Process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum

A technology of Lactobacillus plantarum and extracellular polysaccharides, which is applied in the field of immobilized Lactobacillus plantarum fermentation to produce extracellular polysaccharides, can solve problems such as no relevant research, and achieve the effects of simplifying the process, reducing production costs, and improving biological activity

Active Publication Date: 2018-12-07
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there is no relevant research on the production of exopo

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  • Process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum
  • Process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum
  • Process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum

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[0038] The present invention will be further described below in conjunction with specific embodiments. It should be understood that the following embodiments are only used to illustrate the present invention rather than to limit the scope of the present invention, and those skilled in the art can make some non-essential improvements and adjustments based on the content of the above-mentioned invention.

[0039] A process for fermenting extracellular polysaccharides by immobilized Lactobacillus plantarum includes the following steps:

[0040] (1) Activation of Lactobacillus plantarum: Take out the Lactobacillus plantarum strains preserved in glycerol from the refrigerator, and after it is completely thawed, use a pipette on a sterile ultra-clean workbench to inoculate 250μL into 5mL of sterilized MRS medium Cultivating in an incubator at 37°C for 24 hours, then passaging at 5% of the inoculum, and activating twice. After the vitality is restored, the flask is fermented and expanded ...

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Abstract

The invention discloses a process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum. The process comprises the following steps: (1) activating lactobacillus plantarum; (2) immobilizing cell agar; (3) fermenting the immobilized cell; (4) separating solid from liquid; (5) removing protein; (6) settling polysaccharides; and (7) lyophilizing. According to the method, the exopolysaccharides are produced by fermenting the immobilized lactobacillus plantarum, cells can be uniformly dispersed in hydrogel beads to durably keep the strain vitality and intracellularenzyme active state; the strain is protected from influence of acid, alkali, harmful ions and other disadvantageous conditions, and sustainable production can be performed; the immobilized particles are favorable to simplifying strain activity and product extraction process. Therefore, the immobilized particles can be used repeatedly, so that the strain utilization rate can be effectively improved, the production cost can be reduced, and high-yield and continuous industrial production of lactic acid bacteria exopolysaccharides becomes possible.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a process for fermenting and producing exopolysaccharide by immobilized plant lactobacillus. Background technique [0002] Microbial exopolysaccharides (ExopoLy Saccharides, EPS) have some special effects on the human body, such as promoting immune response and anti-ulcer activity. These characteristics are a strong guarantee for its rapid development in the field of functional foods, and have been widely used in food additives industry. In recent years, microbial polysaccharides have received more and more attention. Among them, Lentinan and bacterial polysaccharide have been studied the most and have the widest range of applications. [0003] As early as the second half of the 19th century, scientists had confirmed that microorganisms could synthesize EPS. At that time, it was confirmed that 79 genera and 168 species of microorganisms could synthes...

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Application Information

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IPC IPC(8): C12P19/04C12R1/25
CPCC12P19/04
Inventor 陶静李红凌飞跃张志平韩丽张博宋佳静周安琪
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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