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Decellularized tilapia skin biomimetic substrate for soft tissue restoration as well as preparation method and application thereof

A tilapia and decellularization technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as difficult quality control or risk monitoring, safety in clinical use, sensitization or immune rejection, etc. cost, good industrialization market potential, and the effect of reducing the risk of immunogenicity

Pending Publication Date: 2018-08-03
SHANGHAI SIXTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the tilapia skin used in this report only underwent simple descaling, deredundancy, deodorization and sterilization, but did not remove highly immunogenic cells, and there is still the possibility of sensitization or immune rejection. There are potential safety hazards in clinical use; the tilapia skin used still retains a large amount of pigment and other components, which makes each piece of fish skin very different, making it difficult to carry out quality control or risk monitoring, and cannot be used as a large-scale production of medical products. marketing

Method used

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  • Decellularized tilapia skin biomimetic substrate for soft tissue restoration as well as preparation method and application thereof
  • Decellularized tilapia skin biomimetic substrate for soft tissue restoration as well as preparation method and application thereof
  • Decellularized tilapia skin biomimetic substrate for soft tissue restoration as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of acellular tilapia skin biomimetic matrix for soft tissue repair

[0042] 1) Tilapia skin pretreatment: fresh tilapia skin is scraped off residual scales and redundant muscle tissues, rinsed with deionized water at 4°C several times to remove residual blood; cut into appropriate sizes according to different anatomical parts, and placed quickly Freeze at -40°C, take out and thaw under running water at room temperature before use;

[0043] 2) Pretreatment: at 4°C, stir with a 3.5% NaCl solution with a solid-liquid ratio of 1:20 (w / w) for 1 hour and then remove the waste liquid, repeating twice; Citric acid solution (0.005mol / L) was treated for 2 hours; the tilapia skin was roughly sorted to remove some redundancy;

[0044] 3) Microstructure protection: at 4°C, stir with PBS buffer solution (0.1M, pH7.4) with a solid-to-liquid ratio of 1:20 (w / w) for 2 hours and then remove the waste liquid; repeat the above steps twice;

[0045] 4) Cleaning: at 4...

Embodiment 2

[0052] Example 2 Preparation of acellular tilapia skin biomimetic matrix for soft tissue repair

[0053] 1) tilapia skin pretreatment: with embodiment 1;

[0054] 2) Pretreatment: at 4°C, stir 5% NaCl solution with a solid-liquid ratio of 1:10 (w / w) for 2 hours, then remove the waste liquid, and repeat twice; Citric acid solution (0.01mol / L) was treated for 2 hours; the tilapia skin was roughly sorted to remove some redundancy;

[0055] 3) Microstructure protection: at 4°C, stir with PBS buffer solution (0.2M, pH7.0) with a solid-to-liquid ratio of 1:10 (w / w) for 2 hours and then remove the waste liquid; repeat the above steps twice;

[0056] 4) Cleaning: at 4°C, stir with a 5% NaCl solution with a solid-to-liquid ratio of 1:10 (w / w) for 4 hours and then remove the waste liquid;

[0057] 5) Initial decellularization treatment: spread the above-mentioned tilapia skin on a dry-baked and sterilized tray, quickly freeze it at -40°C for 4 hours, and thaw it under clean conditions...

Embodiment 3

[0063] Example 3 Preparation of acellular tilapia skin biomimetic matrix for soft tissue repair

[0064] 1) tilapia skin pretreatment: with embodiment 1;

[0065] 2) pretreatment: with embodiment 2;

[0066] 3) Microstructure protection: at 4°C, stir for 2 hours with D-Hank’s buffer solution (pH7.2) with a solid-to-liquid ratio of 1:10 (w / w) and then remove the waste liquid; repeat the above steps twice;

[0067] 4) Cleaning: at 4°C, stir with a 4% NaCl solution with a material-to-liquid ratio of 1:10 (w / w) for 4 hours and then remove the waste liquid;

[0068] 5) Initial decellularization treatment: same as in Example 1;

[0069] 6) Microstructure re-protection: at 4°C, stir with D-Hank’s buffer solution (pH7.2) with a solid-to-liquid ratio of 1:10 (w / w) for 2 hours and then remove the waste liquid; repeat the above steps twice;

[0070] 7) Secondary decellularization treatment: at 4°C, stir for 18 hours with 1% sodium deoxycholate (containing gentamicin sulfate 50 μg / ml) ...

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Abstract

The invention discloses a decellularized tilapia skin biomimetic substrate for soft tissue restoration as well as a preparation method and application thereof, and belongs to the field of a medical material. The decellularized tilapia skin biomimetic substrate is obtained by using the tilapia skin as raw materials through special treatment. A preparation method of the decellularized tilapia skin biomimetic substrate for soft tissue restoration comprises the process steps of raw material pretreatment, coarse finishing, decellularization, refinishing, storage liquid pretreatment, shaping and thelike. The decellularized tilapia skin biomimetic substrate for soft tissue restoration prepared by the method has the advantages that the network structure is complete; the flexibility is good; the substrate belongs to a low-immunogenicity biomimetic extracellular substrate stent material, has good biological function and physical performance, and can be used for restoration of various soft tissues such as burn and scald wound surfaces, ulceration wound surface, hernia repair, duramater reparation, urethra / bladder / pelvic floor reconstruction, periodontium restoration and mucosal tissue restoration.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a biomimetic matrix of acellular tilapia skin for soft tissue repair and a preparation method and application thereof. Background technique [0002] Repair of soft tissue injury is a common trauma surgery disease, the etiology and injury factors are diverse, and it is a thorny problem that many clinical departments need to solve urgently. Traditional treatment methods have poor prognosis and are prone to complications such as wound infection, and even disability or death in severe cases. In addition, some soft tissue injuries that need to be repaired by tissue engineering are limited by the lack of materials or high cost, so it is difficult to be widely promoted as a common clinical path. The repair of soft tissue injury has become one of the research hotspots. [0003] Common soft tissue repair materials include polymer materials, biomaterials, and synthetic mat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/50
CPCA61L27/3633A61L27/3641A61L27/3687A61L27/3691A61L27/50A61L2430/34A61L2430/40
Inventor 位晓娟张长青张伟
Owner SHANGHAI SIXTH PEOPLES HOSPITAL
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