A rapid method for identifying sex-specific molecular markers and hereditary sex in rusty spot moth

Abstract

The invention relates to a method for quickly identifying sex-specific molecular markers and hereditary sex of rusty spot moth, and the SNP site used for sex identification of rust spot moth. The base sequence is: SEQ ID NO: 1 or SEQ ID NO: 2, length It is 368bp; the SNP site is: 35th, 89th, 120th, 218th, 240th in SEQ ID NO: 1 or SEQ ID NO: 2. Identification methods include: extraction of DNA from male and female rusty spotters of known gender; construction of a simplified genome library and high-throughput sequencing; sequence analysis and screening of sex-specific SNP sites; expanded sample verification of candidate SNP sites; The design of sex-specific primers for the point; use male-specific primers at a specific annealing temperature to carry out PCR amplification on the genomic DNA of P. russica, and determine the genetic sex according to the results of agarose gel electrophoresis. The method can provide a reliable method for the identification of the genetic sex of the rusty spot, especially the early stage of the development of the rusty spot, and has the advantages of simple operation, short time consumption, low cost, good repeatability, and high accuracy.

Description

technical field [0001] The invention belongs to the marine crab sex identification technology in the technical field of aquatic organisms, and in particular relates to a method for quickly identifying sex-specific molecular markers and genetic sex of rusty spot moth. Background technique [0002] SNP (Single Nucleotide Polymorphism) refers to the variation of a single nucleotide on the genome, including transitions, transversions, deletions, and insertions, forming genetic markers with a large number and rich polymorphisms. As the third generation genetic molecular marker, SNP has been widely used in the research of genetic diversity analysis, gene mapping, molecular marker-assisted breeding and functional genomics of many species. [0003] Charybdis feriatus, commonly known as safflower crab, belongs to the class Crustacea, order Decapoda, family Portunidae, genus Charybdis, and is mainly distributed in the tropical, subtropical and temperate regions of the Pacific Ocean an...

AI Technical Summary

Problems solved by technology

However, due to the fact that the technical means of screening polymorphic genetic markers through simplified genome sequencing is not widely used, and there is no combination of molecular methods for identifying the sex of rusty spot. Now, in production practice or academic research, only morphological methods can be used to identify rusty spot. Gender, the identification accuracy is low, and it is difficult to identify the sex of the rusty spot in the early stage of growth and development. There are no reports on sex-linked markers and genetic sex identification methods with the rusty spot.

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