Unlock instant, AI-driven research and patent intelligence for your innovation.

Prediction method of collision energy required for quantifying the content of triterpene saponins in traditional Chinese medicine based on electrospray protonation cracking

A technology of triterpenoid saponins and prediction methods, which can be applied to measuring devices, instruments, scientific instruments, etc., and can solve problems such as time-consuming and labor-intensive

Active Publication Date: 2021-05-11
夏永刚
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the early stage, the inventor's research group optimized CE through traditional syringe pump method, single factor experiment method and response surface method. However, when multiple triterpenoid saponins are analyzed at the same time, it is time-consuming and laborious to use these methods to obtain the optimal collision energy. Therefore, there is an urgent need to provide a simple and accurate prediction method for the collision energy required for quantifying the content of triterpene saponins in traditional Chinese medicine based on electrospray protonation cracking

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Prediction method of collision energy required for quantifying the content of triterpene saponins in traditional Chinese medicine based on electrospray protonation cracking
  • Prediction method of collision energy required for quantifying the content of triterpene saponins in traditional Chinese medicine based on electrospray protonation cracking
  • Prediction method of collision energy required for quantifying the content of triterpene saponins in traditional Chinese medicine based on electrospray protonation cracking

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0035] Experimental Example 1 Quantitative ideal collision energy prediction experiment of 85 triterpenoid saponins based on protonated splitting ion pairs

[0036] 1 Experimental instrument

[0037] Qtrap-4000 triple quadrupole linear ion trap mass spectrometer (AB SCIEX, USA); H-class ultra-high performance liquid chromatograph (on-line degasser, autosampler, quaternary pump, Waters, USA); ACQUITY A UPLC HSS T3 chromatographic column (2.1×150 mm, 1.8 μm) was connected to a Waters HSS T3 guard column (2.1×5 mm, 1.8 μm). Milli-Q water purifier (Millipore China Co., Ltd.), ML104 / 02 Electronic Analytical Balance (Mettler Toledo Instruments Shanghai Co., Ltd.), Vortex 3000 Vortex Oscillator (Wiggens, Germany); Mikro 200R Centrifuge Machine (Hettich Company, Germany), KQ-500DB CNC Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0038] 2 Preparation of standard products

[0039] Precisely weigh the triterpenoid saponins reference substance 1-85 (structure as fi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for predicting the collision energy required for quantifying the content of triterpene saponins in traditional Chinese medicines based on electrospray protonation and cracking. plants; (2) select representative triterpene saponin model compounds; (3) quantify the content of triterpene saponins in medicinal plants based on electrospray protonation cleavage; use the following equation to predict the required collision energy: CE=0.0337*Q 1 +0.5686, Q 1 =[M+H / NH 4 ] + , Q 1 Linear range 600‑1600Da, R 2 =0.91; CE=0.0337M w +0.9361, M w Linear range 600‑1600Da, R 2 = 0.9096. There is no significant difference between the CE value obtained by the prediction method of the present invention and the CE value obtained by the single factor investigation method, indicating that the prediction method of the present invention can be applied to the detection of triple quadruple mass spectrometry.

Description

technical field [0001] The invention relates to a method for predicting the collision energy required for analyzing and quantifying the content of triterpenoid saponins in traditional Chinese medicines, in particular to a method for rapidly predicting the ideal collision energy required for quantifying triterpene saponins in traditional Chinese medicines based on electrospray protonation cracking-multiple reaction monitoring mass spectrometry, The invention belongs to the field of quantitative detection of the content of triterpenoid saponins in traditional Chinese medicines. Background technique [0002] Triterpene saponins form stable [M+H] in mobile phases containing formic acid or ammonium formate + and / or [M+NH 4 ] + Molecular ion peaks, using liquid chromatography-tandem quadrupole mass spectrometer, using electrospray cation tandem mass spectrometry detection mode (ESI + -MRM), identified predictable MRM-targeted transitions with Q 1 and Q 3 The calculation formu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 夏永刚梁军郭信东宋艳
Owner 夏永刚