A kind of construction method of mouse lung cancer model

A construction method, mouse technology, applied in the direction of pharmaceutical formula, medical preparations containing active ingredients, drug delivery, etc., can solve the problems of cumbersome operation, death of experimental animals, time-consuming operation, etc. Simple, wide-ranging effects

Active Publication Date: 2019-11-26
SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the nasal instillation method uses a pipette gun to suck the infusion solution and drip it into the nasal cavity of the animal drop by drop. Injection into the lungs may cause pneumothorax, hemothorax, infection, etc. to cause death of experimental animals due to multiple punctures, and the operation is cumbersome. The incidence of tumors in the gastrointestinal tract is relatively high, the specificity of carcinogenic organs is not ideal, and there is a large difference from the actual exposure of benzopyrene to lung cancer
Therefore, the above-mentioned construction methods for inducing lung cancer models have various deficiencies, and it is difficult to meet the relevant requirements.

Method used

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  • A kind of construction method of mouse lung cancer model
  • A kind of construction method of mouse lung cancer model
  • A kind of construction method of mouse lung cancer model

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Experimental program
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Effect test

Embodiment 1

[0034] The processing of embodiment one mouse

[0035] SPF grade C57BL / 6J wild-type mice were taken, and after one week of adaptation, they were randomly divided into two groups, 48 ​​in the control group and 48 in the exposure group, with half male and half male. The mice in the exposure group were exposed in the main cabinet of the dynamic inhalation exposure cabinet for 25 weeks, 5 days / week, 6h / day, and the specific exposure condition parameters are shown in Table 1.

[0036] Table 1 Parameters of dynamic inhalation exposure conditions

[0037]

[0038] During the exposure period, the mice were allowed to drink water freely, and the food supply was stopped. After the exposure, the mice were moved out of the mobile inhalation exposure cabinet, and the mice were allowed to drink water and eat freely. Throughout the process, observe the living conditions and body weight changes of the experimental animals, record the experimental animal body weight once a week, and the re...

Embodiment 2

[0039] Embodiment two BaP content detection

[0040] In order to ensure that the exposure concentration reached the expected level, the external exposure concentration of BaP in the exposure cabinet was regularly monitored by using the activated carbon tube method. Four TH-150C mid-flow samplers were used to start the collection at the same time around the exposure cabinet, and the samples were collected with the GH-1 type activated carbon tube. , all samples were collected at a flow rate of 0.5L / min for 2 hours, and the door of the poisoning cabinet was closed during sampling to ensure a relatively closed environment. Immediately after the samples were collected, the activated carbon tubes were sealed and stored in a freezer at -20°C. GC-MSD (Agilent 6890 5973 7683 autosampler) was used to analyze the content of BaP in the collected gas samples.

[0041] By measuring the BaP standard series solutions (0, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5mg / L), the peak area of ​​the standard seri...

Embodiment 3

[0046] The construction of embodiment three mouse lung cancer model

[0047] A method for constructing a mouse lung cancer model, comprising the steps of:

[0048] In S1, C57BL / 6J wild-type mice were taken, and after 1 week of adaptation, they were placed in the main cabinet of the dynamic inhalation exposure cabinet for benzopyrene exposure. The conditions and parameters of the exposure included: 5 days / week, 6h / day, The exposure concentration of pyrene is 10.294±0.518μg / m 3 , a temperature of 22°C, a relative humidity of 60%, and an oxygen content of 20%; during the exposure period, the mice drank water freely and stopped feeding; the main cabinet maintained a negative pressure of 0.08 MPa, and the mechanical ventilation system maintained a ventilation frequency of 12 times / h;

[0049] After S2 exposure for 180 days, a mouse lung cancer model was obtained.

[0050] After exposure for 180 days, the mice were anesthetized with ether, and the whole lung tissue was surgically ...

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Abstract

The invention provides a construction method of a mouse lung cancer model. The method includes the steps of: S1, taking C57BL / 6J wild-type mice of 6-8 weeks old, performing adaptation for 1-2 weeks, then putting the mice into a main cabinet of an inhalation exposure cabinet for benzopyrene exposure with the condition parameters of: 5d / week, 5-8h / day, a benzopyrene exposure concentration of 9-12microg / m<3>, and stopping feeding of the mice and allowing free drinking during the exposure; and S2, performing exposure for 23-28 weeks, thus obtaining the mouse lung cancer model. The invention belongs to the field of biotechnology. The method provided by the invention causes no artificial damage to the original structure of the lung, also does not cause infection or other interferences, conformsto the actual environmental exposure way of the human body, can better reflect the actual occurrence of lung cancer in the human body, and is beneficial to the development of lung cancer-related therapeutic drugs.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for constructing a mouse lung cancer model. Background technique [0002] Lung cancer, as one of the most common malignant tumors with the highest mortality rate in the world, seriously threatens human life and health. With the acceleration of industrialization and urbanization in my country, the harm of lung cancer is increasing day by day, and it has become the malignant tumor with the highest morbidity and mortality in my country. As lung cancer has increasingly become a malignant tumor disease with high incidence, lung cancer has caused a huge burden on people's life and economy, and has become a serious public health problem. The clinical manifestations of lung cancer are complicated, and it is difficult to detect and diagnose early, which makes it difficult to improve the efficacy of lung cancer. Since the human body cannot be directly used for experiments, the estab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/015
CPCA61K9/007A61K31/015
Inventor 黄海燕付英斌吴德生肖云军刘建军
Owner SHENZHEN CENT FOR DISEASE CONTROL & PREVENTION
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