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Mycotoxin colorimetric sensing method based on fluorescent metal-organic framework material

A metal-organic framework and mycotoxin technology, applied in the field of agricultural product and food quality testing, can solve the problems of poor water stability and unfavorable rapid detection of mycotoxins, and achieve the effects of good dispersion, improved sensitivity and uniform particle size

Inactive Publication Date: 2018-11-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the coordination bond formed by this method has poor stability in water, which is not conducive to the rapid detection of mycotoxins on site.

Method used

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  • Mycotoxin colorimetric sensing method based on fluorescent metal-organic framework material
  • Mycotoxin colorimetric sensing method based on fluorescent metal-organic framework material
  • Mycotoxin colorimetric sensing method based on fluorescent metal-organic framework material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 175mg ZrCl 4 , 125mg H 4 TCPB ligand and 6.3g structure regulator benzoic acid were dissolved in 40mL DMF solution. The mixed solution was ultrasonicated for 15 min to mix the solution thoroughly. The dispersion liquid was packaged in a 100 mL quartz reactor, and a cloudy liquid containing Zr-TCPB was obtained after continuous heating at 120° C. for 24 hours. After natural cooling at room temperature, wash with methanol centrifugation three times at a centrifugation speed of 6000 rpm. Place the washed product in CH 2 Cl 2 for 24 hours to replace the remaining DMF molecules in the pores. After centrifuging again, the product was dried in an oven at 60° C. for 24 hours to obtain a white powder, which was the activated fluorescent Zr-TCPB material. Appearance: as figure 1 As shown, scanning electron microscopy and transmission electron microscopy can be used to see that the prepared nanomaterials are rod-like structures with a size of 1 μm. Further observations show...

Embodiment 2

[0090] Add AFB to walnut dew sold on the market 1 , to verify the application of this colorimetric method in actual detection. Add 100uL 1mM AFB to 10mL walnut dew 1 Methanol solution to make 10μM AFB 1 walnut dew, and then serially diluted to 10 μM, 1 μM and 0.1 μM spiked samples. Adopt embodiment 1 to prove the detection method in 1 to the AFB in the standard sample 1 to test. As shown in Table 1, by demonstrating with Example 1 that AFB in 1 1 Comparing the standard response curves, the calculated concentrations of the 0.1 μM, 1 μM and 10 μm spiked samples were 0.0975, 0.079 and 9.73 μM, respectively. By comparing with the standard detection method high performance liquid chromatography (HPLC) ( Figure 9 ), Figure 9 (a), (b), and (c) are the detection results of 0.1 μM, 1 μM, and 10 μM aflatoxin B1 in walnut milk, respectively; (d), (e), and (f) are 0.1 μM, 1 μM in almond milk, respectively , 10μM aflatoxin B1 detection results.

[0091] Figure 9 It can be seen...

Embodiment 3

[0095] Add AFB to almond milk sold in the market 1 , to verify the application of this method in practical detection. Add 100uL 1mM AFB to 10mL almond milk 1 Methanol solution to make 10μM AFB 1 walnut dew, and then serially diluted to 10 μM, 1 μM and 0.1 μM spiked samples. Adopt embodiment 1 to prove the detection method in 1 to the AFB in the standard sample 1 to test. As shown in Table 1, by demonstrating with Example 1 that AFB in 1 1 Comparing the standard response curves, the calculated concentrations of 0.1 μM, 1 μM and 10 μM spiked samples were 0.0966, 1.041, and 9.06, respectively. By comparing with the standard detection method high performance liquid chromatography (HPLC) ( Figure 9), it was found that the reproducibility rates of the standard sample added by this method were 102.1, 108.8 and 93.3 respectively, which had a high reproducibility rate and could meet the requirements of rapid and sensitive detection in practical applications.

[0096] In summary...

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Abstract

The invention discloses a mycotoxin colorimetric sensing method based on fluorescent metal-organic framework material. The fluorescent metal-organic framework material is synthesized by a hydrothermalmethod, and dissolved in water to form a test solution after activation; after the test solution is uniformly mixed with different solutions to be tested, emission spectra of mycotoxins are measuredby a microplate reader to establish a response relation model between the different concentrations of each type of mycotoxins and the intensity of the emission spectra; after a practical sample is uniformly mixed with the test solution, the emission spectra of the mycotoxins in the practical sample is measured by the microplate reader, and the emission spectra are compared and tested according tothe relation model to obtain the concentration. The method has high specific surface area, good water stability while responding to a variety of mycotoxins. The preparation method has the advantages of easy operation, low cost, high sensitivity and test limit up to ppb level, and has a broad application prospect in the sensing field.

Description

technical field [0001] The invention belongs to the field of agricultural products and food quality detection, relates to a new method for rapid detection of carcinogens therein, and in particular relates to a colorimetric sensing method for mycotoxins based on fluorescent metal organic framework materials. Background technique [0002] Mycotoxins are poisonous and harmful substances produced by molds or fungi. They can enter human and animal body through feed or food, cause acute or chronic toxicity to human and animal, and damage the liver, kidney, nerve tissue, hematopoietic tissue and skin tissue of the body. Among them, aflatoxins are a type of highly toxic mycotoxins produced by the secondary metabolism of filamentous fungi such as Aspergillus flavus. They have strong carcinogenic and teratogenic properties and have been reported to be found in a variety of moldy agricultural products, such as peanuts. , corn, rice, milk, etc., have aroused widespread concern in socie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 李延斌李志尚傅迎春
Owner ZHEJIANG UNIV
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