Detection method for lysozymes in egg white of plateau specially bred rare bird egg

Abstract

The invention discloses a detection method for lysozymes in egg white of a plateau specially bred rare bird egg and relates to the technical field of lysozyme analysis and detection. The detection method comprises the following steps: (a) establishing a lysozyme standard curve: dissolving and diluting a lysozyme standard solution into different concentration gradients by adopting a 0.05 to 0.08mol / L phosphate buffer solution with pH of 6.0 to 6.5, and measuring an absorbance value at a wavelength of 279 to 283nm by use of ultraviolet spectrophotometry; (b) detecting a sample: diluting a rare bird egg white sample to be detected with the phosphate buffer solution, performing analysis in a manner the same as that for a standard, measuring an absorbance value of the sample to be detected andcalculating the lysozyme content of the rare bird egg white sample according to the absorbance value by use of the standard curve. According to the detection method, a low-concentration phosphate buffer solution system is adopted, so that a lysozyme measurement result is more accurate, and the detection method is higher in precision and recovery rate, high in sensitivity and applicable to measurement of the lysozymes in the plateau specially bred rare bird egg.

Description

technical field [0001] The invention relates to the technical field of analysis and detection of lysozyme, in particular to a method for detecting lysozyme in the egg white of rare poultry eggs from plateau special breeding. Background technique [0002] Lysozyme, also known as muramidase or N-acetylmuramide glycanohydrlase, is an alkaline enzyme that can hydrolyze mucopolysaccharides in pathogenic bacteria. Lysozyme mainly breaks the β-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in the cell wall to decompose the insoluble mucopolysaccharide in the cell wall into soluble glycopeptides, resulting in the release of the cell wall contents to dissolve the bacteria. Lysozyme can also directly combine with negatively charged viral proteins, and form double salts with DNA, RNA, and apoproteins to inactivate the virus. Therefore, the enzyme has antibacterial, anti-inflammatory, antiviral and other effects. [0003] Lysozyme is widely found in various ...

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Problems solved by technology

[0006] The traditional method uses 0.9% sodium chloride as the dilution solution of the system. If the salt concentration is too high, it will easily affect the accuracy of the determination of lysozyme content. In the later stage, the pH of the system needs to be adjusted, and the operation is cumbersome, resulting in inaccurate measurement results.

At the same time, this method is a universal method, but the lysozyme content in the rare poultry eggs specially bred in plateau areas is much higher than that of conventional eggs. If this method is used to measure the results, it is easy to cause inaccurate results. At present, there is no one that is fully applicable. Detection method of lysozyme in plateau special breeding rare poultry eggs

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