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Trichoderma longibrachiatum strain and application thereof

The technology of Trichoderma longbranchi and Trichoderma longbranchi, which is applied in the field of microorganisms, can solve the problems of soil cadmium pollution, lowering the immune function of the body, and high repair costs, and achieves the improvement of soil organic matter content, improvement of soil physical and chemical properties, and significant resistance to diseases and insect pests. Effect

Active Publication Date: 2018-09-28
武汉合缘绿色生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, heavy metal cadmium can cause chronic poisoning, carcinogenicity, teratogenicity, and lower immune function after entering the human body, especially the "cadmium rice" poisoning incident that has occurred in recent years, which has made the restoration of soil cadmium pollution urgently needed to be solved.
Both physical restoration and chemical restoration are easy to cause secondary pollution, and the repair cost is high

Method used

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  • Trichoderma longibrachiatum strain and application thereof
  • Trichoderma longibrachiatum strain and application thereof
  • Trichoderma longibrachiatum strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the screening of Trichoderma longibrachiata T61

[0023] 1. Primary screening

[0024] Take 10g of soil samples from an abandoned factory producing cadmium, put them into a triangular flask containing 90mL of sterilized liquid PDA medium under aseptic conditions, place them on a shaker at 200r / min at 28°C for 12h; draw 0.1mL of bacterial solution to apply Cloth on Cd 2+ On the PAD solid plate medium with a concentration of 50 mg / L, it was placed in a 28°C incubator and cultured upside down for 3 days, and repeated 3 times.

[0025] 2. Re-screening

[0026] The colonies grown on the above plates were streaked and inoculated on Cd 2+ On the PAD solid plate medium with a concentration of 50mg / L, 100mg / L, 200mg / L, 300mg / L, 400mg / L and 500mg / L, it was cultivated upside down in a 30°C incubator for 3 days, and repeated 3 times. Observe the growth of the colony, and store the solid plate with the growth of the colony in a refrigerator at 4°C.

[0027] A tota...

Embodiment example 2

[0034] Implementation case 2: Classification and identification of strain T61

[0035] 1. Morphological Identification

[0036] From figure 2 It can be seen that when the T61 strain was cultivated on the PAD plate for 4 days, the colony diameter reached 85mm, the colony was round and flat, and the initial white hyphae turned dark green later, the mycelium was dense and tapered, and the back of the colony was colorless. Hyphae are transparent with septa, conidiophores are long and straight, 5.0-20.0 μm; secondary branches grow at right angles, some are slightly curved towards the main branch; terminal small stalks are bottle-shaped, solitary, opposite or 2- 5 whorls, stalks are thin at the base, enlarged in the middle, and slender upward to the top; conidia are obovate, elliptic, light green, smooth, about 2.0-2.5 μm × 2.5-5.0 μm.

[0037] 2. Molecular biological identification

[0038] Pick 0.5 g of mycelium of T61 strain into a mortar, add liquid nitrogen and quickly grin...

Embodiment example 3

[0045] Implementation Case 3: Biological verification of the degradation of cadmium by Trichoderma longibrachiata

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Abstract

The invention discloses a trichoderma longibrachiatum strain and an application thereof. The trichoderma longibrachiatum T61 provided by the invention has cadmium reducing and bio-control functions; the strain is preserved in China Center for Type Culture Collection with preservation number of CCTCC NO: M2017447, and nucleotide sequence of a 28Sr RNA gene is shown as SEQ ID No.1. Based upon experiments, it is proved that the trichoderma longibrachiatum T61 provided by the invention can purify cadmium of an exchangeable form in soil so that the content of the cadmium is obviously reduced; the trichoderma longibrachiatum T61 is quite strong in tolerance to cadmium, low in disturbance to soil and relatively strong in bioremediation function; meanwhile, crops, applied by the trichoderma longibrachiatum T61, are obvious in effects of resisting diseases and pests, and meanwhile, biomass content in the soil can be obviously improved and physiochemical properties of the soil can be improved. Therefore, the trichoderma longibrachiatum T61 provided by the invention can be applied to remediation of heavy metal contaminated soil, improvement of the biomass content of the soil, improvement of the physiochemical properties of the soil as well as to preparation of related products.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Trichoderma longibrachiata and an application thereof. Background technique [0002] Cadmium is a relatively rare metal, a heavy metal element with strong toxicity, wide distribution and long half-life. The main sources of cadmium in soil are industrial waste residues, diffusion in waste gas, sedimentation, accumulation, irrigation of farmland with wastewater, and extensive use of pesticides and phosphate fertilizers. It easily accumulates in the soil. In the current heavy metal pollution, cadmium pollution is very prominent, and it is one of the most extensive and harmful heavy metal elements. According to statistics, the area of ​​cultivated land polluted by cadmium in my country is 20 million square meters, accounting for 20% of the total area of ​​cultivated land in my country, and the amount of food polluted by heavy metals is as high as 12 million tons ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G13/00B09C1/10C12R1/885
CPCA01G13/00B09C1/105C12N1/14C12N1/145C12R2001/885
Inventor 杨涛徐辉徐维烈胡定志袁庆丰
Owner 武汉合缘绿色生物股份有限公司
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