Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum

A technology of Lactobacillus plantarum and extracellular polysaccharides, which is applied in the field of immobilized Lactobacillus plantarum fermentation to produce extracellular polysaccharides, can solve problems such as no relevant research, and achieve the effects of simplifying the process, reducing production costs, and improving biological activity

Active Publication Date: 2018-12-07
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant research on the production of exopolysaccharides by fermentation of immobilized lactic acid bacteria.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum
  • Process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum
  • Process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038]The present invention will be further described below in conjunction with specific embodiments. It should be understood that the following examples are only used to illustrate the present invention rather than limit the scope of the present invention, and those skilled in the art can make some non-essential improvements and adjustments based on the content of the above invention.

[0039] A process for fermenting and producing exopolysaccharide by immobilized lactobacillus plantarum, comprising the following steps:

[0040] (1) Activation of Lactobacillus plantarum: Take out the Lactobacillus plantarum strain preserved in glycerol from the refrigerator, and after thawing completely, pipette 250 μL into 5 mL sterilized MRS medium on a sterile ultra-clean workbench in the incubator at 37°C for 24 hours, then subcultured with 5% inoculum size, activated twice, and after the vitality was restored, the shake flask fermentation was expanded with 5% inoculum size. At the same t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a process for producing exopolysaccharides by fermentation of immobilized lactobacillus plantarum. The process comprises the following steps: (1) activating lactobacillus plantarum; (2) immobilizing cell agar; (3) fermenting the immobilized cell; (4) separating solid from liquid; (5) removing protein; (6) settling polysaccharides; and (7) lyophilizing. According to the method, the exopolysaccharides are produced by fermenting the immobilized lactobacillus plantarum, cells can be uniformly dispersed in hydrogel beads to durably keep the strain vitality and intracellularenzyme active state; the strain is protected from influence of acid, alkali, harmful ions and other disadvantageous conditions, and sustainable production can be performed; the immobilized particles are favorable to simplifying strain activity and product extraction process. Therefore, the immobilized particles can be used repeatedly, so that the strain utilization rate can be effectively improved, the production cost can be reduced, and high-yield and continuous industrial production of lactic acid bacteria exopolysaccharides becomes possible.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a process for fermenting and producing exopolysaccharide by immobilized plant lactobacillus. Background technique [0002] Microbial exopolysaccharides (ExopoLy Saccharides, EPS) have some special effects on the human body, such as promoting immune response and anti-ulcer activity. These characteristics are a strong guarantee for its rapid development in the field of functional foods, and have been widely used in food additives industry. In recent years, microbial polysaccharides have received more and more attention. Among them, Lentinan and bacterial polysaccharide have been studied the most and have the widest range of applications. [0003] As early as the second half of the 19th century, scientists had confirmed that microorganisms could synthesize EPS. At that time, it was confirmed that 79 genera and 168 species of microorganisms could synthes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/04C12R1/25
CPCC12P19/04
Inventor 陶静李红凌飞跃张志平韩丽张博宋佳静周安琪
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com