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Use of linc01836 in the preparation of gastric cancer diagnostic products and therapeutic drugs

A gastric cancer and product technology, applied in the field of biomedicine, can solve the problems of non-conservation of lncRNA, diagnosis and gene therapy impact, etc., to achieve the effect of improving early diagnosis and reducing mortality

Active Publication Date: 2019-12-31
BEIJING MEDINTELL BIOMED CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of studies have shown that lncRNA is extremely unconserved in biological evolution, and its expression specificity in tissue and developmental stages is significantly higher than that of protein-coding genes. The possible side effects of gene intervention therapy targeting tumor-associated lncRNA will be less than those of protein-coding genes. Encoding gene targets, the discovery of tumor-associated lncRNA may have a significant impact on tumor diagnosis and gene therapy

Method used

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  • Use of linc01836 in the preparation of gastric cancer diagnostic products and therapeutic drugs
  • Use of linc01836 in the preparation of gastric cancer diagnostic products and therapeutic drugs
  • Use of linc01836 in the preparation of gastric cancer diagnostic products and therapeutic drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Screening of differentially expressed non-long-chain coding RNA

[0052] 1. Research object:

[0053] Surgical specimens (gastric cancer tissues and corresponding paracancerous tissues) of 5 patients with primary gastric cancer who underwent radical gastrectomy in the Department of Oncology of the hospital were collected, and each patient signed an informed consent.

[0054] Inclusion criteria: a. Preoperative diagnosis of primary gastric cancer, and no tumor treatment; b. No other malignant tumors; c. No other complications, such as gastric perforation, gastrointestinal bleeding, gastrointestinal obstruction, etc. The general condition of the patient was good before operation; d. No other chronic diseases, such as hypertension, diabetes, etc.

[0055] This study was approved by the ethics committee.

[0056] 2. Sample acquisition

[0057] 1) After the surgical sample is isolated from the tumor, the tumor sample is selected, with a size of about 0.5cm x 0.5...

Embodiment 2

[0085] Example 2 Large sample verification screened out differentially expressed LncRNA

[0086] Based on the screening results of Example 1 and according to the size of P value, LINC01836 was selected for verification.

[0087] 1. Sample collection

[0088] According to the method of Example 1, 45 gastric cancer tissues and 45 corresponding paracancerous tissues were collected.

[0089] 2. Validation at the transcript level

[0090] Reagents: Reverse transcription kit (DDR037A) was purchased from Treasure Bioengineering (Dalian) Co., Ltd. SYBR Premix Ex Taq for real-time quantitative PCR (polymerase chain reaction) TM (Tli RNaseHPlus) kit was produced by Japan Takara Company.

[0091] 2.1 Extract tissue RNA

[0092] Step is with embodiment 1.

[0093] 2.2 Primer design

[0094] According to the LINC01836 transcript sequence, primers were designed by NCBI's primer design tool (Primer BLAST), upstream primer: 5'-TGAAGAAACCGTGGAAAC-3' (SEQ ID NO.2); downstream primer: 5'-...

Embodiment 3

[0102] Embodiment 3 inhibits the expression of LINC01836

[0103] 1. Cell culture and transfection

[0104] Cell culture: Gastric cancer cell line BGC-823 was used in DMEM containing 10% FBS and placed in 5% CO 2 , Saturated humidity, cultured in a 37°C carbon dioxide incubator. The culture medium was changed every two days, and the cells were digested with 0.25% trypsin when subcultured.

[0105] siRNA transfection: The day before transfection, cells are digested and inoculated into culture dishes or culture plates, and the number of cells inoculated should ensure that the density of 30-50% can be reached on the second day of transfection. siRNA transfection strictly according to Lipofectamin TM 2000 instructions, after 4-6 hours, replace with fresh culture medium containing 10% FBS, and continue to cultivate for 48-72 hours.

[0106] 2. siRNA design

[0107] Design of siRNA (small interfering RNA): siRNA sequence was designed in the specific sequence region of LINC01836...

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Abstract

The invention relates to detection of a long chain non-coding RNA used for gastric cancer screening and an application of the long chain non-coding RNA. According to the invention, LINC 01836 which isobviously and highly expressed in gastric cancer tissues is screened by utilizing a chip screening technology. Compared with para-carcinoma tissues, LINC01836 is obviously and highly expressed in thegastric cancer tissues, and the conclusions are further verified in a large sample fluorescent quantitative PCR experiment. LINC01836 has correlation with gastric cancer, study of gastric cancer pathogenesis can be further enriched, and a new tumor marker and a new therapeutic target are also provided for early diagnosis of gastric cancer as well as prognosis monitoring.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the use of LINC01836 in preparing gastric cancer diagnostic products and therapeutic drugs. Background technique [0002] Gastric cancer is one of the most common malignant tumors, and its morbidity and mortality vary greatly in different regions and different groups of people. The incidence and mortality of gastric cancer in my country are the highest in the east and northwest. , Ningxia, Inner Mongolia, Liaoning, and then along the coast to Jiaodong Peninsula and Jiangsu and Zhejiang are high-incidence areas of gastric cancer in my country (Chen Wanqing, Zhang Siwei, Chen Zhifeng. Analysis of the epidemic trend of gastric cancer in high-incidence areas of esophageal cancer and gastric cancer in China[[J]. China Oncology, 2008 (12):998-1000). At the same time, geographical epidemiological studies of gastric cancer also suggest that there are significant differences in the geographical dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12Q1/6886A61K45/00A61K31/7088A61P35/00
CPCA61K31/7088A61K45/00A61P35/00C12N15/113C12N2310/14C12Q1/6886C12Q2600/158C12Q2600/178
Inventor 吴东杨承刚郭涛张改英陈丽媛
Owner BEIJING MEDINTELL BIOMED CO LTD
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