Method for extracting epsilon-PL (polylysine)

A technology of polylysine and polylysine bacteria, which is applied in the field of extraction and separation, can solve the problems of high production of ε-PL and wide application, and achieve the advantages of eliminating bacterial separation operation, simple process flow, and easy industrial scale-up Effect

Active Publication Date: 2018-10-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the high production of ε-PL has become the main factor limiting its wide application in the food industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Dilution of fermentation broth: Take 100 mL of ε-PL fermentation broth and add deionized water to dilute it by 2 times, then stir evenly;

[0058] (2) Resin adsorption ε-PL: Put 25g of activated hydrogen-form weak acid ion exchange resin Amberlite IRC-50 into the diluted fermentation broth, and measure the ε-PL content in the fermentation broth while stirring until it fails to detect To ε-PL;

[0059] (3) ε-PL desorption: After the adsorption is complete, stop stirring, after standing for 5 hours, remove the upper fermentation broth, add 500mL deionized water and stir to remove the residual fermentation broth and bacteria adsorbed on the resin surface, and finally use 500mL 0.2M HCl desorption for 4h under stirring conditions;

[0060] (4) ε-PL refining: adjust the pH value of the 500mL eluent to about 4.0, add 10g activated carbon, and raise the temperature to 80°C for 20 minutes; the decolorizing solution is desalted and concentrated by a 200Da nanofiltration membrane w...

Embodiment 2

[0063] (1) Dilution of fermentation broth: Take 100 mL of ε-PL fermentation broth and add deionized water to dilute it by 6 times, then stir evenly;

[0064] (2) Resin adsorption ε-PL: Put 20g of activated sodium-type weak acid ion exchange resin Amberlite IRC-50 into the diluted fermentation broth, and measure the ε-PL content in the fermentation broth while stirring, until the detection fails To ε-PL;

[0065] (3) ε-PL desorption: After the adsorption is completed, stop stirring, after standing for 4h, remove the resin, add it to the separation column, and use deionized water to remove the adsorbed on the column at a rate of 5BV / h or less. The bacteria and fermentation broth on the resin surface are finally eluted with 1M NaOH at a rate of 2BV / h.

[0066] (4) ε-PL refining: adjust the pH value of 400mL eluent to about 4.0, add 10g activated carbon, and heat to 85°C for 30 minutes; the decolorizing solution is desalted and concentrated by a 200Da nanofiltration membrane with a mole...

Embodiment 3

[0069] (1) Dilution of fermentation broth: Take 100 mL of ε-PL fermentation broth and add deionized water to dilute it by 5 times, then stir evenly;

[0070] (2) Resin adsorption ε-PL: Put 35g of activated ammonia-type weak acid ion exchange resin Amberlite IRC-50 into the diluted fermentation broth, and measure the ε-PL content in the fermentation broth while stirring until it is not detected. To ε-PL;

[0071] (3) ε-PL desorption: After the adsorption is completed, stop stirring, after standing for 6h, remove the resin, add it to the separation column, and use deionized water to remove the adsorbed on the column at a rate below 6BV / h. The bacteria and fermentation broth on the resin surface are finally eluted with 1M NH3·H2O at a rate of 2BV / h;

[0072] (4) ε-PL refining: adjust the pH value of the 600mL eluent to about 4.0, add 15g activated carbon, and heat to 85°C for 30 minutes; the decolorizing solution is desalted and concentrated by a 200Da nanofiltration membrane with a mo...

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Abstract

The invention discloses a method for extracting epsilon-PL (polylysine) and belongs to the technical field of extraction and separation. The method for directly separating and extracting epsilon-PL from unprocessed fermentation liquor by ion exchange resin is provided, and according to the method, extraction steps can be greatly reduced and the extraction cost is obviously reduced; with the adoption of the method for extracting epsilon-PL, the recovery rate can be as high as 75% or higher, the purity of a recovered finished product is as high as 98% or higher, and the recovery rate and the purity are increased by 15% and 2% respectively as compared with those achieved with the traditional ion exchange extraction method, so that the method has remarkable advantages.

Description

Technical field [0001] The invention relates to a method for extracting ε-polylysine, and belongs to the technical field of extraction and separation. Background technique [0002] ε-Polylysine (ε-PL) is a homologous amino acid polymer produced by extracellular secretion of microorganisms such as Streptomyces, filamentous fungi, or Bacillus. It is generally composed of 25-35 L-lysine monomers. α-COOH and ε-NH2 are dehydrated and condensed. The molecular weight is usually 2500-4500Da. [0003] Due to the advantages of good water solubility, strong thermal stability, and broad antibacterial spectrum, ε-PL is currently mainly used as a biological food preservative in the food industry in Japan, Korea, Europe and the United States and other countries and regions. In 2014, my country also officially approved the application of ε-PL and its hydrochloride in the food fields of fruits and vegetables, rice and noodle products, meat products, condiments, beverages and bakery. [0004] In fac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/10
Inventor 陈旭升张建华毛忠贵马玉张宏建王柯
Owner JIANGNAN UNIV
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