Sipunculid peptide and application thereof to preparation of gestational period hypertension treatment medicine

A technology of gestational hypertension and Astrocyst, which is applied in the field of astronomy peptide and its application in the preparation of drugs for the treatment of gestational hypertension, which can solve the problems of no cure, lack of methods, and caution in treatment, and achieve a clear mechanism of action , good control effect, high safety effect

Active Publication Date: 2018-08-03
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is to provide the use and application method of astronomy peptides in the preparation of products for the treatment of hypertension during pregnancy in view of the lack of methods for the treatment of hypertension during pregnancy, the lack of a permanent cure, and the need for caution in treatment.

Method used

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  • Sipunculid peptide and application thereof to preparation of gestational period hypertension treatment medicine
  • Sipunculid peptide and application thereof to preparation of gestational period hypertension treatment medicine
  • Sipunculid peptide and application thereof to preparation of gestational period hypertension treatment medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0013] Dissect the star worm, remove the internal organs, clean the star worm body, add an appropriate amount of deionized water according to the ratio of worm body: deionized water = 1:20 (m / V), pour it into a tissue masher, Stir at high speed to mash, then sonicate in an ice bath. After the ultrasonication is completed, put it into a 50°C water bath and stir to extract water-soluble proteins, then coarsely filter with gauze to remove the residue, centrifuge the solution (6000rpm, 15min), collect the supernatant, and put the supernatant in a freeze dryer. Dry to a protein powder.

[0014] The protemax proteolytic enzyme produced by Novozymes was used, and the ratio of enzyme to substrate concentration was (0.4AU:1g) for hydrolysis. First, 1000ml of star worm protein extraction solution was heated to a constant temperature in a constant temperature water bath, the temperature was 50 ° C, and the pH was adjusted. is about 7.0, then add proteolytic enzyme and stir for 4h. Afte...

Embodiment 2

[0017] Pregnant SD rats were randomly divided into three groups: normal control group, L-nitrite L-arginine methyl ester (L-NAME) model group, and astrocystic peptide gavage group. On the sixth day of co-cage, the animals in the experimental group were gavaged with 50 mg / d.kg.bw celestial peptide, and the normal control group and L-NAME group were gavaged with the same amount of normal saline every day until the end; -NAME group and experimental group were injected subcutaneously with L-Nitrite-L-arginine methyl ester 125 mg / d·kg·bw every day, and the normal control group was injected with the same amount of normal saline. On the 21st day, blood pressure and urine protein were measured. Then the femoral artery was sacrificed by bloodletting, the abdominal aorta blood of each group was collected, the serum was separated, and the superoxide (MDA), nitric oxide (NO), superoxide dismutase (SOD), nitric oxide (NO) in serum were detected. Enzyme (NOS) enzymatic activity. At the sa...

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Abstract

The invention discloses application of sipunculid peptide to preparation of gestational period hypertension treatment or prevention products. The amino acid sequence of the sipunculid peptide is His-Thr-Arg-Asp-Gly. The sipunculid peptide can effectively reduce the blood pressure and the urine protein of a mouse with gestational period hypertension caused by L-nitrous acid L-arginine methyl ester,inhibit the increase of the oxidative stress index of the mouse with gestational period hypertension and can also reduce the up-regulation of inflammatory marker molecules. The sipunculid peptide isa compound capable of effectively intervening the gestational period hypertension diseases generated during the woman gestational period. The sipunculid peptide has high use safety on users-pregnant women with gestational period hypertension diseases, and has the characteristics of no toxicity and low side effect. The sipunculid peptide can be widely used in pharmacy, heath care products and food,and is used for prevention and treatment.

Description

technical field [0001] The invention belongs to the technical field of functional polypeptide screening, and in particular relates to an astrocytic peptide and its application in preparing a drug for treating gestational hypertension. Background technique [0002] Gestational hypertension is a serious obstetric disease unique to pregnancy, mainly manifested as hypertension and proteinuria, and is one of the main causes of increased maternal and perinatal mortality. In recent years, studies on its pathogenesis suggest that abnormal oxidative stress in pregnant women is a risk factor for gestational hypertension. The toxic effects of oxidative stress cause serious damage to the vascular endothelial cells, cell membranes and vascular linings of pregnant women, resulting in abnormally elevated blood pressure. In addition, excessive inflammation in the blood vessels of gestational hypertension also leads to endothelial cell damage. However, the current clinical treatment methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P9/12A61P15/00A23L33/18
CPCA23V2002/00A61K38/00A23L33/18C07K7/06A23V2200/30A23V2200/326A23V2250/55
Inventor 陈海敏杨锐陈娟娟骆其君贾福怀熊菲菲
Owner NINGBO UNIV
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