Immumofluorescence method kit used for detecting placental growth factor
A placental growth factor and immunofluorescence technology, which is applied in the field of immunofluorescence kits for placental growth factor detection, can solve the problems of the need for replacement of electrodes, insufficient sensitivity, high background signal, etc., so as to eliminate the interference of non-specific fluorescence and improve the High analytical sensitivity and high fluorescence intensity
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[0029] Example 1
[0030] Such as Figure 1-8 As shown, an immunofluorescence kit for detecting placental growth factor includes a kit body 1 and a test strip 2 arranged in the kit body, the kit body includes a housing 3 and a protective cover 4, The housing 3 and the protective sleeve 4 are connected by clamping. The housing 3 includes an upper housing 5 and a lower housing 6. The upper housing 5 includes an upper housing upper part 7, a middle upper housing 8 and an upper housing with increasing widths. The lower part 9, the upper part of the upper shell is provided with a sample hole 10, the sample hole 10 has a circular structure, the lower part 9 of the upper shell is provided with a detection window 11, the detection window 11 is a rectangular structure, and the inner surface of the upper shell 5 is provided There are a first card body 12, a first card bar 13, and a second card bar 14. The first card body 12 has a cylindrical structure, and the first card bar 13 and the se...
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[0046] Example 2
[0047] This embodiment is basically the same as embodiment 1, but the differences are:
[0048] A preparation method of immunofluorescence test strips for placental growth factor detection includes the following steps:
[0049] (1) Preparation of placental growth factor monoclonal antibody conjugate labeled with Europium fluorescent nanospheres: Dissolve Europium fluorescent nanospheres in CBS buffer (50mM, pH=9.6), and wash 3 times by centrifugation at a speed of 18000rpm, time is 3 minutes, finally resuspend in CBS buffer (50mM, pH=9.6), add aldehyde-based dextran, mix, and react in the dark at room temperature for 5 hours. After the reaction is completed, use the above-mentioned centrifugal method Washed, then resuspended in CBS buffer (50mM, pH=9.6) to obtain aldehyde-based europium fluorescent nanospheres, placed at 4°C for use; Europium fluorescent nanospheres labeled placental growth factor monoclonal antibody conjugate Preparation: Placental growth factor...
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