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Method for extracting and separating proanthocyanidins from lycium ruthenicum murr

A technology of black fruit wolfberry and proanthocyanidins, applied in the field of health food and medicine, plant active ingredient extraction, and food, can solve the problems of not meeting quality requirements, low content of oligomers and polyphenols, etc., and achieve low cost and high osmotic pressure , the effect of high product quality

Inactive Publication Date: 2018-10-23
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, the current research on proanthocyanidins in my country is mainly based on solvent extraction. It is difficult to further separate and purify the mixture of proanthocyanidins, so that the content of oligomers and polyphenols in proanthocyanidins products is low, which cannot meet the quality requirements of some European and American suppliers. , so the product can only be sold as a crude product at a low price

Method used

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  • Method for extracting and separating proanthocyanidins from lycium ruthenicum murr

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Take black wolfberry, crush it, and pass through a 40-mesh sieve; add 1.0 kg of black wolfberry powder into a multifunctional extraction tank, add 12L of 60% ethanol solution, add 10 g of citric acid and 15 g of malic acid, and turn on the high-speed shear Cut off the machine, set the speed at 10000rpm; start stirring, set the speed at 120rpm; start timing when the temperature rises to 60°C, and extract for 0.5 h; filter the extract, then add 10L of 60% ethanol to extract under the same conditions, and combine 2 secondary extract;

[0028] 2) Concentrate the above extract under reduced pressure and vacuum until there is no alcohol smell: the temperature is 50°C, and the pressure is 0.07MPa;

[0029] 3) Pump the concentrated extract obtained in step 2) into the pre-treated macroporous adsorption resin mixed resin simulation bed (two resin columns connected in series, LSA-10: XDA-6: D101=2: 3:5 , the ratio of diameter to height is 1:12), the sample loading flow rate i...

Embodiment 2

[0033]1) Take 25 kg of black wolfberry powder that has been crushed through a 40-mesh sieve, put it in a multi-functional extraction tank, add 15 times the amount of 70% ethanol, turn on the high-speed shearing machine, set the speed at 10,000 RPM, and start stirring at the same time, Set the speed at 120RPM. When the temperature rises to 60°C, start timing. Extract for 1 h, let it stand for 2 h, and drain the extract from the drain port on the side of the extraction tank;

[0034] 2) Concentrate the above extract with a double-effect concentrator to recover alcohol until there is no alcohol smell, at a temperature of 50°C and a pressure of 0.075MPa;

[0035] 3) Pump the concentrated extract obtained in step 2) into the pre-treated macroporous adsorption resin mixed resin simulation bed (two resin columns connected in series, LSA-10: XDA-6: D101=2: 3:5 , the ratio of diameter to height is 1:12), the sample loading flow rate is 1.5 BV / HR, after the sample is loaded, it is fir...

Embodiment 3

[0039] 1) Take 1 kg of black wolfberry powder crushed through a 40-mesh sieve, put it in a multi-functional extraction tank, add 12 times the amount of 60% ethanol for the first time, and add 10 times the amount of 60% ethanol for the second time. Turn on the high-speed shearing machine, set the rotating speed to be 10000 RPM, and simultaneously turn on the stirring, and set the rotating speed to be 120 RPM. When the temperature rises to 60°C, start timing. Extract for 0.5 h, extract twice; stand for 2 h, drain the extract from the drain port on the side of the extraction tank, and combine the extract;

[0040] 2) Use a vacuum rotary evaporator to recover alcohol from the above extract until there is no alcohol smell, at a temperature of 50°C and a pressure of 0.075MPa;

[0041] 3) Pump the concentrated extract obtained in step 2) into the pre-treated macroporous adsorption resin mixed resin simulation bed (two resin columns connected in series, LSA-10: XDA-6: D101=2: 3:5 , ...

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Abstract

The invention discloses a method for extracting and separating proanthocyanidins from lycium ruthenicum murr. According to the method, a high-speed shearing wall-breaking technology, a macroporous resin mixed simulated moving bed enrichment technology and a nanofiltration membrane separation and purification technology are adopted as core technologies, lycium ruthenicum murr with the highest content of proanthocyanidins is used as a raw material, and green solvents water and ethanol are used as solvents to develop a proanthocyanidins industrialization technology and its products in line with the international mainstream product specifications. The content of proanthocyanidins in the obtained product is greater than or equal to 85%, OPC: 65%-80%, and polyphenol content is greater than or equal to 85%. The process not only helps improve the purity and yield of oligoprocyanidins, but also meets the requirements of industrial production on raw materials, solvent use, production route, production process safety, product color, yield and purity. The process is a pure green production process. by the process, there is organic residues, output is high, and product quality can reach the standards of mainstream products in the international market. The product can be used as a raw material for food, health food and special medical uses.

Description

technical field [0001] The invention relates to a method for separating and extracting proanthocyanidins from Lycium barbarum, in particular to a method for separating and extracting proanthocyanidins from Lycium barbarum with a mixed simulated bed of macroporous adsorption resin, belonging to the technical field of extracting plant effective components, and is mainly used for Food, health food and pharmaceutical technology fields. Background technique [0002] Proanthocyanidin (PC) is a general term for a large class of polyphenolic compounds that widely exist in the plant kingdom. These compounds are polymerized by different numbers of flavanols. According to the degree of polymerization, two to tetramers are usually called oligomers (OPC for short), and those above pentamers are called high polymers (PPC for short). Among all kinds of proanthocyanidins, the antioxidant activity of polymers is lower than that of monomers, dimers and trimers, and oligomeric proanthocyanidi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07G99/00C07D311/54
CPCC07D311/54C07G99/00
Inventor 邸多隆刘建飞裴栋
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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