Three-dimensional coiled structure with function of automatically adjusting structure, as well as preparation method and applications of three-dimensional coiled structure

A technology for adjusting the structure and three-dimensional tubular shape, applied in the fields of medical materials and tissue engineering, can solve the problems of organ transplantation failure, abnormal antibacterial problem, patient death, etc., and achieve the effect of simple preparation

Active Publication Date: 2018-10-16
柔脉医疗(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the failure of tissue or organ transplantation caused by acute or chronic bacterial infection, or even the death of the patient directly, is very serious in clinical practice. protrude

Method used

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  • Three-dimensional coiled structure with function of automatically adjusting structure, as well as preparation method and applications of three-dimensional coiled structure
  • Three-dimensional coiled structure with function of automatically adjusting structure, as well as preparation method and applications of three-dimensional coiled structure
  • Three-dimensional coiled structure with function of automatically adjusting structure, as well as preparation method and applications of three-dimensional coiled structure

Examples

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Comparison scheme
Effect test

Embodiment 1

[0080] This example is used to illustrate the preparation method of electrospun film, and the specific scheme and results are shown in Figure 3a-c and Figure 4 a-e.

[0081] First, two degradable polymer materials with different degradation rates, PLGA and PCL, were selected as the basic materials of the scaffold, and hexafluoroisopropanol, dichloromethane, acetone, N,N-dimethylformamide (DMF), etc. As a solvent, stir and mix well for the preparation of electrospinning films.

[0082] Using electrospinning technology, apply a high-voltage power supply of 15kV, select hexafluoroisopropanol as a solvent, and prepare two kinds of polymer solutions, wherein the concentration of PCL solution is 10wt%, the concentration of PLGA solution is 20wt%, and the receiving distance is 15cm. The final PLGA The diameter is about 0.7 μm, the diameter of PCL is about 0.6 μm, and the porosity is 70%. Finally, an electrospun film with a thickness of about 80 μm is prepared. The PLGA layer with ...

Embodiment 2

[0084] This example is used to illustrate the surface modification of electrospun film and the two-dimensional patterning of cells. For the specific scheme and results, see image 3 d-f, Figure 4 f and Figure 5 a.

[0085]First, design and process a PMMA template with a three-channel (or multi-channel) structure, pour it with a PDMS solution (mass ratio of matrix:curing agent 10:1), place it in an oven at 80°C for 1 hour, and remove the cured PDMS from the template Take it off, cut the edge, and use a puncher to punch three channels (or multi-channels) (aperture 8mm), and prepare microfluidic planting chips in turn, and use the prepared three-channel cells to plant microfluidic chips. For the regionalized planting of cells, after attaching the PDMS chip to the surface of the electrospun film, first add Fibronectin (50 μg / ml) to modify the spinning surface to facilitate the adhesion of cells; In the channel, add endothelial cells (containing VEGF), smooth muscle cells, fib...

Embodiment 3

[0087] This example is used to illustrate the three-dimensional transformation of the two-dimensional patterned film, and the specific scheme and results are shown in Figure 3i-h, Figure 4 g-i and Figure 5 b-c.

[0088] Predesigned tension-induced self-rolling films ( image 3 c), it has a double-layer structure, the bottom layer is an electrospun drug-loaded film, and the upper layer is a pre-stretched PDMS strip with a strain of 70% and a thickness of about 80 μm. The spun films are pasted together to form a complete layer. Since PDMS has a certain internal stress after pre-stretching, when the cells are attached, use a scalpel to cut the film and PDMS strip from the right side, and the film will automatically curl to form a multi-layer roll structure with a specific inner diameter. . The structure has 6 layers, which are: endothelial layer, PCL layer, smooth muscle cell layer, PLGA layer, fibroblast / nerve cell layer, PLGA layer from inside to outside.

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Abstract

The invention provides a three-dimensional coiled structure with the function of automatically adjusting the structure, as well as a preparation method and applications of the three-dimensional coiledstructure. The three-dimensional multi-layer coiled structure has the function of automatically adjusting the self structure aiming at the external environment, and can be taken as the tissue engineering blood vessel similar to the real vascular structure to be applied to the fields including medical materials, tissue engineering and the like.

Description

technical field [0001] The invention belongs to the fields of medical materials and tissue engineering. Specifically, the present invention relates to a three-dimensional roll-like structure with the function of self-adjusting structure, its preparation method and application. Background technique [0002] According to the statistics of the World Health Organization, heart disease, especially coronary artery disease, has always been the number one cause of death that threatens human life. Among them, coronary atherosclerotic heart disease (coronary heart disease) is the disease with the highest fatality rate among acquired diseases. It is mainly due to the accumulation of sterols and other substances on the inner wall of blood vessels to form different degrees of plaque, which will greatly reduce the need for blood flow. The section of the vascular lumen will cause insufficient blood supply to the downstream tissues and damage the body. Coronary heart disease generally cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/38A61L27/18A61L27/24A61L27/50A61L27/58B32B1/08B32B27/36B32B27/28B32B3/08
CPCA61L27/18A61L27/24A61L27/3891A61L27/50A61L27/507A61L27/58B32B1/08B32B3/08B32B27/28B32B27/283B32B27/36B32B2535/00
Inventor 蒋兴宇成诗宇
Owner 柔脉医疗(深圳)有限公司
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