A metal affinity adsorbent for the removal of proteins from serum and the detection of nucleosides in serum

An adsorbent and affinity technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that the sample cannot be directly introduced into the liquid phase for detection, the blockage of the chromatographic column, and the complicated process, etc., to achieve direct measurement, short equilibration time, The effect of simple preparation method

Active Publication Date: 2020-07-03
林甸县金源畜牧生物科技有限公司
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Problems solved by technology

When detecting small molecules in serum, the complex components in serum, especially some high-abundance proteins, will interfere with the detection of these substances, so that the sample cannot be directly tested in the liquid phase, and the chromatographic column will be tested jam
Therefore, the method commonly used by researchers is to selectively enrich the target and then detect it. This process needs to solve the selectivity, adsorption capacity and elution conditions, etc. The process is complicated and the cost is high.

Method used

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  • A metal affinity adsorbent for the removal of proteins from serum and the detection of nucleosides in serum
  • A metal affinity adsorbent for the removal of proteins from serum and the detection of nucleosides in serum
  • A metal affinity adsorbent for the removal of proteins from serum and the detection of nucleosides in serum

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Embodiment 1

[0024] Dissolve 3.0 g sodium alginate (SA-Na) in 97.0 g aqueous solution containing 6wt% sodium hydroxide and 4wt% urea to obtain a 3wt% sodium alginate solution, which is fully stirred to form a transparent solution; similarly, the 3.0 g sodium carboxymethylcellulose was dissolved in 97.0 g of an aqueous solution containing 6wt% sodium hydroxide and 4wt% urea to obtain a 3wt% sodium carboxymethylcellulose (CMC-Na) solution;

[0025] The above two solutions were mixed according to the mass ratio of sodium alginate: sodium carboxymethyl cellulose was 1:1, and mixed into a solution with a total mass of 10.0 g. cross-linked calcium chloride solution, stirred thoroughly for 20min, then suction filtered and fully washed with deionized water. The washed material was dried in a 60°C oven, and the SA-Ca / CMC-Ca material was manually ground after drying. The SA-Ca / CMC-Ca composite material can be obtained.

[0026] SA-Ca / CMC-Ca is white powder, insoluble in water.

[0027] Weigh 50.0 ...

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Abstract

The invention relates to a metal adsorbent for removing proteins in serum and directly detecting nucleosides in the serum. The proteins with naked histidine on the surface are adsorbed and removed byusing the adsorption effect of sodium alginate and sodium carboxymethylcellulose on copper ions and further using the complexation effect of the copper ions and histidine. The detection rate of the proteins can reach 80% or more. The metal adsorbent is used for removing proteins in the serum and directly detecting small molecules in the serum in practical application, and has the advantages of lowcost, simplicity in synthesis and high detection efficiency.

Description

technical field [0001] The invention relates to the technical field of functional material preparation and detection thereof, in particular to a metal-affinity adsorbent for removing protein in serum and detecting nucleosides in serum. Background technique [0002] Serum is a complex mixture formed from plasma depleted of fibrinogen. It is mainly composed of water and various chemical components. These chemical components include albumin, α1, α2, β, γ-globulin, triglyceride, total cholesterol, alanine aminotransferase and so on. When detecting small molecules in serum, the complex components in serum, especially some high-abundance proteins, will interfere with the detection of these substances, so that the sample cannot be directly tested in the liquid phase, and the chromatographic column will be tested clogged. Therefore, the commonly used method for researchers is to selectively enrich the target substance and then detect it. This process needs to solve the selectivit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/08
CPCG01N30/02G01N30/08
Inventor 张海霞王悦
Owner 林甸县金源畜牧生物科技有限公司
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