Fluorescent oxygen probe, and preparation method and use thereof

Abstract

The invention provides a fluorescent oxygen probe which includes polymer microspheres copolymerized with a reference probe compound, and a fluorescent oxygen probe compound uniformly dispersed in thepolymer microspheres copolymerized with the reference probe compound. The invention also provides a method for creatively preparing the fluorescent oxygen probe. By copolymerizing the reference probecompound and a polymerized monomer, the microspheres dispersed with the fluorescent oxygen probe compound are prepared and can be used as a fluorescent oxygen probe for use. The prepared fluorescent oxygen probe has a self reference effect, and the problem that a fluorescent probe needs to be recalibrated when a detection device is replaced in traditional fluorescence detection is avoided. In addition, the fluorescent oxygen probe has no high requirements on a detection environment and can be used for detecting the concentration of dissolved oxygen in a dynamic biological environment, such asa water body containing microorganisms, and the error of the detection result is less than or equal to 5% compared with that of a traditional electrochemical detection equipment.

Description

technical field [0001] The invention relates to the field of fluorescence detection, in particular to a fluorescent oxygen probe and its preparation method and application. Background technique [0002] Oxygen is an indispensable substance in life and is closely related to human life. How to quickly and accurately detect oxygen concentration is of great significance in fisheries, food safety, environmental testing, and biomedicine. [0003] So far, there are many methods for detecting oxygen concentration. Specific detection methods such as chemical titration detection, electrochemical detection, fluorescence detection, etc., the traditional chemical titration method has the highest detection accuracy, but the traditional chemical titration method needs to be equipped with a standard concentration solution, the steps are cumbersome, time-consuming, and complicated to operate. It is only suitable for some precise tests. The electrochemical detection method obtains the oxygen ...

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Problems solved by technology

[0003] So far, there are many methods for detecting oxygen concentration. Specific detection methods such as chemical titration detection, electrochemical detection, fluorescence detection, etc., the traditional chemical titration method has the highest detection accuracy, but the traditional chemical titration method needs to be equipped with a standard concentration solution, the steps are cumbersome, time-consuming, and complicated to operate. It is only suitable for some precise tests. The electrochemical detection method obtains the oxygen concentration by detecting the reduction potential of oxygen at the anode. It has a good application in the detection of dissolved oxygen, but its The current is easily affected by many factors such as electromagnetic interference, and the detection results have certain accidental errors. Moreover, limited by the size of the equipment, this method cannot be applied to some small areas. Fluorescence detection is a new detection method. Due to its It has many advantages such as high efficiency, good selectivity, convenient detection, and short response time, and is favored by the fields of analytical chemistry, bioanalytical chemistry, and environmental science.

[0004] Fluorescence detection methods usually require the participation of fluorescent probes. When the fluorescent probe is combined with the sample to be tested, its fluorescence intensity or fluorescence emission peak needs to change significantly, and the change can be detected by the fluorescence detector. However, the existing technology The use of fluorescence detection method to detect oxygen concentration usually faces a problem, that is, most of the fluorescent substances that can be used as fluorescent probes to detect oxygen are relatively poor in stability and prone to photobleaching, resulting in a decrease in fluorescence efficiency and large deviations in the detection results. , and because the fluorescence intensity units of various types of fluorescence spectrometers are not uniform, the fluorescent probes can usually only be used with specific types of fluorescence spectrometers. The standard curve can only be used after re-making the standard curve. The above shortcomings limit the further promotion of the fluorescence detection method.

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