Escherichia coli fermentation liquor concentration process and system

A technology of Escherichia coli and concentration system, which is applied in bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, bacteria, etc., can solve the problems of low density of concentrated bacteria and high accumulation of organic acids

Inactive Publication Date: 2018-09-07
HANGZHOU RUINA MEMBRANE ENG
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  • Abstract
  • Description
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Problems solved by technology

[0003] Aiming at the deficiencies of the prior art, the present invention provides a process and system for concentrating Escherichia coli fermentation br

Method used

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  • Escherichia coli fermentation liquor concentration process and system

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Embodiment Construction

[0012] Such as figure 1 Shown, a kind of Escherichia coli fermented liquid concentrating process, comprises the following steps: carry out diafiltration to Escherichia coli fermented liquid, then the filtrate is carried out concentrating and separating by ceramic membrane under operating pressure under 0-6Bar condition; Also includes the ceramic membrane Cleaning steps.

[0013] The ceramic membrane is made of silicon carbide or aluminum oxide; the pore size of the ceramic membrane is 200nm.

[0014] A system for concentrating Escherichia coli fermentation liquid, comprising a raw liquid tank 1, a delivery pump 2, a self-cleaning filter 3, and a ceramic membrane module 4 connected in series in sequence, and the ceramic membrane module 4 is connected to a concentrated solution tank 5 and a permeate tank 6 ; The operating pressure of the ceramic membrane module 4 is 0-6 Bar.

[0015] It also includes an online cleaning device, which includes a cleaning medicine box 7, a flushi...

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Abstract

The invention discloses an escherichia coli fermentation liquor concentration process, comprising the following steps: performing filter washing on escherichia coli fermentation liquor, and then performing concentrating and separating on the filtrate by a ceramic film under the condition of operation pressure of 0-6Bar. The invention also discloses an escherichia coli fermentation liquor concentration system, comprising a stock solution tank, a delivery pump, a self-cleaning filter and a ceramic film component which are connected in series, wherein the ceramic film component is internally connected with a concentrated liquor tank and a permeating liquor tank; and the operation pressure of the ceramic film component is 0-6Bar.

Description

technical field [0001] The invention relates to the technical field of concentrating Escherichia coli fermentation liquid, in particular to a process and system for concentrating Escherichia coli fermentation liquid. Background technique [0002] During the fermentation process of Escherichia coli, the glucose uptake rate and the cell's carbon metabolism rate are often unbalanced, resulting in excessive organic acid production, slow bacterial growth and other adverse metabolic consequences. At the same time, the bacterial concentration of Escherichia coli strains is generally low, which is not conducive to large-scale high-density fermentation in industrial production. Therefore, increasing the cell density of cell fermentation and reducing the accumulation of organic acids is the key to improving the growth characteristics of E. coli. Contents of the invention [0003] Aiming at the deficiencies of the prior art, the present invention provides a process and system for co...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12M1/12C12M1/00C12R1/19
CPCC12N1/20C12M47/02C12N1/02
Inventor 陈幸培陈健陈睿东雷园园陈建滨
Owner HANGZHOU RUINA MEMBRANE ENG
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