Preparation method and application of a CD132 gene-deleted immunodeficiency animal model

A technology of immunodeficiency and animal models, applied in the field of animal genetic engineering, can solve the problems of affecting experimental results, impure background, long cycle, etc.
CN108467873BActive Publication Date: 2020-03-13BIOCYTOGEN JIANGSU CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BIOCYTOGEN JIANGSU CO LTD
Publication Date
2020-03-13

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Abstract

The invention discloses a preparation method and application of an immunodeficiency animal model obtained by obtaining sgRNA capable of specifically targeting CD132 gene and CD132 gene deletion based on CRISPR / Cas9 technology. The invention also protects a CRISPR / Cas9 system for specifically targeting knockout of the CD132 gene, a method for preparing an sgRNA plasmid for obtaining an animal model of immunodeficiency, a method for preparing an animal model of immunodeficiency, and related applications. The invention provides a tool mouse suitable for human cell or tissue transplantation and a new method for preparing a humanized animal model, which is beneficial to the research of related diseases and provides an effective technical means for the development of biomedical experiments.
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Description

technical field

[0001] This application belongs to the field of animal genetic engineering and relates to CRISPR / Cas9 technology, in particular to a method for obtaining an animal model of immunodeficiency based on CRISPR / Cas9 gene knockout technology and an sgRNA for specifically targeting the CD132 gene. Background technique

[0002] CRISPR / Cas9 is an emerging technology discovered in recent years that the RNA-guided Cas9 nuclease targets the target gene for editing. In the CRISPR / Cas9 system, crRNA (CRISPR-derived RNA) combines with tracrRNA (trans-activating RNA) to form a double-stranded RNA through the principle of DNA base pairing, and guides the Cas9 protein to cut the double-stranded RNA at the target site of the crRNA-guided DNA sequence. strand DNA. The CRISPR / Cas9 system can realize highly flexible and specific genome editing in eukaryotic cells. It is currently the most popular new-generation genome editing technology in the field of genome editing. At present,...

Claims

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