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Auricularia auricula polysaccharide extracting method

A black fungus polysaccharide and extraction method technology, applied in the field of black fungus polysaccharide extraction, can solve the problems of high equipment requirements, long time consumption, easy inactivation, etc., achieve high economic benefits and avoid high equipment requirements

Inactive Publication Date: 2018-10-12
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems of the existing black fungus polysaccharide extraction method, such as low extraction rate, long time consumption, high price of enzyme, slow reaction conditions, easy inactivation, high equipment requirements, etc., the present invention provides a black fungus polysaccharide extraction method, using The technical scheme is as follows:

Method used

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  • Auricularia auricula polysaccharide extracting method

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Embodiment 1

[0045] The present embodiment provides a kind of extraction method of black fungus polysaccharide, and this method is carried out according to the following steps:

[0046] 1) drying the black fungus until the water content is 15%, to obtain the pretreated black fungus;

[0047] 2) Put the pretreated black fungus in step 1) into a pulverizer for freezing and crushing. The freezing and pulverizing is to first fill the pulverizer with liquid nitrogen, and then pulverize to obtain black fungus powder; wherein: the amount of liquid nitrogen charged 20% (v / m), the crushing condition is 30000rpm / min, 4min;

[0048] 3) Add water to the black fungus powder obtained in step 2) according to the solid-liquid ratio of 1:60, and perform ultrasonic extraction at 80°C and 300W for 15 minutes. After the extraction, centrifuge at 4500r / min for 6 minutes to take the supernatant Transfer the solution to a 100mL volumetric flask, repeat the above steps for the centrifuged precipitate, and mix th...

Embodiment 2

[0050] The present embodiment provides a kind of extraction method of black fungus polysaccharide, and this method is carried out according to the following steps:

[0051] 1) drying the black fungus until the water content is 1%, to obtain the pretreated black fungus;

[0052]2) Put the pretreated black fungus in step 1) into a pulverizer for freezing and crushing. The freezing and pulverization is to first fill the pulverizer with liquid nitrogen, and then pulverize to obtain black fungus powder; wherein: the filling amount of liquid nitrogen 5% (v / m), the crushing condition is 20000rpm / min, 1min;

[0053] 3) Add water to the black fungus powder obtained in step 2) according to the solid-liquid ratio of 1:20, and perform ultrasonic extraction at 40°C and 100W for 5 minutes. After the extraction, centrifuge at 4500r / min for 6 minutes to take the supernatant Transfer the solution to a 100mL volumetric flask, repeat the above steps for the centrifuged precipitate, and mix the ...

Embodiment 3

[0055] The present embodiment provides a kind of extraction method of black fungus polysaccharide, and this method is carried out according to the following steps:

[0056] 1) drying the black fungus to a water content of 10% to obtain pretreated black fungus;

[0057] 2) Put the pretreated black fungus in step 1) into a pulverizer for freezing and crushing. The freezing and pulverization is to first fill the pulverizer with liquid nitrogen, and then pulverize to obtain black fungus powder; wherein: the filling amount of liquid nitrogen 15% (v / m), the crushing condition is 25000rpm / min, 2min;

[0058] 3) Add water to the black fungus powder obtained in step 2) according to the solid-liquid ratio of 1:50, and perform ultrasonic extraction at 60°C and 180W for 9 minutes. After the extraction, centrifuge at 4500r / min for 6 minutes to take the supernatant Transfer the solution to a 100mL volumetric flask, repeat the above steps for the centrifuged precipitate, and mix the two sup...

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Abstract

The invention discloses an auricularia auricula polysaccharide extracting method, and belongs to the technical field of auricularia auricula deep processing. According to the method, auricularia auricula is dried until the water content reaches 1-15%, and the auricularia auricula is placed into a grinding machine for cryogenic grinding, wherein cryogenic grinding means that firstly, liquid nitrogen is introduced into the grinding machine, and then grinding is conducted to obtain auricularia auricula powder; water is added into the auricularia auricula powder for ultrasonic extraction, after extraction is completed, centrifugation is conducted to take supernate, the above steps are repeated for sediment after centrifugation, and an auricularia auricula polysaccharide extracting solution isobtained by mixing two-time supernate. According to the method, the cryogenic grinding mode is adopted for conducting cell wall breaking treatment on the auricularia auricula, meanwhile, auricularia auricula polysaccharide extraction is conducted supplemented by an ultrasonic technology, by implementing the method, the auricularia auricula grinding particle size can be improved to 125 mu m, the grinding rate is up to 74%, and the polysaccharide extraction efficiency is improved to 20%; by means of liquid nitrogen grinding treatment, dissolution of components with low molecular mass is promoted, the antioxidation effect is good, and accordingly the method is suitable for extracting polysaccharides in the auricularia auricula.

Description

technical field [0001] The invention relates to a method for extracting black fungus polysaccharides, belonging to the technical field of black fungus deep processing. Background technique [0002] Black fungus (Black fungus), also known as mulberry ear, tree chicken, wood velvet, etc., is a large fungus. Black fungus is sweet in taste, flat in nature, crisp and refreshing, delicious and nutritious. It can not only be cooked into delicious dishes, but also has high nutritional and medicinal value. It is edible and can be used as medicine. In the food industry, black fungus is used to develop into A variety of foods, such as black fungus health drinks, black fungus biscuits, black fungus sauce, black fungus spiced dried black fungus, black fungus instant powder, black fungus canned and black fungus bread, etc.; , nourishing the kidneys and lungs, nourishing qi and strengthening the body, lowering blood fat, anti-thrombosis and other functions, it is often used to make black ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06
CPCA61P39/06C08B37/0003C08B37/006
Inventor 韩雪白利琴李研东郝建雄王雅冰顾梦茹郭旭茜王雯赵丹丹袁雨菲张熙陈宜郭双凤魏鑫硕李嘉欣
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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