Selective synthesis method for 2, 5-dihydroxymethylfuran

A dimethylolfuran and selective technology, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve the difficulty of increasing product separation and purification, reduce economy and practicability, The catalytic system is complex and other problems, to achieve the effect of repeated use, easy separation and purification, and simple reaction process

Inactive Publication Date: 2018-10-02
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the catalytic system reported by He et al. is too complex, requiring the addition of a large number of co-substrates and other additives (200mM glucose, 400mM xylose, 250mM glutamic acid, 1.5mM Mg 2+ , 40mM β-cyclodextrin and 12.5mM cetyltrimethylammonium bromide), which not only greatly reduces the economy and practicability of the process, but also increases the difficulty of subsequent product separation and purification
At the same time, the reaction time of the biocatalytic process is too long, requiring 72h

Method used

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  • Selective synthesis method for 2, 5-dihydroxymethylfuran
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  • Selective synthesis method for 2, 5-dihydroxymethylfuran

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Pichia guilliermondii (Meyerozyma guilliermondii) SC1103 domestication and activation: Pichia guilliermondii SC1103 strains were inserted into yeast extract powder glucose (YPD) liquid medium (1% yeast extract, 2% peptone) containing 5mM HMF and 2% glucose), after culturing at 30°C and 200r / min for 12h, the above-mentioned bacterial suspension was inserted into fresh YPD liquid medium containing 5mM HMF with an inoculum size of 2%, and then incubated at 30°C and 200r / min Under culture for 12h. Subsequently, the above-mentioned bacterial suspension was successively introduced into YPD liquid medium containing 10 and 15 mM HMF with an inoculation amount of 2%, and similar steps were adopted to gradually acclimatize. Inoculate the above-mentioned acclimatized Pichia montagii SC1103 strain into the YPD liquid medium containing 15mM HMF, cultivate it at 30°C and 200r / min for 12h, and inoculate the above-mentioned bacterial suspension with an inoculum of 2%. Put into fresh Y...

Embodiment 2

[0029] Immobilization of Pichia mongolica SC1103: Weigh 2.5g of sodium alginate into a Erlenmeyer flask, add 100mL of distilled water, and heat to dissolve. After the solution was cooled to room temperature, the cells acclimated and activated in Example 1 were added at a concentration of 200 mg / mL-500 mg / mL (based on the wet weight of the cells). Add 0.2M CaCl drop by drop with a sterile syringe 2 After standing in the solution for 4 hours to solidify, the solution was washed with Tris-HCl buffer (100 mM, pH 7.2) for 3 times, and stored in a refrigerator at 4°C for use.

Embodiment 3

[0031] Add 50mM HMF and 30mM glucose to 4mL acetate buffer (50mM, pH 5.0), mix well, add the immobilized Jiyemeng prepared in Example 2 at a concentration of 20mg (wet cell weight) / mL (buffer solution) Pichia pastoris SC1103 cells were reacted at 35°C and 200r / min. The reaction was monitored by liquid chromatography ( image 3 ). After 9 h, liquid chromatography analysis showed that the yield of DHMF was 63%, and the selectivity was 99%.

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Abstract

The invention discloses a selective synthesis method for 2, 5-dihydroxymethylfuran. The method includes the steps of: (1) conducting domestication, activation and immobilization on meyerozyma guilliermondii SC1103 to obtain immobilized cells; (2) adding the immobilized cells into a buffer solution containing 50-350mM 5-hydroxymethylfurfural and cosubstrates, and carrying out reaction at 10-40DEG Cunder a stirring condition to obtain 2, 5-dihydroxymethylfuran. Specifically, the molar concentration of reducing sugar in the cosubstrates is 0.3-1.0 times that of 5-hydroxymethylfurfural. The immobilized biocatalyst provided by the invention has high tolerance to the substrate, can achieve efficient and high selective catalysis of high concentration substrate reduction to synthesize a target product, achieve a high product space time yield, and is beneficial to simplifying the subsequent separation and purification process of the target product.

Description

technical field [0001] The invention belongs to the field of biocatalysis and biomanufacturing, and in particular relates to a method for preparing 2,5-dimethylfuran by using immobilized yeast cells to catalyze the selective reduction of 5-hydroxymethylfurfural. Background technique [0002] In recent years, the development and utilization of bio-based energy and platform compounds have gradually attracted the attention of academia and industry. 5-hydroxymethylfurfural (5-hydroxymethylfurfural, HMF) is an important bio-based platform compound (Green Chem., 2010, 12, 539), which can be prepared by carbohydrate dehydration. HMF molecules have multiple active groups such as hydroxyl groups, aldehyde groups and carbon-carbon double bonds, so they are easily converted into various high value-added chemicals. 2,5-Dihydroxymethylfuran (2,5-dihydroxymethylfuran, DHMF) is a selective reduction product of aldehyde groups in HMF molecules, and has important application value in the fi...

Claims

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Application Information

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IPC IPC(8): C12P17/04C12N1/36C12N1/16C12N11/10C12R1/84
CPCC12N1/16C12N1/36C12N11/10C12P17/04
Inventor 李宁徐中华宗敏华程爱迪
Owner SOUTH CHINA UNIV OF TECH
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