Method for enriching endocrine interfering substances by using silica self-assembled microcapsule
A technology of endocrine disruptors and silicon dioxide, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of long processing period, complex matrix interference, low concentration, etc., and achieve the goal of short time and good enrichment index Effect
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Embodiment 1
[0048] Embodiment 1 (product preparation):
[0049] A kind of silicon dioxide self-assembled microcapsule, its preparation steps are as follows:
[0050] (1) Preparation of amphiphilic silica particles:
[0051]Dissolve 0.60g CTAB and 0.20g polyoxyethylene lauryl ether Brij56 in 100ml of well-stirred buffer solution (0.16g of sodium hydroxide and 0.68g of sodium dihydrogen phosphate), heat up to 80°C, and when the solution is uniform, put 2.00mL of TEOS was added dropwise into the above surfactant solution, and stirred by magnetic force for 20h. After the reaction was completed, it was centrifuged, washed 5 times with ethanol, and vacuum-dried at 60° C. for 48 hours to obtain 80 mg of amphiphilic silica particles.
[0052] (2) Preparation of silica self-assembled microcapsules:
[0053] Take 5 mg of the amphiphilic silica particles obtained in step (1) and place them in eight test tubes respectively, add 2 ml of 10 mg / L BPA solution as the water phase, and fully ultrasonica...
Embodiment 2
[0058] Embodiment 2 (microcapsule morphology observation):
[0059] A self-assembled microcapsule of amphiphilic silicon dioxide, the preparation steps of which are as follows:
[0060] (1) Preparation of amphiphilic silica particles:
[0061] The preparation method is the same as step (1) of Example 1.
[0062] (2) Preparation of silica self-assembled microcapsules:
[0063] Take 5 mg of amphiphilic silica particles obtained in step (1) and place them in a test tube, add 2 ml of calcein solution with a concentration of 5 mmol / L, and fully ultrasonically disperse; then add 100 μl of dodecane to the test tube to prepare a concentration of 10 mmol / L of Nile red dye, placed in a tumbler and tumbled for 2 hours at a speed of 80rad / min.
[0064] Observing the microcapsule morphology formed in step (2) under a fluorescence microscope, obtained Figure 5 (a), found to be in the size range of 100 μm to 500 μm, by water-soluble calcein Figure 5 (b) and oil-soluble Nile Red Fig...
Embodiment 3
[0065] Embodiment 3 (simulation application experiment): the application experiment of low-concentration BPA enrichment simulation sewage in the microcapsule of silicon dioxide self-assembly
[0066] Take respectively 5 mg of the amphiphilic silica particles prepared by step (1) of Example 1 and place them in eight test tubes, add 2 ml of bisphenol A (BPA) solution with a concentration of 10 mg / L, and fully ultrasonically disperse them; then Add 100 μl of cyclohexane, n-hexane, n-heptane, dodecane, n-hexanol, n-octanol, oleic acid and olive oil to each of the eight test tubes, place them in a tumbler at a speed of 80 rad / min for 24 hours, sufficient enrichment to reach equilibrium.
[0067] The concentration of BPA in the organic phase and the residual water phase was detected by high performance liquid chromatography, and the enrichment effect was evaluated.
[0068] Image 6 It is a comparison chart of the enrichment effect of low-concentration BPA in simulated sewage by s...
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