One-step decalcification, deproteinization and defat extraction method for chitin from crab shell
A chitin and fat-removing technology, which is applied in the field of deproteinization, defat extraction of chitin, and one-step decalcification from crab shells, can solve the problems of increasing steps and production costs, avoid multiple transfers, and improve extraction efficiency Effect
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Embodiment 1
[0023] Embodiment 1: The discarded crab shells are washed with clear water and drained. Weigh 5 parts of cleaned crab shells 50g, place them in 1000mL beakers respectively, stir and react at room temperature for 12h according to the solid-liquid ratio of 1:10 (g / mL), add 5%, 7.5%, 10%, 12.5% %, 15%, and 20% sulfonic acid solutions, replace the sulfonic acid solution once midway, wash the obtained sample with 50°C water to remove residual sulfonic acid, filter and dry to obtain chitin; then use 200mL of 3% Soak the above-mentioned chitin in potassium permanganate solution for 30 minutes to decolorize, drain the water, soak in oxalic acid solution and stir until the chitin is white, then wash with water to remove residual potassium permanganate and oxalic acid, filter and dry to obtain white chitin. The ash content and nitrogen content in chitin extracted under different sulfonic acid concentrations are shown in figure 1 .
Embodiment 2
[0024] Embodiment 2: The discarded crab shells are washed with clear water and drained. Weigh 5 parts of cleaned crab shells, 50g, and place them in 1000mL beakers respectively. Under the conditions of sulfonic acid concentration of 10%, solid-to-liquid ratio of 1:10 (g / mL), and stirring for 12 hours, respectively, at 25°C, 40°C, React at 50°C, 60°C, and 70°C, replace the sulfonic acid solution once in the middle, wash the obtained sample with 50°C water to remove residual sulfonic acid, filter, and dry to obtain chitin; then use 200mL of 3% permanganese Soak the above-mentioned chitin in potassium permanganate solution for 30 minutes to decolorize, drain the water, soak in oxalic acid solution and stir until the chitin turns white, then wash with water to remove residual potassium permanganate and oxalic acid, filter and dry to obtain white chitin. Ash content and nitrogen content in chitin extracted at different temperatures see figure 2 .
Embodiment 3
[0025] Embodiment 3: The discarded crab shells are washed with clear water and drained. Weigh 50g of 7 cleaned crab shells, place them in 1000mL beakers respectively, and stir at constant temperature for 4h, 8h, 12h, 16h under the conditions of sulfonic acid concentration 10%, solid-liquid ratio 1:10 (g / mL) and 50°C , 20h, 24h, 28h, replace the sulfonic acid solution once in the middle, wash the obtained sample with 50 ℃ water to remove the residual sulfonic acid, filter and dry to obtain chitin; then soak it in 200mL of 3% potassium permanganate solution The above-mentioned chitin was decolorized for 30 minutes, drained, soaked in oxalic acid solution and stirred until the chitin turned white, then washed with water to remove residual potassium permanganate and oxalic acid, filtered and dried to obtain white chitin. Ash content and nitrogen content in chitin extracted under different reaction times are shown in image 3 .
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