Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for extracting polyphenol substance from new variety oil peony seed shells

A technology of polyphenols and new varieties, which is applied in the field of extracting polyphenols from new varieties of oily peony seed shells, can solve the problems of waste of resources, pollution of the environment by oily peony seed shells, etc., reduce the amount of organic solvents, and shorten the extraction time , the effect of solving environmental pollution problems

Active Publication Date: 2018-11-30
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
View PDF1 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It not only solves the problems of polluting the environment and waste of resources from the oil peony seed shell, but also develops the utilization value of peony

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting polyphenol substance from new variety oil peony seed shells
  • Method for extracting polyphenol substance from new variety oil peony seed shells
  • Method for extracting polyphenol substance from new variety oil peony seed shells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] The method for extracting polyphenols from the shell of the new variety Paeoniae oleifera of the present embodiment, the steps are as follows:

[0069] (1) Take 3g of peony seed shell powder, add 75ml of 80% ethanol solution, put it into ultrasonic extraction for 20min, filter, extract the filter residue together with filter paper with ethanol solution of the same concentration, combine the filtrate, and put the filtrate into a sample bottle spare;

[0070] (2) Take 2ml of the sample solution in step (1) into a 10ml graduated centrifuge tube, add 3ml of deionized water, add 0.1ml of fatty alcohol polyoxyethylene ether AEO-9 and 0.3ml of n-hexanol into the centrifuge tube, vortex for 2min , centrifuge at 3000r / min for 15min, and the target substance is extracted on the upper layer of the centrifuge tube;

[0071] (3) According to the above detection method of polyphenols, it was detected by ultraviolet spectrophotometry, and the extraction amount of polyphenols was calc...

Embodiment 2

[0073] The method for extracting polyphenols from the shell of the new variety Paeoniae oleifera of the present embodiment, the steps are as follows:

[0074] (1) Take 3g of peony seed shell powder, add 75ml of 80% ethanol solution, put it into ultrasonic extraction for 20min, filter, extract the filter residue together with filter paper with ethanol solution of the same concentration, combine the filtrate, and put the filtrate into a sample bottle spare.

[0075] (2) Take 2ml of the sample solution in step (1) in a 10ml graduated centrifuge tube, add 3ml of deionized water, add 0.2ml of fatty alcohol polyoxyethylene ether AEO-9 and 0.5ml of n-hexanol in the centrifuge tube, vortex for 2min , centrifuged at 3000r / min for 15min, and the target substance was extracted on the upper layer of the centrifuge tube.

[0076] (3) According to the above-mentioned detection method of polyphenols, it was detected by ultraviolet spectrophotometry, and the extraction amount of polyphenols wa...

Embodiment 3

[0078] The method for extracting polyphenols from the shell of the new variety Paeoniae oleifera of the present embodiment, the steps are as follows:

[0079] (1) Take 3g of peony seed shell powder, add 75ml of 80% ethanol solution, put it into ultrasonic extraction for 20min, filter, extract the filter residue together with filter paper with ethanol solution of the same concentration, combine the filtrate, and put the filtrate into a sample bottle spare.

[0080] (2) Take 2ml of the sample solution in step (1) in a 10ml graduated centrifuge tube, add 3ml of deionized water, add 0.3ml of fatty alcohol polyoxyethylene ether AEO-9 and 0.9ml of n-hexanol in the centrifuge tube, vortex for 2min , centrifuged at 3000r / min for 15min, and the target substance was extracted on the upper layer of the centrifuge tube.

[0081] (3) According to the above polyphenol detection method, the ultraviolet spectrophotometry is used for detection, and the calculated polyphenol extraction amount ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting polyphenol substance from new variety oil peony seed shells. The method comprises the following steps that extraction of herbaceous peony seed shell powder is carried out by adopting assistance of ultrasonic wave and ethanol solution, extracting solution is concentrated and dried, polyphenol content is measured, three factors of ethanol content, liquid and materials ratio and extracting time are selected to carry out a single factor experiment, a three-factor and three-level experimental design is carried out according to a Box-Benhnken design principle, an optimum extracting process parameter is determined; and extract liquor is obtained by using the extracting process parameter, nonionic surface active agent and assisted extracting agent are added in the extract liquor, and vortex and centrifugation are carried out. According to the method for extracting the polyphenol substance from the new variety oil peony seed shells, a method of combining ultrasonic wave extracting and cloud point extracting is used, compared with an optimized method for extracting ultrasonic wave polyphenol substance, the method for extracting the polyphenol substance from the new variety oil peony seed shells has the characteristics that extracting ratio is high up to 1.5 times, extracting condition is gentle, the extracting agent is friendly to the environment, and extracting cost is low. Waste oil peony seed shells are recycled, not only the waste oil peony seed shells can be made profitable, but also environmental pollution problems can be greatlysolved.

Description

technical field [0001] The invention belongs to the field of extracting plant components, and in particular relates to a method for extracting polyphenols from the shell of a new variety of oily peony seed. Background technique [0002] Peony is a traditional Chinese medicine used to treat various diseases. It can be divided into red peony and white peony, both of which use roots as medicine. The white peony does not set seeds or the seeds are very small; Low. In industry, the effective active ingredients are generally extracted from the rhizome of Paeoniae Radix. Youshao is a new variety bred by crossing Chishao and Hangshao for more than 20 years. It not only has a well-developed root system, but also has a breakthrough in seed production. The seed yield of Paeoniae suffolius over 4 years is not less than 450 catties / mu. Even more than 800 catties per mu. After experimental analysis, peony seed oil contains more than 95% unsaturated fatty acids beneficial to the human bo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/71A61K36/65A61K131/00
CPCA61K36/65A61K36/71A61K2236/333
Inventor 时桂芹任菲谢小东尹士乐韩亚伟
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY