Method for remediating polychlorinated biphenyl-polluted soil by utilizing arbuscular mycorrhizal fungi and combining pig-derived biochar

A technology of arbuscular mycorrhizal fungi and polychlorinated biphenyls is applied in the field of remediation of polluted soil, which can solve the problems of reducing bioavailability, lack of, and changing the activity of soil microbial community, so as to promote the degradation rate, increase the effective phosphorus content, and promote the Effect of Mycorrhizal Fungal Infection Rate

Active Publication Date: 2018-08-07
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biochar has become a hot topic in the field of environmental remediation in recent years. Due to its strong adsorption capacity, it can fix organic pollutants in the soil and reduce its bioavailability.
At the same time, biochar can also change the soil microbial community and its activity, thereby affecting the degradation of soil organic pollutants.
[0004] However, the combined remediation of arbuscular mycorrhizal fungi and biochar on PCB-contaminated soil lacks corresponding methods. Traditional fungi have an inhibitory effect on plant root biomass, and cannot effectively improve the abundance of soil microorganisms, and cannot effectively To promote the degradation of PCBs in soil, therefore, a method of using arbuscular mycorrhizal fungi and pig charcoal to jointly remediate PCB-contaminated soil was proposed to solve the above problems

Method used

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  • Method for remediating polychlorinated biphenyl-polluted soil by utilizing arbuscular mycorrhizal fungi and combining pig-derived biochar
  • Method for remediating polychlorinated biphenyl-polluted soil by utilizing arbuscular mycorrhizal fungi and combining pig-derived biochar
  • Method for remediating polychlorinated biphenyl-polluted soil by utilizing arbuscular mycorrhizal fungi and combining pig-derived biochar

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0025] Preparation of pig charcoal in step B: use a pyrolysis device to pyrolyze a dead whole pig at 650°C under anoxic conditions, grind it into fine particles, and sieve it with a stainless steel sieve with a particle size of 0.25 mm.

[0026] The particle size of the sown pig charcoal is one of less than 0.25 mm or greater than 0.25 mm.

[0027] The bacterial strain is Physiocystis mosesi with the preservation number M47V.

[0028] The soil for the test was collected from an electronic waste dismantling site in Taizhou City, Zhejiang Province. The surface soil 0-20cm was collected, gravel and other debris were removed, and it was fully mixed after natural air drying. The basic physical and chemical properties of the mixed soil are pH 8.17 and organic carbon 15.34g kg -1 , available phosphorus 16.84mg kg -1 , available potassium 112.19mg kg -1 , Soil PCB concentration 181.14mgkg -1 , is seriously polluted; the tested corn is Jingke Nuo 2000, which was purchased from a lo...

Embodiment 1

[0030] With no inoculation of arbuscular mycorrhizal fungus and no addition of pig charcoal as a control, take 1300 g of air-dried PCB-contaminated soil and put it into a plastic pot with a capacity of 2.5 L, mix it evenly and add water until the soil moisture content reaches the maximum sustainable value in the field. 60% of the amount of water; select corn seedlings with similar growth, and transplant each three as a group into each potted plant to cultivate the corn seedlings. The potted plants are calculated from transplanting and grow for 90 days at normal temperature; after the cultivation period ends, Plants were harvested and soil samples were taken for analysis of corn biomass, soil PCB content, and microbes.

Embodiment 2

[0032] Take 1300g of air-dried PCB-contaminated soil and 37.1g of pig charcoal with a particle size less than 0.25mm into a plastic pot with a capacity of 2.5L, mix evenly and add water until the soil moisture is 60% of the maximum water holding capacity in the field; choose Corn seedlings with similar growth are transplanted into each potted plant as a group of three, and the corn seedlings are cultivated. The potted plants are counted from the time of transplanting and grown at room temperature for 90 days; after the cultivation period, the plants are harvested and soil samples are taken , analysis of corn biomass, soil PCB content, and microorganisms.

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Abstract

The invention discloses a method for remediating polychlorinated biphenyl-polluted soil by utilizing arbuscular mycorrhizal fungi and combining pig-derived biochar. The method comprises the followingsteps: selecting 0-20cm surface soil of the polychlorinated biphenyl-polluted soil, removing sundries in the soil, and carrying out air drying under natural conditions for later use; sowing the soil,the arbuscular mycorrhizal fungi and the pig-derived biochar in a mass ratio of 35:4:1 under ambient temperature condition, turning over, uniformly mixing, and watering to 60% of maximum water-holdingcapacity of the soil, so that degradation of polychlorinated biphenyl in the soil is realized. The arbuscular mycorrhizal fungi and the pig-derived biochar respectively obviously increase effective phosphorus content of the soil, and the pig-derived biochar also obviously increases organic carbon in the soil, rapidly available potassium content and pH value; the pig-derived biochar obviously promotes infection rate of mycorrhiza fungi, and the arbuscular mycorrhizal fungi are inoculated and the pig-derived biochar is added, so that abundance of bacteria is improved; the pig-derived biochar with the particle size larger than 0.25mm is added while the arbuscular mycorrhizal fungi are inoculated, degradation rate of polychlorinated biphenyl in the soil is obviously improved, and the degradation of plant biomass and polychlorinated biphenyl in the soil is promoted.

Description

technical field [0001] The invention relates to a method for remediating polluted soil, in particular to a method for jointly remediating PCB-contaminated soil by using arbuscular mycorrhizal fungi and pig charcoal, and belongs to the technical field of environmental protection applications. Background technique [0002] Polychlorinated biphenyls are a typical persistent organic pollutant with high chemical and thermal stability, oxidation resistance, high hydrophobicity and potential biological carcinogenic effects, which cause them to exist in the soil for a long time and pass through the biological chain. The enrichment effect of concentrated and amplified substances enters the food chain, which has a major impact on human health. Therefore, remediation of PCB-contaminated soil has become a key issue that needs to be solved urgently. [0003] Arbuscular mycorrhizal fungi are a class of plant symbiotic fungi that widely exist in nature and have been widely used in the bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/00B09C1/10
CPCB09C1/00B09C1/105B09C2101/00
Inventor 秦华单明娟邬奇峰
Owner ZHEJIANG FORESTRY UNIVERSITY
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