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Mixotrophic culture method for producing polyunsaturated fatty acid and fucoxanthin by utilizing phaeodactylum tricornutum

A kind of technology of unsaturated fatty acid and fucoxanthin, applied in the field of bioengineering

Active Publication Date: 2018-07-31
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on whether mannitol is suitable as a carbon source for adjunct microorganisms and how to use mannitol to greatly increase the growth rate of adjunct microorganisms

Method used

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  • Mixotrophic culture method for producing polyunsaturated fatty acid and fucoxanthin by utilizing phaeodactylum tricornutum
  • Mixotrophic culture method for producing polyunsaturated fatty acid and fucoxanthin by utilizing phaeodactylum tricornutum
  • Mixotrophic culture method for producing polyunsaturated fatty acid and fucoxanthin by utilizing phaeodactylum tricornutum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1 Mannitol concentration experiment

[0073] The method for concurrently cultivating Phaeodactylum tricornutum to produce polyunsaturated fatty acids and fucoxanthin, the steps are as follows:

[0074] (1) Cultivate Phaeodactylum tricornutum to logarithmic growth phase (temperature 24°C, 80 μmol photons m −2 ·s −1 cultivated under light conditions for 3 days) to obtain seed solution;

[0075] (2) Inoculate the seed solution prepared in step (1) into the culture medium at a volume ratio of 5-50%, and inoculate the medium at a temperature of 23-25°C and 100 μmol photons·m −2 ·s −1 Cultivate under light conditions for 10 days, and the photoperiod is 16h light and 8h dark;

[0076] For the culture medium in the step (2), prepare the culture medium whose carbon source is 0.5, 1, 2, 4, 10 and 15 g / L of mannitol, and take no mannitol as the control, the above medium The other ingredients are as follows per liter:

[0077] 1 g NaNO 3 , 30 g sea salt, 4.37 mg N...

Embodiment 2

[0079] Embodiment 2 tea polyphenols concentration experiment

[0080] The method for concurrently cultivating Phaeodactylum tricornutum to produce polyunsaturated fatty acids and fucoxanthin, the steps are as follows:

[0081] (1) Cultivate Phaeodactylum tricornutum to logarithmic growth phase (temperature 24°C, 80 μmol photons m −2 ·s −1 cultivated under light conditions for 3 days) to obtain seed solution;

[0082] (2) Inoculate the seed solution prepared in step (1) into the culture medium at a volume ratio of 5-50%, and inoculate the medium at a temperature of 23-25°C and 100 μmol·photons·m −2 ·s −1 Cultured under light conditions for 8 days, with a photoperiod of 16 h light and 8 h dark;

[0083] For the culture medium in the step (2), prepare medium containing tea polyphenols at a concentration of 0.05, 0.1, 0.2, 0.5, 1.0, 2.0 and 5.0 g / L respectively, and without adding tea polyphenols as a control, the above culture The content per liter based on other ingredients...

Embodiment 3

[0087] As in the method described in Example 1, the difference is that the contents of each liter of medium components are as follows:

[0088]2 g mannitol, 0.2 g / L tea polyphenols, 10 g NaNO 3 , 10g sea salt, 10 mg Na 2 EDTA, 1 mg FeCl 3 •6H 2 O, 12 mg NaH 2 PO 4 •12H 2 O, 10 mg Na 2 SiO 3 •7H 2 O, 0.1 mg CuSO 4 • 5H 2 O, 0.002 mg MgSO 4 •7H 2 O, 0.1mg CoCl 2 •6H 2 O, 0.02 mg MnCl 2 • 4H 2 O, 0.01 mg Na 2 MoO 4 • 2H 2 O, 0.0001 mg biotin.

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Abstract

The invention relates to a mixotrophic culture method for producing polyunsaturated fatty acid and fucoxanthin by utilizing phaeodactylum tricornutum. The invention relates to application of mannitolin producing the polyunsaturated fatty acid and the fucoxanthin by mixotrophic culture of diatom and application of tea polyphenol or active ingredients of the tea polyphenol in producing the polyunsaturated fatty acid and the fucoidan by the mixotrophic culture of the diatom. According to the mixotrophic culture method disclosed by the invention, the mannitol is adopted as a mixotrophic carbon source for the first time; through experiments, the mannitol is found to have the advantage that the growth rate and the yield of the phaeodactylum tricornutum can be effectively improved when being compared with other carbon sources; in addition, mixotrophic culture has better synthesis efficiency than that of heterotrophic culture in pigment synthesis level of a photosynthetic system. Besides, according to the mixotrophic culture method for producing the polyunsaturated fatty acid and the fucoxanthin by utilizing the phaeodactylum tricornutum, disclosed by the invention, the tea polyphenol isused as a bioactive substance to culture the diatoms, and can effectively promote absorption and utilization of the carbon sources; through actual detection, the biomass of the phaeodactylum tricornutum can be increased by 108 percent, the content of the fucoxanthin is increased by 96 percent and the content of EPA is increased by 122 percent.

Description

technical field [0001] The invention relates to a concurrent culture method for producing polyunsaturated fatty acid and fucoxanthin by using Phaeodactylum tricornutum, belonging to the technical field of bioengineering. Background technique [0002] Fucoxanthin, also known as fucoxanthin or fucoxanthin, is an oxygen-containing derivative of carotenoids and is commonly found in brown algae and diatoms. Fucoxanthin mainly performs the function of capturing light energy in the photosynthetic system in algae cells. Fucoxanthin and Chlorophyll a and chlorophyll c Composition of fucoxanthin - chlorophyll protein complex (Fucoxanthin-ChlorophyllProtein, FCP). In recent studies, fucoxanthin has been proven to be a safe and effective dietary supplement, which can promote metabolism without affecting the nervous system, and has anti-obesity, anti-diabetes, anti-oxidation, anti-inflammatory, anti-tumor, etc. A variety of physiological activities [1]. A large number of experimenta...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12P17/02C12N1/38C12N1/12C12R1/89
CPCC12N1/12C12N1/38C12P7/6427C12P17/02
Inventor 范勇李福利胡光荣王丽娟
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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