Method for extracting lycium barbarum polysaccharide

A wolfberry polysaccharide and extraction technology, applied in the field of polysaccharide extraction, can solve problems such as low purity, low extraction rate, and complicated operation, and achieve the effects of simple processing equipment, reduced acquisition time, and easy industrialization

Inactive Publication Date: 2018-07-13
邱媛媛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current extraction method of Lycium barbarum polysaccharide is complex in operation, low in extraction rate and low in purity, and needs further improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for extracting wolfberry polysaccharides, comprising the following steps:

[0016] A, take certain quality Chinese wolfberry, carry out vacuum freeze-drying and pulverize and cross 60 mesh sieves;

[0017] B. Take the dried wolfberry powder after sieving, add 700ml of water per 100g wolfberry according to the ratio of material to liquid and stir evenly;

[0018] C. Add cellulase and papain, and place in an ultrasonic extraction device to extract the enzymatic solution;

[0019] D. Filtrate the enzymolysis solution to obtain the filtrate, add the filtrate to absolute ethanol at a ratio of 1:4, set aside for 24 hours at 4°C, centrifuge for 15 minutes at a speed of 5000 rpm, collect the precipitate, freeze-dry it and pulverize it 80 mesh sieve to obtain soluble polysaccharide.

[0020] In this example, the addition amount of cellulase in step C is 0.2%, the addition amount of papain is 0.5%, the enzymolysis temperature is 55° C., and the pH value is 5.5.

[002...

Embodiment 2

[0023] A method for extracting wolfberry polysaccharides, comprising the following steps:

[0024] A, take the wolfberry of certain quality, carry out vacuum freeze-drying and pulverize and cross 80 mesh sieves;

[0025] B. Take the dried wolfberry powder after sieving, add 900ml water per 100g wolfberry according to the ratio of material to liquid and stir evenly;

[0026] C. Add cellulase and papain, and place in an ultrasonic extraction device to extract the enzymatic solution;

[0027] D. Filtrate the enzymolysis solution to obtain the filtrate, add the filtrate to absolute ethanol at a ratio of 1:4, set aside for 24 hours at 4°C, centrifuge for 15 minutes at a speed of 5000 rpm, collect the precipitate, freeze-dry it and pulverize it 80 mesh sieve to obtain soluble polysaccharide.

[0028] In this example, the addition amount of cellulase in step C is 0.4%, the addition amount of papain is 0.4%, the enzymolysis temperature is 60° C., and the pH value is 6.0.

[0029] I...

Embodiment 3

[0031] A method for extracting wolfberry polysaccharides, comprising the following steps:

[0032] A, take the wolfberry of certain quality, carry out vacuum freeze-drying and pulverize and cross 65 mesh sieves;

[0033] B. Take the dried wolfberry powder after sieving, add 750ml water per 100g wolfberry according to the ratio of material to liquid and stir evenly;

[0034] C. Add cellulase and papain, and place in an ultrasonic extraction device to extract the enzymatic solution;

[0035] D. Filtrate the enzymolysis solution to obtain the filtrate, add the filtrate to absolute ethanol at a ratio of 1:4, set aside for 24 hours at 4°C, centrifuge for 15 minutes at a speed of 5000 rpm, collect the precipitate, freeze-dry it and pulverize it 80 mesh sieve to obtain soluble polysaccharide.

[0036] In this example, the addition amount of cellulase in step C is 0.3%, the addition amount of papain is 0.5%, the enzymatic hydrolysis temperature is 56° C., and the pH value is 6.0.

...

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PUM

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Abstract

The invention discloses a method for extracting lycium barbarum polysaccharide. The method for extracting the lycium barbarum polysaccharide comprises the following steps: A, weighing a certain weightof lycium barbarum, performing vacuum freeze drying, crushing and passing through a sieve of 60 to 80 meshes; B, taking the sieved dried lycium barbarum powder, adding 700 to 900 ml of water into per100 g of lycium barbarum according to the material liquid ratio, and stirring uniformly; C, adding cellulase and papain, putting into ultrasonic extraction equipment and extracting enzymolysis liquid; D, filtering the enzymolysis liquid to obtain filtrate, adding absolute ethanol into the filtrate according to the ratio of 1:4, precipitating and standing at 4 DEG C for 24 hours, centrifuging for15 minutes at the centrifugal rotating speed of 5000 rpm, collecting the precipitate, performing freeze drying, crushing and passing through a 80-mesh sieve to obtain soluble polysaccharide. Enzymolysis is assisted by high-frequency ultrasonic treatment, so that the obtaining time of the lycium barbarum polysaccharide is greatly shortened, the polysaccharide extraction rate is relatively high on the premise of guaranteeing the purity of the lycium barbarum polysaccharide, treatment equipment is simple, a toxic organic solvent is not used, and industrialization is facilitated.

Description

technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to a method for extracting wolfberry polysaccharide. Background technique [0002] Lycium barbarum polysaccharide is a water-soluble polysaccharide. It has been clarified that the polysaccharide is a proteoglycan, with a molecular weight of 22-25kD, composed of six monosaccharide components: arabinose, glucose, acetic polysaccharide, galactose, mannose, xylose, and rhamnose, with a content of 20%, 50% (UV ). Pale yellow powder, easy to absorb moisture. Water solubility: soluble in water, no precipitation Heavy metal indicators: As<1.0ppm, Cd<2ppm, Cr<1ppm, Pb<2ppm, Hg<0.5ppm Hygienic indicators: bacteria count<1000 / g Escherichia coli and live mites are not allowed to be tested The number of molds produced <100 / g Loss on drying: ≤9%; It has physiological activity, can enhance non-specific immune function, improve disease resistance, inhibit t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/0003
Inventor 邱媛媛
Owner 邱媛媛
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