Method for cultivation of plants using metal nanoparticles and nutrient medium for its implementation

A technology of nano-particles and cultivation methods, which is applied in the biological and nano-fields of plant cultivation, can solve problems such as destroying cells, and achieve the effect of improving morphology

Active Publication Date: 2018-08-31
SHENZHOUSPACEBIOTECHGRP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, carbon nanotubes are known to penetrate the human body through th...

Method used

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  • Method for cultivation of plants using metal nanoparticles and nutrient medium for its implementation
  • Method for cultivation of plants using metal nanoparticles and nutrient medium for its implementation
  • Method for cultivation of plants using metal nanoparticles and nutrient medium for its implementation

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preparation example Construction

[0044] For the preparation of the nutrient medium according to the invention, any known formulation of a balanced nutrient medium (containing all organic and inorganic macronutrients and micronutrients essential for plants) can be used as a basis. Therefore, in order to prepare the nutrient medium according to the present invention, the iron salts, zinc salts or copper salts or these salts are replaced by charge-neutral iron nanoparticles, charge-neutral zinc nanoparticles or charge-neutral copper nanoparticles, or combinations thereof, respectively. A combination of salts was introduced into the medium while the other components of the nutrient medium used as the base remained unchanged.

[0045] In order to demonstrate the possibility of implementing the present invention, the agar Murashing-Skoog nutrient medium formulation (see Table 1) was used as the basis to prepare the nutrient medium composition according to the embodiment of the present invention.

[0046] In an embo...

Embodiment 1

[0099] Example 1: Effect of metal nanoparticles and chitosan in nutrient medium composition on germination of tomato seeds.

[0100] Seed germination was characterized by the value of the germination index, defined as the percentage of the number of germinated seeds relative to the total number of seeds taken. Seed germination index was determined on day 15 after initiation of plant culture.

[0101] Figures 2A-2DShown is the effect of metal nanoparticles instead of metal salts and chitosan supplementation (100 mg / L) in the nutrient medium composition on the germination of Venice tomato seeds.

[0102] Figure 2A It was shown that the germination index of tomato seeds increased by 13-27% (compared to the control group) on the nutrient medium containing iron nanoparticles at a concentration of 0.6-10.0 mg / L. Addition of chitosan to nutrient media containing iron nanoparticles showed an additional 23-33% increase in germination index.

[0103] Such as Figure 2B It was s...

Embodiment 2

[0106] Example 2: Effect of Metal Nanoparticles in Nutrient Medium Compositions on Morphological Indicators of Pepper and Tomato Plants ring.

[0107] The morphological indicators of the plants (germination length, root length and green part biomass yield) were measured from the 40th day after the start of the cultivation of the plants on the nutrient medium containing metal nanoparticles (under the above conditions). The results are expressed as percentage magnitudes between the indices obtained in the experiment (test group) and in the control group.

[0108] Figure 3A-3D The effect of metal nanoparticles in the nutrient medium composition on the root length of LJ-king pepper is shown.

[0109] From Figure 3A As can be seen in , compared to the control group, in the nutrient containing iron nanoparticles at concentrations of 0.06 mg / L, 0.3 mg / L and 3.0 mg / L instead of iron in ionic form (5.6 mg / L as metal) Cultivation of pepper on medium resulted in increases in roo...

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Abstract

A method for cultivation of plants is used in the field of bio- and nanotechnology in plant cultivation and can be used for production of high quality planting material of agricultural plants, for implementation of biotechnology research, to improve the quality of agricultural products. The method can be used in aeroponic and hydroponic technologies and also for the creation of life-support systems for astronauts under conditions of long space flight. The method for cultivation of plants comprises the seed germination and subsequent cultivation of plants on the aseptic agar nutrient medium containing a set of essential for plant growth organic and inorganic components, including iron, zinc and copper, wherein iron or zinc, or copper or a combination thereof is in the form of electro neutral metal nanoparticles. Additionally, the nutrient medium can include chitosan. The method allows to improve the seed germination and to enhance the physiological and morphological indices of plants, such as the root length and root activity, the content of chlorophyll in the leaves, the sprout length, and the green mass yield.

Description

technical field [0001] The invention relates to the field of biology and nanotechnology in plant cultivation, which can be used to produce high-quality crop planting materials to guide biotechnology research, thereby improving the quality of agricultural products. The invention can be used in aerosol culture and hydroponics, and can also be used to create a life support system for astronauts under long-term space flight conditions. Background technique [0002] The application of nanomaterials as plant protection agents and fertilizers helps to increase the resistance of plants to adverse weather conditions and diseases, and to improve the yield and quality of crops. Nanotechnology formulations (eg, NanoGro, GreenLift, AgBion, etc.) are known, but research continues in the field of development of new formulations and methods of using nanotechnology to increase the efficiency of agricultural product production. The latest progress in this field is especially summarized in th...

Claims

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Application Information

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IPC IPC(8): A01G22/00A01G31/00A01G24/10A01G24/30
CPCA01G22/00A01G24/10A01G31/00A01N59/16A01N59/20B82Y5/00A01G22/05
Inventor 赵辉刘敏陈瑜鹿金颖李华盛孙乔G·S·尼奇塔洛A·N·日加奇I·O·利普尼斯基A·A·雅克米托娃O·A·博格斯洛夫斯卡N·N·格卢先科
Owner SHENZHOUSPACEBIOTECHGRP
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