Fluorescent oxygen probe, and preparation method and use thereof

An oxygen probe and fluorescence technology, applied in the field of fluorescence detection, can solve the problems of deviation of detection results, decrease of fluorescence efficiency, complicated operation, etc., and achieve the effect of reducing detection error and preventing metabolic decomposition

Active Publication Date: 2018-07-27
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] So far, there are many methods for detecting oxygen concentration. Specific detection methods such as chemical titration detection, electrochemical detection, fluorescence detection, etc., the traditional chemical titration method has the highest detection accuracy, but the traditional chemical titration method needs to be equipped with a standard concentration solution, the steps are cumbersome, time-consuming, and complicated to operate. It is only suitable for some precise tests. The electrochemical detection method obtains the oxygen concentration by detecting the reduction potential of oxygen at the anode. It has a good application in the detection of dissolved oxygen, but its The current is easily affected by many factors such as electromagnetic interference, and the detection results have certain accidental errors. Moreover, limited by the size of the equipment, this method cannot be applied to some small areas. Fluorescence detection is a new detection method. Due to its It has many advantages such as high efficiency, good selectivity, convenient detection, and short response time, and is favored by the fields of a

Method used

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  • Fluorescent oxygen probe, and preparation method and use thereof
  • Fluorescent oxygen probe, and preparation method and use thereof
  • Fluorescent oxygen probe, and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Fluorescent oxygen probe 1 was prepared by the following steps:

[0049] Synthesis of the reference probe compound rhodamine B acrylate: 400 mg of rhodamine B and 261 mg of triethylamine were dissolved in 20 ml of tetrahydrofuran to obtain rhodamine B solution, and then 2 mL of tetrahydrofuran with 135 mg of methacrylamide dissolved in 0 Slowly drop into the rhodamine B solution in an ice bath at ~5°C, stir overnight at room temperature to react, pour the product into excess water after the reaction, extract with dichloromethane to obtain an organic phase, wash the organic phase with pure water three times, Anhydrous MgSO 4 Dried to obtain the crude product, the crude product was separated by silica gel column chromatography, with dichloromethane and methanol in a volume ratio of 95:5 as eluent chromatography to obtain 300mg dark red viscous substance, which was the rhodamine acrylate B ester, the reaction yield is 70.10%.

[0050] The hydrogen nuclear magnetic resona...

Embodiment 2

[0054] Fluorescent oxygen probe 2 was prepared by the following steps:

[0055] The only difference with Example 1 is that the reference probe compound in step (1) is selected from (7-hydroxycoumarin) acrylate, the addition of the reference probe compound is 250mg, and the solvent of the reference probe compound is selected N,N-Dimethylformamide.

[0056] Example 2 Obtain the fluorescent oxygen probe 2.

Embodiment 3

[0058] Fluorescent oxygen probe 3 was prepared by the following steps:

[0059] The only difference from Example 1 is that tris(1,10-phenanthroline)ruthenium(II) is selected as the fluorescent oxygen probe compound in step (1), and the added amount of the fluorescent oxygen probe compound is 1 g.

[0060] Example 3 obtained the fluorescent oxygen probe 3.

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Abstract

The invention provides a fluorescent oxygen probe which includes polymer microspheres copolymerized with a reference probe compound, and a fluorescent oxygen probe compound uniformly dispersed in thepolymer microspheres copolymerized with the reference probe compound. The invention also provides a method for creatively preparing the fluorescent oxygen probe. By copolymerizing the reference probecompound and a polymerized monomer, the microspheres dispersed with the fluorescent oxygen probe compound are prepared and can be used as a fluorescent oxygen probe for use. The prepared fluorescent oxygen probe has a self reference effect, and the problem that a fluorescent probe needs to be recalibrated when a detection device is replaced in traditional fluorescence detection is avoided. In addition, the fluorescent oxygen probe has no high requirements on a detection environment and can be used for detecting the concentration of dissolved oxygen in a dynamic biological environment, such asa water body containing microorganisms, and the error of the detection result is less than or equal to 5% compared with that of a traditional electrochemical detection equipment.

Description

technical field [0001] The invention relates to the field of fluorescence detection, in particular to a fluorescent oxygen probe and its preparation method and application. Background technique [0002] Oxygen is an indispensable substance in life and is closely related to human life. How to quickly and accurately detect oxygen concentration is of great significance in fisheries, food safety, environmental testing, and biomedicine. [0003] So far, there are many methods for detecting oxygen concentration. Specific detection methods such as chemical titration detection, electrochemical detection, fluorescence detection, etc., the traditional chemical titration method has the highest detection accuracy, but the traditional chemical titration method needs to be equipped with a standard concentration solution, the steps are cumbersome, time-consuming, and complicated to operate. It is only suitable for some precise tests. The electrochemical detection method obtains the oxygen ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 田颜清梁兰锋李刚毛永云
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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