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Compound microbial preparation for soil remediation and preparation method and application of compound microbial preparation for soil remediation

A technology for compound microorganisms and soil remediation, applied in the field of compound microorganism preparations and their preparation, can solve the problems of low ammonia nitrogen removal rate, unsuitable for large-area application, easy inactivation and the like

Active Publication Date: 2018-10-26
江苏世邦生物工程科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The remediation technology and method of soil pollution have been studied abroad since the late 1970s and early 1980s. In the early stage, physical and chemical methods were generally used to remediate contaminated soil, such as heat treatment and chemical leaching, but they were expensive and not suitable for large areas. application
In the late 1980s and early 1990s, foreign countries began to study bioremediation methods based on the catabolism of microorganisms and plants based on physical and chemical processes. In recent years, they have developed very rapidly. They are not only more economical than physical and chemical methods, but also less prone to secondary pollution. pollution, it is more suitable for large-scale soil remediation, and bioremediation technology has begun to be the main treatment technology for soil remediation, and has played an increasingly important role. Therefore, it is urgent to comprehensively solve and repair the problems of heavy metal pollution, ammonia nitrogen pollution and organic matter pollution in the soil.

Method used

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  • Compound microbial preparation for soil remediation and preparation method and application of compound microbial preparation for soil remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 is used for the preparation of the composite microbial preparation of soil remediation

[0022] The preparation method of discoid spiral cyanobacteria (Spirulina platensis) bacterial agent comprises: discoid spiral cyanobacterium (Spirulina platensis) is cultivated to logarithmic phase, then will be cultivated to the bacterium of logarithmic phase by the inoculation amount of medium volume 3%. Seed is inoculated in the culture medium, shakes 220rpm at 30 ℃, cultivates for 48 hours, obtains the fermented liquid of Spirulina platensis (Spirulina platensis), adds gac to this fermented liquid, and dries to obtain Spirulina platensis ( Spirulina platensis) bacterial agent. The medium is 1.5g / L sodium acetate, 0.06g / L peptone, 0.8g / L sodium bicarbonate, 0.5g / L sodium thiosulfate, 0.3g / L sodium chloride, 0.2g / L magnesium sulfate and 0.07 g / L potassium dihydrogen phosphate. The live bacteria content of this plate-shaped spiral cyanobacteria (Spirulina platensis) ...

Embodiment 2

[0026] Embodiment 2 is used for the preparation of the composite microbial preparation of soil remediation

[0027] The preparation method of discoid spiral cyanobacteria (Spirulina platensis) bacterial agent comprises: discoid spiral cyanobacterium (Spirulina platensis) is cultivated to logarithmic phase, then will be cultured to the bacterium of logarithmic phase by the inoculation amount of 10% of culture medium volume Seeds are inoculated in the culture medium, 35 DEG C, shaking table shakes 180rpm, cultivates for 40 hours, obtains the fermented liquid of Spirulina platensis (Spirulina platensis), adds adsorbent to this fermented liquid, dries, promptly obtains Spirulina platensis Bacteria (Spirulina platensis) bacterial agent; the adsorbent is one or more of bentonite or activated carbon. The medium is 1.5g / L sodium acetate, 0.06g / L peptone, 0.8g / L sodium bicarbonate, 0.5g / L sodium thiosulfate, 0.3g / L sodium chloride, 0.2g / L magnesium sulfate and 0.07g / L Potassium dihyd...

Embodiment 3

[0031] Embodiment 3 is used for the preparation of the compound microbial preparation of soil remediation

[0032] The preparation method of discoid spiral cyanobacteria (Spirulina platensis) bacterial agent comprises: discoid spiral cyanobacterium (Spirulina platensis) is cultivated to logarithmic phase, then will be cultured to the bacterium of logarithmic phase by the inoculation amount of medium volume 5%. Seeds are inoculated into the culture medium, 33 DEG C, shaking table shakes 200rpm, cultivates for 44 hours, obtains the fermented liquid of Spirulina platensis (Spirulina platensis), adds bentonite to this fermented liquid, dries, promptly obtains Spirulina platensis (Spirulina platensis) bacterial agent. The medium is 1.5g / L sodium acetate, 0.06g / L peptone, 0.8g / L sodium bicarbonate, 0.5g / L sodium thiosulfate, 0.3g / L sodium chloride, 0.2g / L magnesium sulfate and 0.07g / L Potassium dihydrogen phosphate. The live bacteria content of described disc spiral cyanobacteria...

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Abstract

The invention discloses a compound microbial preparation for soil remediation. The compound microbial preparation comprises a spirulina platensis agent, a corynebacterium glutamicum ATCC13032 agent and a Hyphomicrobium denitrificans CGMCC No.10620 agent. The invention further discloses a preparation method and application of the compound microbial preparation for soil remediation. By joint use ofthe three microbial agents, effects of nitrogen and phosphorus removal and heavy metal adsorption are much superior to those of single and pairwise joint use of the bacterial agents, the ammonium-nitrogen removal rate reaches 95% or above, the P removal rate reaches 95%, and the heavy metal Cu and Cd removal rate reaches 96% or above.

Description

[0001] Technical solutions [0002] The invention belongs to the technical field of soil remediation, and in particular relates to a composite microbial preparation for remediation of soil, a preparation method and application thereof. Background technique [0003] Soil is the target of pollutants in the atmosphere, water and solid waste that migrate, flow and deposit in the environment. It is the bearer of long-term environmental pollution. With the rapid development of social economy and industrialization, some industrial wastewater has caused soil heavy metal pollution. Ammonia nitrogen pollution, organic pollution, etc. [0004] The remediation technology and method of soil pollution have been studied abroad since the late 1970s and early 1980s. In the early stage, physical and chemical methods were generally used to remediate contaminated soil, such as heat treatment and chemical leaching, but they were expensive and not suitable for large areas. application. In the lat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/15C12R1/01
CPCC12N1/20B09C1/10
Inventor 谢悦波陈求稳何庆成金波姚竣耀
Owner 江苏世邦生物工程科技有限公司