Brewing yeast ST28-61 screening and application in beer brewing thereof

A technology for Saccharomyces cerevisiae and beer, applied in the biological field, can solve the problems affecting the economic benefit of beer enterprises, slow reduction of diacetyl, long fermentation cycle, etc. Effect

Inactive Publication Date: 2018-11-30
FULLARTON BIOENG TECH BEIJING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, my country uses the following fermenting yeast, that is, grape juice yeast (S.uvaram) to brew beer. Its traditional fermentation cycle is long, post-fermentation diacetyl reduction is slow, and the wine age is long, which affects the economic benefits of beer companies.

Method used

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  • Brewing yeast ST28-61 screening and application in beer brewing thereof
  • Brewing yeast ST28-61 screening and application in beer brewing thereof
  • Brewing yeast ST28-61 screening and application in beer brewing thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1, Screening of strains with excellent fermentation performance of Saccharomyces cerevisiae

[0039] (1) Isolation and purification of bacterial strains

[0040] 1. Strains

[0041] Space Saccharomyces cerevisiae (Saccharomyces cerevisiae) ST28: The space mutagenic strain returned by Saccharomyces cerevisiae GT28 on the ground via Tiangong-2 and Shenzhou-11 spacecraft, preserved in Fullerton Bioengineering Technology (Beijing) Co., Ltd. Aerospace microbial strain bank. In order to distinguish the strain difference before and after space launch, the space mutagenized strain after ground Saccharomyces cerevisiae GT28 was labeled as space Saccharomyces cerevisiae ST28.

[0042] 2. Strain activation

[0043] Put the ground Saccharomyces cerevisiae GT28 and space Saccharomyces cerevisiae ST28 glycerin preservation tubes frozen in the -80°C freezer into 5mL YEPD liquid medium at 2% to 3% (volume percentage), culture at 28°C for 22 to 24 hours, and so on. The third...

Embodiment 2

[0083] Embodiment 2, beer fermentation test of space Saccharomyces cerevisiae ST28-61 strain

[0084] In this embodiment, the unit % of the inoculum amount is volume percentage.

[0085] (1) Determination of optimal inoculum volume and transplantation / cultivation time of ST28-61 strain

[0086] Put the storage tube of Saccharomyces cerevisiae ST28-61 strain frozen in the -80°C freezer into a test tube containing 5mL liquid YEPD medium at 2% to 3%, and culture it at 28°C for 22 to 24 hours, so that it can be activated continuously for 3 generations Afterwards, press 2%, 3%, 4%, 5%, and 10% inoculum amounts into test tubes containing 10 mL of liquid YEPD medium respectively (2%, 3%, 4%, 5%, and 10% inoculum amounts were The inoculation amount of live bacteria was 6.2×10 6 CFU / mL, 9.6×10 6 CFU / mL, 1.28×10 7 CFU / mL, 1.6×10 7 CFU / mL, 3.2×10 7 CFU / mL), cultured at 28°C for 24h. Under the wavelength 560nm, respectively measure and record the OD value (optical density value) of...

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Abstract

The invention discloses brewing yeast ST28-61 screening and application in beer brewing thereof. The preservation number of the brewing yeast in China General Microbiological Culture Collection Centeris CGMCC No.16029. The brewing yeast is used for beer brewing. Beer brewed by the brewing yeast is low in diacetyl yield and high in alcohol yield. Wine age can be shortened, fermentation capacity ishigh, alcohol tolerance is high, anti-corrosion ability of hops (yeast fermentation is not inhibited by hops) is high. Diacetyl yield in the main fermentation process is lower than the national standard gb4927-2008, and therefore the post-fermentation process is not required, and the problem that the current fermentation period of beer brewing in China is relatively long is solved.

Description

technical field [0001] The invention relates to the screening of Saccharomyces cerevisiae ST28-61 and its application in brewing beer in the field of biotechnology. Background technique [0002] Saccharomyces cerevisiae is a single-celled eukaryotic microorganism that mainly reproduces by budding. The cell shape is oval or long oval (ellipse). The colony size on the YEPD medium plate is 4-5 mm, and the surface is smooth and convex. From the beginning, the surface of the aging is radioactive umbrella-shaped, the texture is moist, and the color is milky white. When fermented in wort liquid medium, air bubbles and foams are produced on the surface, and the bacteria are suspended in the medium and appear turbid. In the later stage of fermentation, the bacteria cells are deposited on the bottom of the container, and the fermentation broth becomes clear. Excellent Saccharomyces cerevisiae can ferment grape juice at 25-30°C to produce good fruity and bouquet. Its optimum fermenta...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12C11/00C12R1/865
CPCC12C11/00C12N1/18C12N1/185C12R2001/865
Inventor 郝红炜刘慧张红星谢远红
Owner FULLARTON BIOENG TECH BEIJING CO LTD
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