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Immumofluorescence method kit used for detecting placental growth factor

A placental growth factor and immunofluorescence technology, which is applied in the field of immunofluorescence kits for placental growth factor detection, can solve the problems of the need for replacement of electrodes, insufficient sensitivity, high background signal, etc., so as to eliminate the interference of non-specific fluorescence and improve the High analytical sensitivity and high fluorescence intensity

Pending Publication Date: 2018-10-19
NINGBO AUCHEER BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the enzyme-linked immunoassay is cheap and fast, it is not sensitive enough and is only suitable for the detection and identification of trace substances; electrochemiluminescence requires the use of three electrodes to carry out an unknown complex reaction between multiple electroactive samples. The electrode needs to be replaced and is expensive, the electrochemiluminescence method has a high background signal due to the electrochemiluminescence caused by the high concentration of TPA, etc.

Method used

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  • Immumofluorescence method kit used for detecting placental growth factor
  • Immumofluorescence method kit used for detecting placental growth factor
  • Immumofluorescence method kit used for detecting placental growth factor

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0030] Such as Figure 1-8As shown, an immunofluorescence kit for detection of placental growth factor comprises a kit box body 1 and a test strip 2 arranged in the kit box body, the kit box body includes a housing 3, a protective cover 4, The housing 3 and the protective sleeve 4 are connected by clamping. The housing 3 includes an upper housing 5 and a lower housing 6. The upper housing 5 includes an upper housing upper part 7, an upper housing middle part 8, and an upper housing whose width increases successively. The lower part 9, the upper part of the upper casing is provided with a sample injection hole 10, the sample injection hole 10 is a circular structure, the lower part of the upper casing 9 is provided with a detection window 11, and the detection window 11 is a rectangular structure, and the inner surface of the upper casing 5 is provided with There are a first card body 12, a first card strip 13, and a second card strip 14, the first card body 12 is a cylindrical...

Embodiment 2

[0047] This embodiment is basically the same as Embodiment 1, the difference is:

[0048] A preparation method for immunofluorescence test strips for placental growth factor detection, comprising the steps of:

[0049] (1) Preparation of placental growth factor monoclonal antibody conjugates labeled with europium fluorescent nanospheres: dissolve europium fluorescent nanospheres in CBS buffer (50 mM, pH=9.6), wash 3 times by centrifugation, and the centrifugation speed is 18000rpm for 3 minutes, finally resuspend in CBS buffer (50mM, pH=9.6), add aldylated dextran, mix well, react in dark at room temperature for 5 hours, after the reaction is completed, use the above centrifugation method Wash and then resuspend in CBS buffer (50mM, pH=9.6) to obtain formylated europium fluorescent nanospheres, which are stored at 4°C for later use; placenta growth factor monoclonal antibody conjugate labeled with europium fluorescent nanospheres Preparation of placental growth factor monoclo...

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Abstract

The invention discloses an immumofluorescence method kit used for detecting placental growth factor. The immumofluorescence method kit used for detecting placental growth factor comprises a kit box body, and a test paper strip; the test paper strip is composed of a substrate, a water absorbing pad, a coated analysis membrane, a coupling product pad, and a sample pad; the coated analysis membrane is provided with a detection line, and a quality control line; the antibody used for coating the detection line is anti-placental growth factor monoclonal antibody; the antibody used for coating the quality control line is rabbit IgG antibody; the coupling product pad is coated with an europium fluorescence nanometer sphere labeled placental growth factor monoclonal antibody coupling product. The immumofluorescence method kit is provided with a protective cover, so that sampling hole pollution caused byimproper operation is avoided; an upper housing and a lower housing are adopted, so that testpaper strip can be pressed, test paper strip shedding and deviating are avoided, and test result reliability is ensured. According to the test paper strip, time resolved immunofluorometric assay technology is adopted in detection of placental growth factor, so that the analysis sensitivity is increased greatly, and active meaning on placental growth factor quantitative determination is achieved.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to an immunofluorescence assay kit for detecting placental growth factor. Background technique [0002] Placental growth factor (PLGF) was first isolated and purified from human placental cDNA library by Maglione et al. in 1991. PLGF is mainly synthesized by syncytiotrophoblast cells and can bind to tyrosinase receptors located in trophoblast cells and vascular endothelial cells. It is a protein that has autocrine effects on trophoblast cell functions and paracrine effects on angiogenesis. PLGF has a unique regulatory effect on the function of trophoblast cells and endothelial cells, and can promote neovascularization. Detecting the blood PLGF level of pregnant women can be used clinically to identify the oxygen supply pressure of placental syncytiotrophoblast cells, and to predict, identify and monitor treatment of preeclampsia. [0003] So far, the methods used to detect placen...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/533G01N21/64
CPCG01N21/6408G01N21/6428G01N21/6486G01N33/533G01N33/577
Inventor 唐静陈星星蒋理国周义正
Owner NINGBO AUCHEER BIOTECHNOLOGY CO LTD
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