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Detection method and detection device for methylglyoxal content in honey

The technology of a detection method and detection device, which is applied in the field of bee breeding, can solve the problems of unsuitable promotion, high detection cost, and long time-consuming detection process, and achieve the effects of good repeatability and stability, high sensitivity, and low detection limit

Active Publication Date: 2018-09-14
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the specific standard procedures and complicated sample pretreatment process of these methods, the detection process takes too long. In addition, because the above-mentioned technologies must be used with expensive instruments, such as various chromatographs and mass spectrometers, etc., Its detection cost is too high, not suitable for promotion

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  • Detection method and detection device for methylglyoxal content in honey
  • Detection method and detection device for methylglyoxal content in honey
  • Detection method and detection device for methylglyoxal content in honey

Examples

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Embodiment 1

[0042] Such as figure 1 Shown, the detection method of methylglyoxal content in the honey of the present invention comprises the steps:

[0043] S1, prepare detection electrode, in the present embodiment, described detection electrode is a three-electrode system, and it comprises: the glassy carbon electrode (GCE) as working electrode, the saturated KCl calomel electrode (SCE) as reference electrode and as auxiliary The platinum wire (Pt) of electrode; Specifically, described step S1 comprises:

[0044] S11. Cover the bare electrode surface of the glassy carbon electrode with 0.05 μm Al on the suede 2 o 3 The grinding powder is ground until smooth, then rinsed with deionized water;

[0045] S12. Use deionized distilled water, absolute ethanol, and deionized distilled water to perform ultrasonic cleaning on the bare electrode obtained in step S11. Each treatment process takes 4-10 minutes (preferably 5 minutes), and then at room temperature dry;

[0046] S2, using carbon n...

Embodiment 2

[0055] The only difference between this example and Example 1 is that in step S21, 0.15 mg mL -1 Carboxymethylcellulose solution and 0.8mg mL -1 Carboxymethylcellulose-multi-walled carbon nanotube (CMC-fMWCNT) suspension; in step S22, prepare 0.15% chitosan (CS) solution; in step S23, prepare mixed droplets in 3 μL step S22 on the glassy carbon electrode and dried; in step S31, adjust the pH of the diluted honey sample solution to 9 with NaOH solution.

Embodiment 3

[0057] The only difference between this example and Example 1 is that in step S21, 0.45mg mL -1 Carboxymethylcellulose solution and 1.2mg mL -1 Carboxymethyl cellulose-multi-walled carbon nanotube (CMC-fMWCNT) suspension; in step S22, prepare 0.25% chitosan (CS) solution; in step S23, prepare mixed droplets in 8 μL step S22 on the glassy carbon electrode and dried; in step S31, adjust the pH of the diluted honey sample solution to 11 with NaOH solution.

[0058] Carbon nanotubes (CNTs), as one of the common carbon nanomaterials, have good electrical, chemical, mechanical and structural properties, and can be used to modify electrodes very well. Owing to their many excellent properties, such as high electrical conductivity, large active surface area, easy-to-change surface, catalytic activity, excellent chemical stability, and good biocompatibility, they have been widely used in electrochemical sensors nowadays.

[0059] Carboxymethylcellulose (CMC) is an important cellulose ...

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Abstract

The invention discloses a detection method and a detection device for the methylglyoxal content in honey. The detection method includes S1, arranging a detection electrode; S2, modifying the detectionelectrode by a carbon nanotube; S3, detecting the honey by the detection electrode modified by the carbon nanotube to acquire methylglyoxal content data in the honey. According to the detection method and the detection device, an electrochemical method is used for detecting the methylglyoxal content in the honey, and the glassy carbon electrode modified by the multi-wall carbon nanotube functionalized by carboxyl is mainly used for detecting the methylglyoxal content in the honey. The detection method and the detection device have the advantages of quickness in analysis, low cost, simplicityand convenience in operation, high sensitivity, stability and selectivity, and the like.

Description

technical field [0001] The invention relates to the field of bee breeding, in particular to a detection method and a detection device for the content of methylglyoxal in honey. Background technique [0002] There is a kind of Manuka honey produced in New Zealand on the market, which has strong antibacterial activity, and one of its active substances is methylglyoxal. Since the application of methylglyoxal is worthy of further exploration, a lot of research has been done on the determination of the content of methylglyoxal. Common techniques are such as high performance liquid chromatography, ion-pair chromatography and other chromatographic techniques, as well as mass spectrometry, capillary electrophoresis testing methods, etc. detection. However, due to the specific standard procedures and complicated sample pretreatment process of these methods, the detection process takes too long. In addition, because the above-mentioned technologies must be used with expensive instrum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 吴小波张文娟文阳平曾志将
Owner JIANGXI AGRICULTURAL UNIVERSITY
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