A kind of Trichoderma longibrachiata strain and application thereof
A technology of Trichoderma longibranchus and Trichoderma longibranchus, applied in the field of microorganisms, can solve the problems of soil cadmium pollution, lower immune function of the body, high repair cost, etc., to increase soil organic matter content, improve soil physical and chemical properties, and significantly resist diseases and insect pests Effect
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Embodiment 1
[0022] Embodiment 1: the screening of Trichoderma longibrachiata T61
[0023] 1. Primary screening
[0024] Take 10g of soil samples from an abandoned factory producing cadmium, put them into a triangular flask containing 90mL of sterilized liquid PDA medium under aseptic conditions, place them on a shaker at 200r / min at 28°C for 12h; draw 0.1mL of bacterial solution to apply Cloth on Cd 2+ On the PAD solid plate medium with a concentration of 50 mg / L, it was placed in a 28°C incubator and cultured upside down for 3 days, and repeated 3 times.
[0025] 2. Re-screening
[0026] The colonies grown on the above plates were streaked and inoculated on Cd 2+ On the PAD solid plate medium with a concentration of 50mg / L, 100mg / L, 200mg / L, 300mg / L, 400mg / L and 500mg / L, it was cultivated upside down in a 30°C incubator for 3 days, and repeated 3 times. Observe the growth of the colony, and store the solid plate with the growth of the colony in a refrigerator at 4°C.
[0027] A tota...
Embodiment example 2
[0034] Implementation case 2: Classification and identification of strain T61
[0035] 1. Morphological Identification
[0036] From figure 2 It can be seen that when the T61 strain was cultivated on the PAD plate for 4 days, the colony diameter reached 85mm, the colony was round and flat, and the initial white hyphae turned dark green later, the mycelium was dense and tapered, and the back of the colony was colorless. Hyphae are transparent with septa, conidiophores are long and straight, 5.0-20.0 μm; secondary branches grow at right angles, some are slightly curved towards the main branch; terminal small stalks are bottle-shaped, solitary, opposite or 2- 5 whorls, stalks are thin at the base, enlarged in the middle, and slender upward to the top; conidia are obovate, elliptic, light green, smooth, about 2.0-2.5 μm × 2.5-5.0 μm.
[0037] 2. Molecular biological identification
[0038] Pick 0.5 g of mycelium of T61 strain into a mortar, add liquid nitrogen and quickly grin...
Embodiment example 3
[0045] Implementation Case 3: Biological verification of the degradation of cadmium by Trichoderma longibrachiata
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