Brewing yeast ST28-61 screening and application in beer brewing thereof
A technology for Saccharomyces cerevisiae and beer, applied in the biological field, can solve the problems affecting the economic benefit of beer enterprises, slow reduction of diacetyl, long fermentation cycle, etc. Effect
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Embodiment 1
[0038] Example 1, Screening of strains with excellent fermentation performance of Saccharomyces cerevisiae
[0039] (1) Isolation and purification of bacterial strains
[0040] 1. Strains
[0041] Space Saccharomyces cerevisiae (Saccharomyces cerevisiae) ST28: The space mutagenic strain returned by Saccharomyces cerevisiae GT28 on the ground via Tiangong-2 and Shenzhou-11 spacecraft, preserved in Fullerton Bioengineering Technology (Beijing) Co., Ltd. Aerospace microbial strain bank. In order to distinguish the strain difference before and after space launch, the space mutagenized strain after ground Saccharomyces cerevisiae GT28 was labeled as space Saccharomyces cerevisiae ST28.
[0042] 2. Strain activation
[0043] Put the ground Saccharomyces cerevisiae GT28 and space Saccharomyces cerevisiae ST28 glycerin preservation tubes frozen in the -80°C freezer into 5mL YEPD liquid medium at 2% to 3% (volume percentage), culture at 28°C for 22 to 24 hours, and so on. The third...
Embodiment 2
[0083] Embodiment 2, beer fermentation test of space Saccharomyces cerevisiae ST28-61 strain
[0084] In this embodiment, the unit % of the inoculum amount is volume percentage.
[0085] (1) Determination of optimal inoculum volume and transplantation / cultivation time of ST28-61 strain
[0086] Put the storage tube of Saccharomyces cerevisiae ST28-61 strain frozen in the -80°C freezer into a test tube containing 5mL liquid YEPD medium at 2% to 3%, and culture it at 28°C for 22 to 24 hours, so that it can be activated continuously for 3 generations Afterwards, press 2%, 3%, 4%, 5%, and 10% inoculum amounts into test tubes containing 10 mL of liquid YEPD medium respectively (2%, 3%, 4%, 5%, and 10% inoculum amounts were The inoculation amount of live bacteria was 6.2×10 6 CFU / mL, 9.6×10 6 CFU / mL, 1.28×10 7 CFU / mL, 1.6×10 7 CFU / mL, 3.2×10 7 CFU / mL), cultured at 28°C for 24h. Under the wavelength 560nm, respectively measure and record the OD value (optical density value) of...
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