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Bacillus subtilis and method for preparing negundo chastetree fruit microecological preparation from bacillus subtilis

A technology of Bacillus subtilis and micro-ecological preparations, applied in the field of microbial engineering, can solve rare problems, achieve the effects of promoting growth and development, improving the activity of beneficial bacteria, and improving feed utilization

Active Publication Date: 2018-11-23
TONGREN POLYTECHNIC COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some reports of adopting Bacillus subtilis to veterinary medicine at present, but the report of adopting Bacillus subtilis semi-solid fermentation to prepare Chinese veterinary medicine is still rare

Method used

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  • Bacillus subtilis and method for preparing negundo chastetree fruit microecological preparation from bacillus subtilis
  • Bacillus subtilis and method for preparing negundo chastetree fruit microecological preparation from bacillus subtilis
  • Bacillus subtilis and method for preparing negundo chastetree fruit microecological preparation from bacillus subtilis

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A kind of Bacillus subtilis (Bacillus subtilis) S21, described Bacillus subtilis (Bacillus subtilis) S21 was preserved on April 02, 2018 in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the strain preservation number is CGMCCNo: 15544; The Bacillus subtilis (Bacillus subtilis) S21 can be used to produce surfactin, fengycin, protease, lipase, amylase and cellulase; the Bacillus subtilis (Bacillus subtilis) S21 can be used alone or in combination with other beneficial bacteria to ferment and produce Chinese veterinary medicinal materials .

[0041] A method for preparing Vitex vetex probiotics using the above-mentioned bacillus subtilis, comprising the following steps:

[0042] (1) Culture substrate preparation: get 80 parts of Vitex vetex dry powder, 5 parts of soybean powder, 3 parts of glucose, 2 parts of urea and 12 parts of water, mix and stir evenly, and obtain the culture medium substrate through high-temperat...

Embodiment 2

[0047] A kind of Bacillus subtilis (Bacillus subtilis) S21, described Bacillus subtilis (Bacillus subtilis) S21 was preserved on April 02, 2018 in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the strain preservation number is CGMCCNo: 15544; The Bacillus subtilis (Bacillus subtilis) S21 can be used to produce surfactin, fengycin, protease, lipase, amylase and cellulase; the Bacillus subtilis (Bacillus subtilis) S21 can be used alone or in combination with other beneficial bacteria to ferment and produce Chinese veterinary medicinal materials .

[0048] A method for preparing Vitex vetex probiotics using the above-mentioned bacillus subtilis, comprising the following steps:

[0049] (1) Culture substrate preparation: get 85 parts of Vitex vetex dry powder, 10 parts of soybean powder, 5 parts of glucose, 4 parts of urea and 18 parts of water, mix and stir evenly, and obtain the culture medium substrate through high-tempera...

Embodiment 3

[0054] A kind of Bacillus subtilis (Bacillus subtilis) S21, described Bacillus subtilis (Bacillus subtilis) S21 was preserved on April 02, 2018 in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the strain preservation number is CGMCCNo: 15544; The Bacillus subtilis (Bacillus subtilis) S21 can be used to produce surfactin, fengycin, protease, lipase, amylase and cellulase; the Bacillus subtilis (Bacillus subtilis) S21 can be used alone or in combination with other beneficial bacteria to ferment and produce Chinese veterinary medicinal materials .

[0055] A method for preparing Vitex vetex probiotics using the above-mentioned bacillus subtilis, comprising the following steps:

[0056] (1) Culture substrate preparation: get 81 parts of Vitex vetex dry powder, 6 parts of soybean powder, 3.5 parts of glucose, 2.5 parts of urea and 13 parts of water, mix and stir evenly, and obtain the culture medium matrix through high-tempera...

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Abstract

The invention discloses bacillus subtilis, and particularly relates to bacillus subtilis S21. The bacillus subtilis S21 is preserved in CGMCC (China General Microbiological Culture Collection Center)on April 2, 2018. The strain preservation number is CGMCC No:15544. The invention also discloses a concrete method for preparing traditional Chinese veterinary medicine of a negundo chastetree fruit microecological preparation from the strain through semi-solid fermentation. The traditional Chinese veterinary medicine microecological preparation is developed; the spore number is high; the bacteriostat activity is high; the activity of protease, lipase, amylase and cellulase is high; the bacillus subtilis can be applied to no-resistance culture of animal husbandries; the disease prevention andcontrol prevention can be better achieved; the goal of promoting the growth and development of the livestock is achieved; good market prospects are realized.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and relates to a bacillus subtilis and a method for preparing Vitex vetex microecological preparations. Background technique [0002] The large-scale application of antibiotics in the aquaculture industry has a positive effect on animal breeding, but it also has negative effects, such as antibiotic residues and safety issues arising from drug-resistant bacteria. Therefore, finding alternatives to antibiotics has become the focus and focus of scientific research. A large number of studies have shown that Chinese veterinary drug microecological preparations prepared with Chinese medicinal materials as the main fermentation substrate play an important role in antibiotic-free breeding. The micro-ecological preparation of Chinese veterinary medicine combines the effects of Chinese medicinal materials and beneficial bacteria, which can not only give full play to the efficacy of the tradit...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K10/12C12R1/125
CPCA23K10/12A23K10/18C12N1/20C12R2001/125C12N1/205
Inventor 胡美忠郁建生刘杰陈敏张新卓
Owner TONGREN POLYTECHNIC COLLEGE