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A method for extracting ε-polylysine by resin in-situ adsorption

A polylysine and resin technology, applied in the field of extraction and separation, can solve the problems of high production and wide application of ε-PL, and achieve the effects of eliminating bacterial cell separation operation, low investment cost and simple process flow

Active Publication Date: 2019-09-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the high production of ε-PL has become the main factor limiting its wide application in the food industry.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Dilution of fermentation broth: take 100 mL of ε-PL fermentation broth and add deionized water to dilute it by 2 times, then stir evenly;

[0058] (2) Resin adsorption ε-PL: put 25g of activated hydrogen-type weak acid ion exchange resin Amberlite IRC-50 into the diluted fermentation broth, and detect the ε-PL content in the fermentation broth while stirring until no to ε-PL;

[0059] (3) Desorption of ε-PL: After the adsorption is completed, stop stirring, and after standing for 5 hours, remove the upper layer of fermentation broth, add 500mL deionized water and stir to remove the residual fermentation broth and bacteria adsorbed on the surface of the resin, and finally use 500mL 0.2M HCl was desorbed for 4h under stirring condition;

[0060] (4) Refinement of ε-PL: adjust the pH value of 500mL eluent to about 4.0, add 10g of activated carbon, raise the temperature to 80°C and keep for 20min; Get ε-PL finished product.

[0061] The recovery rate of ε-PL of this ...

Embodiment 2

[0063] (1) Dilution of fermentation broth: Take 100 mL of ε-PL fermentation broth and add deionized water to dilute it 6 times, then stir evenly;

[0064] (2) Resin adsorption ε-PL: put 20 g of activated sodium-type weak acid ion exchange resin Amberlite IRC-50 into the diluted fermentation broth, and detect the ε-PL content in the fermentation broth while stirring until no to ε-PL;

[0065] (3) Desorption of ε-PL: After the adsorption is completed, stop stirring, and after standing still for 4 hours, remove the resin, add it to the deionized column, and use deionized water to remove the adsorption at a rate of less than 5BV / h. Bacteria and fermentation broth on the surface of the resin, and finally ε-PL was eluted with 1M NaOH at a rate of 2BV / h;

[0066] (4) Refinement of ε-PL: adjust the pH value of 400mL eluent to about 4.0, add 10g of activated carbon, raise the temperature to 85°C and keep for 30min; Get ε-PL finished product.

[0067] The ε-PL yield of this example r...

Embodiment 3

[0069] (1) Dilution of fermentation broth: take 100 mL of ε-PL fermentation broth and add deionized water to dilute it 5 times, then stir evenly;

[0070] (2) Resin adsorption ε-PL: put 35g of activated ammonia-type weak acid ion exchange resin Amberlite IRC-50 into the diluted fermentation broth, and detect the ε-PL content in the fermentation broth while stirring until no to ε-PL;

[0071] (3) Desorption of ε-PL: After the adsorption is completed, stop stirring, and after standing still for 6 hours, remove the resin, add it to the deionized column, and use deionized water to remove the adsorption at a rate of less than 6BV / h. Bacteria and fermentation broth on the surface of the resin, and finally ε-PL was eluted with 1M NH3·H2O at a rate of 2BV / h;

[0072] (4) ε-PL refining: adjust the pH value of 600mL eluent to about 4.0, add 15g of activated carbon, raise the temperature to 85°C and keep for 30min; Get ε-PL finished product.

[0073] The ε-PL yield of this example rea...

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Abstract

The invention discloses a method for extracting epsilon-PL (polylysine) and belongs to the technical field of extraction and separation. The method for directly separating and extracting epsilon-PL from unprocessed fermentation liquor by ion exchange resin is provided, and according to the method, extraction steps can be greatly reduced and the extraction cost is obviously reduced; with the adoption of the method for extracting epsilon-PL, the recovery rate can be as high as 75% or higher, the purity of a recovered finished product is as high as 98% or higher, and the recovery rate and the purity are increased by 15% and 2% respectively as compared with those achieved with the traditional ion exchange extraction method, so that the method has remarkable advantages.

Description

technical field [0001] The invention relates to a method for extracting ε-polylysine by resin in-situ adsorption, and belongs to the technical field of extraction and separation. Background technique [0002] ε-polylysine (ε-PL) is a homotype amino acid polymer produced by extracellular secretion of microorganisms such as Streptomyces, filamentous fungi or Bacillus, which generally consists of 25-35 L-lysine monomers through It is formed by dehydration condensation of α-COOH and ε-NH2, and its molecular weight is usually 2500-4500Da. [0003] Due to the advantages of good water solubility, strong thermal stability, and broad antibacterial spectrum, ε-PL is currently mainly used as a biological food preservative and widely used in the food industry in Japan, South Korea, Europe, America and other countries and regions. In 2014, my country also officially approved the application of ε-PL and its hydrochloride in the food fields such as fruits and vegetables, rice and flour pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G69/10
Inventor 陈旭升张建华毛忠贵马玉张宏建王柯
Owner JIANGNAN UNIV
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