Preparation method, product and application of a pH-responsive nano-silver assembly

A nano-silver and assembly technology, which is applied in the field of preparation of pH-responsive nano-silver assemblies, can solve the problems of increasing the dosage of drugs, difficult to kill biofilm bacteria and drug-resistant bacteria, and achieves inhibiting and destroying bacterial biofilms. , good clinical transformation possibility, the effect of accelerating surface corrosion

Active Publication Date: 2020-08-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it is difficult for a single antibacterial drug to effectively kill biofilm bacteria and the resulting drug-resistant bacteria
At present, the combination of antibacterial drugs is used to inhibit and destroy the biofilm structure in clinical practice, but it is often necessary to increase the dosage of drugs to achieve the goal, and there is a safety hazard of promoting bacterial resistance

Method used

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  • Preparation method, product and application of a pH-responsive nano-silver assembly
  • Preparation method, product and application of a pH-responsive nano-silver assembly
  • Preparation method, product and application of a pH-responsive nano-silver assembly

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1: Preparation of silver nanoparticles

[0034] Mix 170 mg of silver nitrate with 0.5 mL of oleylamine and 4.5 mL of oleic acid, stir at 70 °C for 1.5 hours under vacuum, heat the mixture to 180 °C at a rate of 1 °C / min, and keep the temperature when the system temperature reaches 180 °C temperature for 2 minutes, rapid cooling, adding a mixed solution of methanol and ethanol for precipitation, and centrifuging to obtain silver nanoparticles. The morphology of silver nanoparticles was characterized by transmission electron microscopy, and the results were as follows: figure 1 As shown, the particle size is about 3-5 nm.

Embodiment 2

[0035] Embodiment 2: Preparation of silver nanoparticles

[0036] Mix 170 mg of silver nitrate with 1 mL of oleylamine and 5 mL of oleic acid, stir at 70 °C for 2 hours under vacuum, heat the mixture to 180 °C at a speed of 10 °C / min, and cool it rapidly when the system temperature reaches 180 °C. A mixed solution of methanol and ethanol is added for precipitation, and centrifuged to obtain silver nanoparticles, and the size of the obtained silver nanoparticles is 6-8nm.

Embodiment 3

[0037] Embodiment 3: Preparation of polyethylene glycol-poly(aspartic acid-imidazole)-polyalanine

[0038] Dissolve 0.6g of polyethylene glycol-polyaspartate-polyalanine in 5mL of N,N-dimethylformamide, add 1mL of 1-(3-aminopropyl)imidazole, and Reaction at room temperature for 12h. Subsequently, the reaction solution was added to 25 mL of 0.1N dilute hydrochloric acid, and transferred to a dialysis bag (molecular weight cut-off: 3500), and dialyzed with 0.01N dilute hydrochloric acid as the dialysis medium. Finally, freeze-dry to obtain polyethylene glycol-poly(aspartic acid-imidazole)-polyalanine solid.

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Abstract

The invention relates to a preparation method of a pH-responsive nanosilver assembly. The preparation method comprises the following steps: 1), dissolving silver nitrate in oleylamine and oleic acid,heating for a reduction reaction, and precipitating by using a poor solvent to obtain silver nanoparticles; 2), dissolving an amphiphilic polymer in a good solvent, adding a modifier having a pH-responsive group, stirring at room temperature for a reaction, and dialyzing to obtain a pH-responsive amphiphilic polymer; 3), by a film dispersion method or an emulsion solvent evaporation method, assembling the silver nanoparticles obtained in the step 1) into the pH-responsive amphiphilic polymer obtained in the step 1) to obtain the pH-responsive nanosilver assembly. The invention further relatesto the pH-responsive nanosilver assembly prepared by the preparation method and application thereof. The pH-responsive nanosilver assembly has good antibacterial performance, not only can effectivelyinhibit growth of medicine-resistant bacteria, but also has the effects of inhibiting and destroying a bacterial biofilm.

Description

technical field [0001] The invention relates to the field of preparation of nano-silver composite materials, in particular to a preparation method, product and application of a pH-responsive nano-silver assembly. Background technique [0002] Antimicrobials play an important role in ensuring public health security in our country. However, due to the irrational use of antibacterial drugs, the situation of bacterial resistance in my country is severe. [0003] Bacteria often exist in the form of biofilm in biological tissues, which is a form corresponding to planktonic bacteria formed during the growth process of bacteria to adapt to the living environment, and is considered to be one of the important factors causing bacterial drug resistance. Bacterial biofilms have complex structures and diverse components, and participate in the formation of bacterial drug resistance through a variety of mechanisms, including osmotic limitation theory, nutrient limitation theory, expressio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K33/38A61P31/04
CPCA61K9/0002A61K9/5146A61K33/38
Inventor 凌代舜吴佳禾李方园高建青
Owner ZHEJIANG UNIV
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