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Hrps Covalent tripolypeptide modified by Zn<++> and maltodextrin and preparation method thereof

A technology of maltodextrin and 3hrps-zn, which is applied in the field of Zn++ and maltodextrin double-modified Hrps covalent trimeric peptide and its preparation, can solve the biological activity constraints of simple active organic coordination compounds, difficult matching, easy Problems such as moisture absorption, photolysis and oxidation

Inactive Publication Date: 2018-07-10
四川国氏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The object of the invention is to provide a Zn ++ The Hrps covalent trimeric peptide double-modified with maltodextrin and its preparation method solve the problem that the existing Hrps polypeptide fragments are easy to absorb moisture, degrade, photolyze and oxidize under natural exposure conditions and because most of the active organic coordination compounds have a flexible structure , it is difficult to achieve an ideal match with the target, which makes the biological activity of purely active organic coordination compounds subject to certain constraints.

Method used

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  • Hrps Covalent tripolypeptide modified by Zn&lt;++&gt; and maltodextrin and preparation method thereof
  • Hrps Covalent tripolypeptide modified by Zn&lt;++&gt; and maltodextrin and preparation method thereof
  • Hrps Covalent tripolypeptide modified by Zn&lt;++&gt; and maltodextrin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Zn ++ A technical method for preparing double-modified covalent trimeric peptides by modifying several Hrps polypeptide combination fragments with maltodextrin (MD)

[0060] (1) The mass ratio (measured in mM) of fragments 1-4 of the Hrps functional polypeptide in the combination: usually the combination is composed of the same to different mM masses;

[0061] (2)Zn ++ Refers to the divalent salt of zinc. This test uses zinc sulfate with the molecular formula ZnSO 4 , molecular weight 161; selection of maltodextrin: degree of polymerization 6, 7 and 8, DE 5%-20%;

[0062] (3)Zn ++ Amphiphilic modification of Hrps protein functional polypeptide: a, 3 Hrps functional polypeptide fragments and Zn ++ The mass ratio: 6:1 to 1:1; b, reaction concentration: 3 Hrps polypeptide fragments and Zn ++ Mixing, 10-200mM phosphate buffer diluted to 10 to 100 times of the mixture quality; c, reaction pH and static treatment: 10-200mM phosphate buffer pH2-10, static treatm...

Embodiment 2

[0071] Example 2 Zn ++ and maltodextrin (MD) modified Hrps polypeptide fragments to prepare four combinations (3Hrps-Zn ++ ) n -(C 6 h 10 o 5 ) n Double modified covalent trimeric peptide dosage form design:

[0072] (3Hrps-Zn ++ ) n -(C 6 h 10 o 5 ) n (I-IV) The dosage forms designed for the preparation, storage and application of double-modified covalent trimeric peptides include: original drug, including solid or original powder and liquid or original solution; product dosage forms for storage and application include granules, wettable powders , water preparation, slow-release preparation and mixed preparations prepared with other fertilizers, or fungicides, insecticides or plant growth regulators, etc. In addition, (3Hrps-Zn ++ ) n -(C 6 h 10 o 5 ) n (I-IV) Relevant excipients and additives of double-modified covalent trimeric peptides, including food-grade soluble macronutrients and small amounts of nutrients. Since this kind of functional polypeptide c...

Embodiment 3

[0073] Example 3 Zn ++ and maltodextrin (MD) modified Hrps polypeptide fragments to prepare four combinations (3Hrps-Zn ++ ) n -(C 6 h 10 o 5 ) n The usage method and requirements of the double modified covalent trimeric peptide:

[0074] When using such functional polypeptide covalent polymers: (1) suitable methods for treating plants include seed soaking, seed dressing, seed coating, root dipping, foliage spraying, root application, injection, flower and fruit spraying, etc.; (2) treatment The suitable conditions for plants include choosing the appropriate time for medication and the environmental meteorological conditions during medication; granules, wettable powder or water should be formulated with chlorine-free river water, well water, groundwater or tap water to form the required concentration, and must not be mixed with acid, Alkali mixed, medicines should be kept in a cool and dry place; (3) The specific requirements for treating plants include that the medicine...

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Abstract

The invention discloses Hrps Covalent tripolypeptide modified by Zn<++> and maltodextrin and a preparation method thereof; three Hrps polypeptides are used as raw materials to form the covalent tripolypeptide via the dual modification of Zn<++> and maltodextrin; the covalent tripolypeptide has a structural general formula of (3Hrps-Zn<++>)n-(C6H10O5)n; the three Hrps polypeptides are any three ofamino acid sequences shown in SEQ ID NO: 1, SEQ ID NO:2, SEQ ID NO: 3 and SEQ ID NO: 4. The prepared Hrps Covalent tripolypeptide modified by Zn<++> and maltodextrin forms rigid structures mutually fitted in space and electric property and having better and more reasonable matching; the Hrps Covalent tripolypeptide has good emulsion stability, higher uniformity and higher structural stability, longer life, higher bioactivity, richer functionality and wider range of application.

Description

technical field [0001] The invention belongs to the field of biotechnology and mainly relates to Zn ++ A technical method for modifying three Hrps functional polypeptide combination fragments with maltodextrin, and through this technical method, Zn ++ , maltodextrin and several Hrps functional polypeptide fragments are covalently complexed to different degrees to generate complete and partially incomplete double-modified covalent trimeric peptides (3Hrps-Zn ++ ) n -(C 6 h 10 o 5 ) n , and the application of double-modified covalent trimeric peptide products in many fields. Background technique [0002] The cell surface of all organisms is covered by many different types of sugar chains, and various types of glycosylation also exist in the cells. The oligosaccharide chains are covalently linked to the amino acids in the protein polypeptide chains in various forms to form the glycopeptide linking chains of glycoproteins. Glycoproteins are fundamental substances involve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C07K1/107
CPCC07K14/21C07K14/27C07K2319/00
Inventor 吴伯骥吴彦
Owner 四川国氏生物科技有限公司