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Carotane sesquiterpene compound as well as preparation and application thereof

A technology of sesquiterpenes and carotene, which is applied in the field of carotane sesquiterpenes and its preparation, can solve the problems of non-red tide biological hazards, environmental secondary pollution, drug residues, etc.

Active Publication Date: 2018-08-10
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although chemicals such as copper sulfate and chlorine can quickly and effectively kill or inhibit harmful algae or inhibit their growth and reproduction, there are problems such as harm to non-red tide organisms, drug residues, and secondary pollution to the environment.

Method used

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  • Carotane sesquiterpene compound as well as preparation and application thereof
  • Carotane sesquiterpene compound as well as preparation and application thereof
  • Carotane sesquiterpene compound as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The structure of carotane sesquiterpenoids derived from algal epiphyte fungi is shown in formula (I).

[0026]

[0027] This compound has the following physicochemical and spectral properties:

[0028] Colorless oil; specific rotation [α] 20 D –114 (c 0.13, MeOH); H NMR spectrum (solvent is deuterated chloroform) δ H 2.29(d, 16.1), 2.25(d, 16.1), 2.41(dd, 12.0, 3.7), 2.61(m), 2.51(dddd, 20.1, 7.1, 3.7, 0.9), 6.43(dq, 7.1, 1.4), 2.84(d,15.7), 2.64(d,15.9), 1.78(heptet,6.9), 0.97(d,6.9), 0.91(d,6.8), 1.89(br s), 1.02(s); CNMR (solvent is deuterated chloroform) δ C 36.4(C), 54.1(CH 2 ), 218.5(C), 81.2(C), 48.6(CH), 28.4(CH 2 ), 139.3(CH), 137.3(C), 201.3(C), 57.8(CH 2 ),36.7(CH),16.8(CH 3 ), 17.2(CH 3 ), 22.2 (CH 3 ), 20.2 (CH 3 ); high resolution mass spectrometry [M] + m / z 250.1570, calculated 250.1569.

Embodiment 2

[0030] The preparation method of carotane sesquiterpenoids as shown in formula (I):

[0031] Take the well-grown Trichoderma virens Y13-3 strain on the plate, cut it into small pieces and inoculate it in the potato dextrose liquid medium, put 300 ml of culture medium in each 1 liter conical flask, a total of 200 bottles, room temperature After static fermentation for 30 days, it was extracted three times with ethyl acetate and concentrated under reduced pressure to obtain 27.2 g of crude extract after concentration.

[0032] The potato glucose liquid medium is composed of 500 ml of boiled juice containing 100 grams of potatoes per liter, 20 grams of glucose, 5 grams of peptone, 5 grams of yeast extract and 500 ml of Chenhai seawater.

[0033] Trichoderma virens Y13-3 strain was preserved in CCTCC, China Type Culture Collection Center on January 10, 2018, address: Wuhan University, China, preservation number is CCTCC NO: M 2018016, classified as Trichoderma virens, The strain ...

Embodiment 3

[0038] The difference from Example 2 is that

[0039] Take the well-grown Trichoderma virens Y13-3 strain on the plate, cut it into small pieces and inoculate it in Jerusalem artichoke glucose liquid medium, put 300 ml of culture medium in each 1 liter conical flask, a total of 100 bottles, room temperature The fermentation was static for 40 days, and the mycelium and fermentation broth were collected by filtration and separately.

[0040] The Jerusalem artichoke glucose liquid medium is composed of 500 ml of boiled juice containing 100 grams of Jerusalem artichoke tubers per liter, 20 grams of glucose, 5 grams of peptone, 5 grams of yeast extract, and 500 milliliters of old seawater.

[0041] About 30 liters of fermentation broth were collected, extracted three times with ethyl acetate, and concentrated under reduced pressure; the mycelium was dried and pulverized, and then extracted three times with ethyl acetate, and concentrated under reduced pressure; the concentrate was de...

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Abstract

The invention relates to the field of algae inhibitors, in particular to a carotane sesquiterpene compound of an alga growth fungus source as well as a preparation and application thereof to inhibition of microalgae. A specific structural formula is shown in (I) shown in the description. The preparation method comprises the following steps: inoculating alga growth fungus trichoderma virens Y13-3 into a fungus medium for performing fermentation culture, separating and purifying the fermentation product, thereby obtaining the carotane sesquiterpene compound shown as the formula (I). According tothe carotane sesquiterpene compound prepared in the invention, microalgae activity inhibition experiments prove that a median inhibitory concentration of the compound on the microalgae can reach 0.14microgram per milliliter.

Description

technical field [0001] The invention relates to the field of algae inhibitors, in particular to a carotane sesquiterpenoid derived from algae epiphyte fungi, a preparation method thereof, and an application in inhibiting microalgae. Background technique [0002] In the world, the red tide disasters in the coastal waters are becoming more and more frequent, which seriously hinders the development of marine fishery, coastal tourism and marine economy. After 2000, my country has entered a period of high incidence of red tides, and the frequency and harm of red tides have increased significantly. There are four main hazard forms of red tide, one is to destroy the marine ecological balance; the other is to destroy marine fishery resources; the third is to endanger human health; the fourth is to affect the development of marine tourism. According to statistics, the annual economic loss caused by red tides is as high as 1 billion yuan. There are about 300 species of microalgae th...

Claims

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Application Information

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IPC IPC(8): C07C49/733A01N35/06A01P13/00C12P7/38C12R1/885
CPCA01N35/06C07C49/733C07C2602/30C12P7/38
Inventor 季乃云时振振
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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