Compound microbial preparation for soil remediation and preparation method and application of compound microbial preparation for soil remediation
A technology for compound microorganisms and soil remediation, applied in the field of compound microorganism preparations and their preparation, can solve the problems of low ammonia nitrogen removal rate, unsuitable for large-area application, easy inactivation and the like
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Embodiment 1
[0021] Embodiment 1 is used for the preparation of the composite microbial preparation of soil remediation
[0022] The preparation method of discoid spiral cyanobacteria (Spirulina platensis) bacterial agent comprises: discoid spiral cyanobacterium (Spirulina platensis) is cultivated to logarithmic phase, then will be cultivated to the bacterium of logarithmic phase by the inoculation amount of medium volume 3%. Seed is inoculated in the culture medium, shakes 220rpm at 30 ℃, cultivates for 48 hours, obtains the fermented liquid of Spirulina platensis (Spirulina platensis), adds gac to this fermented liquid, and dries to obtain Spirulina platensis ( Spirulina platensis) bacterial agent. The medium is 1.5g / L sodium acetate, 0.06g / L peptone, 0.8g / L sodium bicarbonate, 0.5g / L sodium thiosulfate, 0.3g / L sodium chloride, 0.2g / L magnesium sulfate and 0.07 g / L potassium dihydrogen phosphate. The live bacteria content of this plate-shaped spiral cyanobacteria (Spirulina platensis) ...
Embodiment 2
[0026] Embodiment 2 is used for the preparation of the composite microbial preparation of soil remediation
[0027] The preparation method of discoid spiral cyanobacteria (Spirulina platensis) bacterial agent comprises: discoid spiral cyanobacterium (Spirulina platensis) is cultivated to logarithmic phase, then will be cultured to the bacterium of logarithmic phase by the inoculation amount of 10% of culture medium volume Seeds are inoculated in the culture medium, 35 DEG C, shaking table shakes 180rpm, cultivates for 40 hours, obtains the fermented liquid of Spirulina platensis (Spirulina platensis), adds adsorbent to this fermented liquid, dries, promptly obtains Spirulina platensis Bacteria (Spirulina platensis) bacterial agent; the adsorbent is one or more of bentonite or activated carbon. The medium is 1.5g / L sodium acetate, 0.06g / L peptone, 0.8g / L sodium bicarbonate, 0.5g / L sodium thiosulfate, 0.3g / L sodium chloride, 0.2g / L magnesium sulfate and 0.07g / L Potassium dihyd...
Embodiment 3
[0031] Embodiment 3 is used for the preparation of the compound microbial preparation of soil remediation
[0032] The preparation method of discoid spiral cyanobacteria (Spirulina platensis) bacterial agent comprises: discoid spiral cyanobacterium (Spirulina platensis) is cultivated to logarithmic phase, then will be cultured to the bacterium of logarithmic phase by the inoculation amount of medium volume 5%. Seeds are inoculated into the culture medium, 33 DEG C, shaking table shakes 200rpm, cultivates for 44 hours, obtains the fermented liquid of Spirulina platensis (Spirulina platensis), adds bentonite to this fermented liquid, dries, promptly obtains Spirulina platensis (Spirulina platensis) bacterial agent. The medium is 1.5g / L sodium acetate, 0.06g / L peptone, 0.8g / L sodium bicarbonate, 0.5g / L sodium thiosulfate, 0.3g / L sodium chloride, 0.2g / L magnesium sulfate and 0.07g / L Potassium dihydrogen phosphate. The live bacteria content of described disc spiral cyanobacteria...
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