneration and repair of liver cells. It is called "natural liver protection medicine"; as a strong antioxidant, it can remove free radicals in the human body and delay aging. In recent year...

Claims

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Application Information

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IPC IPC(8): A61K31/357A61P35/00A61P1/00
CPCA61K31/357
Inventor 曹海龙王邦茂郑荣娟董文逍王斯南刘天宇陈雪马嘉珩金多晨
Owner GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
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