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3D printing Ti-hydrogel-osteoblast bone tissue engineering support and preparation method thereof

A technology of bone tissue engineering and 3D printing, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of allergic reaction, unguaranteed biological safety, tumor causing, etc., and achieve good mechanical properties and good tissue integration. Properties and effects on biosafety

Inactive Publication Date: 2018-11-23
南京冬尚生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, biologically active factors have the possibility of immune rejection, pathogen transmission, allergic reaction, potential tumorigenicity, and teratogenicity, and biological safety cannot be guaranteed, and clinical-grade biological factors such as rhBMP-2 and TGF-β often need to be imported, and The price is expensive, and the purchase price per milligram reaches nearly a thousand dollars

Method used

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  • 3D printing Ti-hydrogel-osteoblast bone tissue engineering support and preparation method thereof
  • 3D printing Ti-hydrogel-osteoblast bone tissue engineering support and preparation method thereof
  • 3D printing Ti-hydrogel-osteoblast bone tissue engineering support and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Step 1: Prepare 3D printed Ti scaffold

[0040] Firstly, the Ti6L4V powder was sintered into shaped fiber bundles by laser sintering (Selective Laser Sintering, SLM) technology, and the fiber bundles were arranged and spliced ​​at 0-90°, 0-60-120°, etc. to prepare 3D printed Ti scaffolds ( figure 2 , 3 );

[0041] Step 2: Osteoblast extraction and culture

[0042] Take the calvaria of young white rabbits, cut it into pieces, add 5ml of 0.25% EDTA trypsin, digest for 30min, centrifuge to remove the supernatant, digest with 10ml of 0.1% type II collagenase for 4h, take the supernatant and centrifuge to remove the supernatant, culture medium Suspend the cells and inoculate, culture, change the medium after the cells adhere to the wall, carry out Alizarin red staining and ALP staining identification, and take 5x10 cells after amplification to the third generation 4 / ml cells for use;

[0043] Step 3: Preparation of hydrogel carrying osteoblasts:

[0044] Take agarose ...

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Abstract

The invention discloses a 3D printing Ti-hydrogel-osteoblast bone tissue engineering support and a preparation method thereof. The method comprises the following steps of enabling Ti6Al4V powder to beprocessed into a 3D printing Ti support with a porous structure by utilizing SLM technology; then filling a hydrogel-osteoblast mixture in the pores of the 3D printing Ti support under a certain condition so as to prepare a 3D printing Ti-hydrogel-osteoblast bone defect repair support. The support has the advantages that the structure is simple and reliable, the shape and microstructure are controllable, the mechanical property is reliable, the implantation is convenient, the trauma is small, and the cost is low.

Description

technical field [0001] The invention belongs to the field of bone tissue repair and reconstruction, and relates to a 3D printed Ti-hydrogel-osteoblast bone tissue engineering scaffold and a preparation method thereof. Background technique [0002] The repair and reconstruction of bone defects is one of the clinical problems in orthopedics. It is well known that autogenous bone is considered the gold standard for bone grafting, but its source is limited and may lead to complications such as necrosis of the supply site, postoperative chronic pain, allergic reaction, and infection. While allograft bone and xenograft bone are widely used because they come from a wide range of sources and do not require additional surgical operations, but there are also complications such as slow integration and remodeling, immune rejection, and disease transmission, especially reducing the osteoinductive and osteoconductive. [0003] At present, scaffold materials include bioactive calcium pho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/06A61L27/38A61L27/50A61L27/52A61L27/56A61L27/58
CPCA61L27/06A61L27/3821A61L27/3847A61L27/50A61L27/52A61L27/56A61L27/58A61L2430/02
Inventor 王黎明姚庆强徐燕胡军
Owner 南京冬尚生物科技有限公司
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