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Plant carotene synthesis relevant protein as well as coding gene and application thereof

A carotene and gene technology, applied in the direction of plant gene improvement, application, plant peptides, etc., can solve the problems of immature genetic transformation system and limitation of functional genomics research of leguminous plants.

Active Publication Date: 2018-08-14
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to factors such as large genomes of most legumes and immature genetic transformation systems, the study of functional genomics of legumes is limited.

Method used

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  • Plant carotene synthesis relevant protein as well as coding gene and application thereof
  • Plant carotene synthesis relevant protein as well as coding gene and application thereof
  • Plant carotene synthesis relevant protein as well as coding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, the discovery of WF1 and its coding gene WF1

[0059] 1. Mutant acquisition and phenotype analysis

[0060] By screening the Tnt1 insertion mutant library of Medicago truncatula, an extremely abnormal mutant whose flower color changed from wild-type yellow to white was obtained, named white flower1 (wf1).

[0061] See figure 1 . figure 1 Middle, A: wild-type R108 flower front; B: wild-type R108 flower petal; C: wild-type R108 flower fused wing petal and keel; D: wild-type R108 flower disomic stamen; E: wf1 flower front; F: wf1 flag petal; G: fused wing and keel petals of wf1 flower; H: disomic stamen of wf1 flower. In the wild-type R108, the flower front, flag petals, fused wing petals and keel are all yellow; the flower front, flower petals, flower fused wing petals and keel of wf1 are all white.

[0062] 2. Determination of anthocyanins

[0063] Plants to be tested: Medicago truncatula R108 (wild type) and wf1 mutant.

[0064] To analyze the pigm...

Embodiment 2

[0094] Example 2, Functional Verification of WF1 Gene

[0095] In order to further verify the role of WF1 in the regulation of carotenoid synthesis, functional verification was performed by performing WF1 genome complementation experiments in wf1 mutants.

[0096] 1. Construction of Complementary Expression Vector

[0097] The DNA fragment shown in sequence 3 of the sequence listing was inserted into the KpnI restriction site of the pCAMBIA2300 plasmid, and the DNA fragment shown in sequence 4 of the sequence listing was inserted into the PstI restriction site to obtain the recombinant plasmid pWF1pro::WF1gDNA -1.5KASC (confirmed by sequencing).

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Abstract

The invention discloses plant carotene synthesis relevant protein as well as a coding gene and application thereof. The invention provides a protein of (a1) or (a2): (a1) protein consisting of an amino acid sequence shown by a sequence 2 in a sequence table; (a2) protein obtained by performing amino acid residue substitution and / or deletion and / or addition on the amino acid sequence shown by the sequence 2 in the sequence table and derived from the sequence 2 having the same function. The inventor clones a WF1 gene from Medicago truncatula, successfully builds a plant supplementary carrier, and uses an agrobacterium-mediated leaf disc method for converting Medicago truncatula white flower mutants. Compared with a control group, the protein has the advantages that the white flower of the Medicago truncatula subjected to WF1 gene transplanting becomes yellow. The content disclosed by the invention can lay out foundation for the study of carotenoid synthesis regulation and control of thegene in plants.

Description

technical field [0001] The invention relates to a plant carotenoid synthesis-related protein, its coding gene and application. Background technique [0002] There are about 700 genera and 18,000 species of legumes (Leguminosae), which are the third largest family after Compositae and Orchidaceae. source, with important economic, ecological and biological value. However, due to factors such as large genomes of most legumes and immature genetic transformation systems, the study of functional genomics of legumes is limited. Medicagot truncatula (Medicago truncatula) is an annual herbaceous plant, grazing grass, because of its short growth cycle, small ploidy, small genome, self-pollination and nitrogen fixation, it is an ideal model plant for the study of legume genetics and genomics. [0003] Carotenoids are a general term for a class of C40 terpenoids and their derivatives. They are the basic components of all photosynthetic organisms and widely exist in animals, plants, al...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/02A01H6/54
CPCC07K14/415C12N15/8205C12N15/825
Inventor 林浩王作以孟颖颖牛丽芳刘欢
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI