Extraction method of active substances in mulberry leaves and storage method of active substances in mulberry leaves

The technology of an active substance and a preservation method is applied in the field of extraction and preservation of mulberry leaf active substances, and can solve problems such as difficulty in maintaining the stability of the active substance, single active substance components, and difficulty in meeting the cost or safety of large-scale production, etc. To achieve low price, good economy and environmental protection, environmental friendly effect

Active Publication Date: 2018-09-07
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the new solvents that have been reported include supercritical fluids and ionic liquids, it is difficult to meet the requirements of large-scale production due to cost or safety issues, and the extracted active substance has a single component, making it difficult to maintain the stability of the active substance

Method used

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  • Extraction method of active substances in mulberry leaves and storage method of active substances in mulberry leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for extracting and preserving active substances of mulberry leaves, comprising the following steps:

[0026] Weigh choline chloride and glycerin, mix according to the molar ratio of 1:2, the total mass is 100g, add them to the round bottom flask in turn, put them in a water bath at 80°C and keep stirring for 0.5h to form a transparent liquid. Add the synthesized solvent to 100g of water, weigh 50g of mulberry leaf powder and add it to the above-prepared solvent, extract in a 60°C water bath with ultrasonication at 150W for 0.5h, after ultrasonication, centrifuge the mixture at 12000rpm / min for 10min, and take the supernatant for detection and analysis , the measured yield of mulberry polyphenols in the extract was 206.2 mg / g, the yield of flavonoids was 73.9 mg / g, the yield of polysaccharides was 42.3 mg / g, and the yield of alkaloids was 4.5 mg / g. The extraction mixture was stored at 25° C., 4° C. and -20° C., and the content of the above-mentioned active subst...

Embodiment 2

[0028] A method for extracting and preserving active substances of mulberry leaves, comprising the following steps:

[0029] Weigh choline chloride and glucose, mix according to the molar ratio of 1:1, the total mass is 90g, add them to the round bottom flask in sequence, put them in a water bath at 80°C and keep stirring for 1 hour, and then form a transparent liquid. Add the synthesized solvent to 10g of water, weigh 30g of mulberry leaf powder and add it to the above-prepared solvent, extract it in a 60°C water bath with ultrasonication at 150W for 0.5h, after ultrasonication, centrifuge the mixture at 12000rpm / min for 10min, and take the supernatant for detection and analysis , the measured yield of mulberry polyphenols in the extract was 186.2 mg / g, the yield of flavonoids was 63.4 mg / g, the yield of polysaccharides was 35.6 mg / g, and the yield of alkaloids was 4.1 mg / g. The extraction mixture was stored at 25° C., 4° C. and -20° C., and the content of the above-mentioned...

Embodiment 3

[0031] A method for extracting and preserving active substances of mulberry leaves, comprising the following steps:

[0032] Weigh choline chloride and trehalose, mix according to the molar ratio of 2:1, the total mass is 100g, add them to the round bottom flask in turn, put them in a water bath at 80°C and keep stirring for 3 hours, and then form a transparent liquid. Add the synthesized solvent to 50g of water, weigh 30g of mulberry leaf powder and add it to the above-prepared solvent, extract it in a 60°C water bath with ultrasonication at 150W for 0.5h, after ultrasonication, centrifuge the mixture at 12000rpm / min for 10min, and take the supernatant for detection and analysis , the measured yield of mulberry leaf polyphenols in the extract was 253.2 mg / g, the yield of flavonoids was 92.9 mg / g, the yield of polysaccharides was 55.3 mg / g, and the yield of alkaloids was 5.6 mg / g. The extraction mixture was stored at 25° C., 4° C. and -20° C., and the content of the above-ment...

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Abstract

The invention discloses an extraction method of active substances in mulberry leaves and a storage method of active substances in mulberry leaves. The extraction method of the active substances in mulberry leaves comprises the following steps of mixing a natural low-eutectic solvent with water so as to obtain an extraction solvent; adding mulberry leaves, and carrying out heated extraction at 60-90 DEG C for 0.5-3.0 hours; and carrying out centrifugation so as to obtain liquid supernatant, wherein the liquid supernatant contains active substances, including polyphenols, flavones, polysaccharides, alkaloid and the like. Moreover, the active substances are stored in the liquid supernatant containing the natural low-eutectic solvent so that stability of the active substances is greatly improved. According to the extraction method disclosed by the invention, the natural low-eutectic solvent of plant-source components is used as an extraction solvent and a stabilization assistant so as to be subjected to active substance extract; and thus, the extracted active substances are broad in spectrum, and the prepared product is natural and safe in property. Moreover, interaction between hydrogen bonds of the natural low-eutectic solvent is also utilized so as to improve stability of the active substances; and thus, the extraction method is significantly superior than the prior art.

Description

technical field [0001] The invention relates to a method for extracting and preserving active substances of mulberry leaves. Background technique [0002] Organic solvents are generally used as extractants to obtain active ingredients from animals, plants or microorganisms. Due to the polar nature of organic solvents, it is difficult to maintain the stability of broad-spectrum active substances. At the same time, problems such as organic solvents are volatile, toxic, and solvent residues lead to technical bottlenecks in large-scale production. With the improvement of people's food and drug safety awareness, the use of traditional organic solvents in production has been controversial, and the development of new safe and green solvents is an inevitable trend to solve this urgent problem. [0003] Although the new solvents that have been reported include supercritical fluids and ionic liquids, it is difficult to meet the requirements of large-scale production due to cost or sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L3/36
CPCA23L3/36A23V2002/00A23L33/105A23V2250/21
Inventor 周鹏飞邝哲师王旭苹唐翠明戴凡炜潘木水王振江黄静
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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