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A method for separating and purifying phosphatidylcholine by polyionic liquid extraction

A technology of polyionic liquid and phosphatidylcholine is applied in the field of chemical engineering to achieve the effects of high yield, good biocompatibility and simple operation

Active Publication Date: 2021-01-08
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] ZL 201310169075.8 and ZL 2013 10170809.4 disclose a method of extracting phosphatidylcholine with solvents such as ionic liquids as the extraction agent, which has the characteristics of low cost and high purity, but some ionic liquids have certain safety and biocompatibility problems

Method used

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  • A method for separating and purifying phosphatidylcholine by polyionic liquid extraction
  • A method for separating and purifying phosphatidylcholine by polyionic liquid extraction
  • A method for separating and purifying phosphatidylcholine by polyionic liquid extraction

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Commercially available soybean phospholipid mixture (the weight percentage of phosphatidylcholine: 28%) and normal hexane to make phospholipid mixture concentration is the stock solution of 260 g / liter, with poly(1-vinyl-3-ethylimidazole The ethanol solution of bromide) is the extractant (the weight percentage of poly(1-vinyl-3-butylimidazolium bromide): 12%), with n-hexane as the washing agent, at 30°C, in the fractionation extraction device Fractional distillation extraction was carried out, and the extract was collected. The extract is back-extracted with n-hexane, and the n-hexane solution is vacuum-concentrated to remove the organic solvent and dried to obtain the phosphatidylcholine product. Through HPLC analysis, the absolute purity of phosphatidylcholine in the final product was 76.2%, and the yield was 91.8%.

Embodiment 2

[0058] Commercially available soybean phospholipid mixture (the weight percentage of phosphatidylcholine: 28%) and n-heptane are made into the raw material solution that phospholipid mixture concentration is 200 g / liter, and poly(1-vinyl-3-octyl The methanol solution of imidazolium chloride salt) is the extractant (the weight percentage of poly(1-vinyl-3-octyl imidazolium chloride salt): 20%), with n-heptane as the washing agent, at 25°C, in fractional distillation Fractional extraction is carried out in the extraction device, and the extract is collected. The extract is back-extracted with n-heptane, and the n-heptane solution is vacuum-concentrated to remove the organic solvent and dried to obtain the phosphatidylcholine product. Through HPLC analysis, the absolute purity of phosphatidylcholine in the final product was 81.2%, and the yield was 95.8%.

Embodiment 3

[0060] Commercially available soybean phospholipid mixture (weight percentage of phosphatidylcholine: 42%) and petroleum ether with a boiling range of 90 to 120° C. are made into a raw material solution with a concentration of phospholipid mixture of 280 grams per liter, and poly(vinyl tri The methanol solution of butylammonium acetate) is the extractant (the weight percentage of poly(vinyl tributylammonium acetate): 25%), and the sherwood oil with the boiling range of 90~120°C is the washing agent. At 30°C, perform fractional distillation extraction in a fractional distillation extraction device, and collect the extract. The extract is concentrated by evaporation, back-extracted with petroleum ether, the petroleum ether solution is concentrated in vacuum to remove the organic solvent, washed with water, and dried to obtain the phosphatidylcholine product. Through HPLC analysis, the absolute purity of phosphatidylcholine in the final product was 89.1%, and the yield was 92.0%....

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Abstract

The invention discloses a method of adopting poly(ionic liquid) to extract, separate, and purify phosphatidylcholine. According to the method, mixed phospholipid and a non-polar solvent are used to prepare a raw material solution, a mixed solution composed of poly(ionic liquid) and a polar solvent is taken as a composite extracting agent, and a high purity phosphatidylcholine product is obtained through extraction separation. Poly(ionic liquid) is taken as a novel extracting agent, has the advantages of low toxicity, good biocompatibility, high selectivity, and large extraction capacity, and can separate high purity phosphatidylcholine from mixed phospholipid. The extraction is continuous, the operation is simple, and the purity of obtained product is high, and the yield is high.

Description

technical field [0001] The invention relates to a method for separating phosphatidylcholine, which belongs to the technical field of chemical engineering, in particular to a method for separating and purifying phosphatidylcholine by using polyionic liquid extraction. Background technique [0002] Phosphatidylcholine is one of the main components of natural phospholipids. It is composed of hydrophilic choline head group and hydrophobic fatty acid glycerol phosphate. It has excellent emulsifying properties and antioxidant properties. It is used in medicine, food, chemical industry, agriculture , daily chemical and other fields are widely used. [0003] In the field of medicine, phosphatidylcholine can be used as an emulsifier for fat-soluble drug injections due to its outstanding emulsifying properties. In addition, phosphatidylcholine can be used as a carrier for anticancer drugs and sustained-release drugs due to its liposomal characteristics. And because phosphatidylcholi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
CPCC07F9/103Y02P20/54
Inventor 邢华斌谢园邦丁建飞陆建刚杨启炜任其龙鲍宗必
Owner ZHEJIANG UNIV
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