Degradation bacteria for sulfonamide antibiotics and application thereof

A technology of sulfonamides and antibiotics, applied in the field of environmental pollutant treatment, can solve problems such as antibiotic pollution, achieve the effects of reducing harm, good degradation effect, and broad application prospects

Active Publication Date: 2018-10-02
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It aims to promote the application of microbial technology in environmental sulfa antibiotic pollution control and solve the problem of environmental sulfa antibiotic pollution

Method used

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  • Degradation bacteria for sulfonamide antibiotics and application thereof
  • Degradation bacteria for sulfonamide antibiotics and application thereof
  • Degradation bacteria for sulfonamide antibiotics and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and identification of sulfonamide antibiotic-degrading bacteria

[0030] 1. Experimental materials

[0031] Nutrient medium: 5 g sodium chloride, 3 g beef extract, 10 g protein, 1000 mL distilled water, pH 7.0-7.2. For solid medium, add agar powder (bar) 15 g / L. Inorganic salt medium: (NH 4 ) 2 SO 4 1g, MgSO 4 ·7H 2 O 0.1g, KH 2 PO 4 3g,K 2 HPO 4 ·3H 2 O 7g, sodium citrate 0.5g, distilled water 1000mL, pH 7.0-7.2.

[0032] Before the experiment, the medium was placed in a high-pressure steam sterilizer, and sterilized at 121° C. for 30 minutes.

[0033] 2. Isolation and identification of strains

[0034] (1) Screening and domestication of sulfa antibiotic-resistant bacteria:

[0035] Microbial domestication proceeds in four stages. To collect contaminated soil samples from a livestock and poultry farm in the Pearl River Delta, first add the soil samples to 100 mL of nutrient medium containing sulfonamide antibiotics (10 mg / L) under a...

Embodiment 2

[0046] The mensuration of the growth curve of embodiment 2 yeast A5

[0047] 1. Inoculate yeast A5 into LB medium and LB medium containing sulfonamide antibiotics respectively, place them on a shaker at 25 °C and 150 r / min at constant temperature, and culture them at 0.5, 1, 1.5, 2, 3, 4, 6, 8, 10, 12, 24, 36, 48, 60, 72, 96, 120, 144, 168, 192, 216 h sampling, determination of culture medium OD 600 , zeroed with sterilized LB liquid medium.

[0048] The LB medium is: NaCl 5g / L, yeast extract 10g / L, trypsin 10g / L, water 1000mL, pH 7.0-7.2.

[0049] The LB medium containing sulfa antibiotics is as follows: adding sulfamethazine to the above LB medium to make the concentration 100 mg / L.

[0050] 2. The result is as follows Figure 4 As shown, the yeast A5 can also grow normally in the culture system containing sulfa antibiotics.

Embodiment 3

[0051] Example 3 Saccharomyces Degradation Experiment of Saccharomyces A5

[0052] 1. Experimental method

[0053] (1) Preparation of bacterial suspension:

[0054] Scrape 1~2 loops of bacteria A5 from the seed preservation slope, insert them into sterilized LB medium, wrap them up and place them in a constant temperature shaker incubator (25°C, 150 r / min), and expand the culture for 24~36 h. Take the culture solution of activated bacteria A5, centrifuge (6000 r / min, 25°C), collect the bacteria, wash twice with sterile water (6000 r / min, 25°C), and then resuspend with sterile water to prepare A certain concentration of bacterial suspension.

[0055] (2) Degradation of Sulfamethazine by Saccharomyces A5:

[0056] A certain amount of sulfamethazine was added to the high-pressure sterilized inorganic salt medium as the sole carbon source to make the concentration 1 mg / L; then 1 mL of bacterial suspension was added to make the bacterial dosage 1 g / L (wet weight); at the same ...

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Abstract

The invention discloses a degradation bacteria for sulfonamide antibiotics and an application thereof, wherein the bacteria is Sakaguchia cladiensis A5 and is preserved in Guangdong Center of Microbial Culture Collection at Jan. 26th, 2018, and is assigned the accession number of GDMCC No.60319. The bacterial strain has great degradation effect on sulfonamide antibiotics such as sulfamerazine, sulfamethoxazole and the like, and can be used for degradation treatment to sulfonamide antibiotics pollution to application objects comprising wastewater, waste residue, water environment, soil and thelike, which contain the sulfonamide antibiotics. The bacterial strain can reduce damage on ecological environmental safety due to the antibiotics, is energy-saving and environment-friendly, and has extensive application prospect.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant treatment. More specifically, it relates to a sulfonamide antibiotic degrading bacterium and its application. Background technique [0002] my country is a big country in the production and use of antibiotics. Thousands of tons of antibiotics are used in poultry farming and personal medical care every year. Among them, sulfonamide antibiotics have become my country's pharmaceutical and veterinary antibiotics due to their broad-spectrum, high quality and low price advantages. The most widely used category. In fact, most antibiotics are not fully absorbed by the body. Studies have found that up to 85% to 90% of antibiotics enter the environment through excrement in the form of drug prototypes or metabolites, causing serious pollution to soil and water. Mo Cehui's research group mainly focused on the impact of antibiotic pollution on vegetable planting and agricultural food safety i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C02F3/34B09C1/10C02F103/34C12R1/645
CPCB09C1/105C02F3/347C02F2103/343C12N1/16C12N1/145C12R2001/645
Inventor 陈烁娜曾洁仪檀笑陈杨梅解启来韩伟江
Owner SOUTH CHINA AGRI UNIV
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