Method for extracting fish scale collagen protein peptide powder and hydroxyapatite
A technology of fish scale collagen and hydroxyapatite, which is applied to chemical instruments and methods, animal/human proteins, specific peptides, etc., can solve the problems of long soaking time in hydrochloric acid, waste of protein resources, complicated process routes, etc., and avoid violent The effect of chemical reaction, low production cost and simple process
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Embodiment 1
[0042] Embodiment 1, a kind of method for extracting fish scale collagen peptide powder and hydroxyapatite, carries out following steps successively:
[0043] 1), washing (this is conventional technique):
[0044] Wash and remove impurities from fish scales;
[0045] 2), steam explosion treatment:
[0046] Put the cleaned fish scales obtained in step 1) into the explosion chamber of the steam explosion machine, carry out steam explosion treatment under a pressure of 0.6MPa, hold the pressure for 1min, and take out after pressure relief;
[0047] 3), hot water extraction:
[0048] Add 10 times the weight of water to the steam-exploded fish scales obtained in step 2), shake at a constant temperature of 50° C. for 2 hours, and rotate at a speed of 125 r / min; obtain fish scale protein extracts;
[0049] 4), enzymatic hydrolysis:
[0050] First adjust the pH of the fish scale protein extract to 8, then add Alcalase alkaline protease (Novozymes, the enzyme activity is 3.0AU / g) a...
Embodiment 2
[0059] Embodiment 2, a kind of method for extracting fish scale collagen peptide powder and hydroxyapatite, carries out following steps successively:
[0060] 1), washing:
[0061] Wash and remove impurities from fish scales;
[0062] 2), steam explosion treatment:
[0063] Put the washed fish scales obtained in step 1) into the explosion chamber of the steam explosion machine, carry out steam explosion treatment under a pressure of 1.0MPa, hold the pressure for 0.5min, and take it out after depressurization;
[0064] 3), hot water extraction:
[0065] Add 8 times the weight of water to the steam-exploded fish scales obtained in step 2), shake at a constant temperature of 50° C. for 2 hours, and rotate at a speed of 125 r / min; obtain fish scale protein extracts;
[0066] 4), enzymatic hydrolysis:
[0067] First adjust the pH of the fish scale protein extract to 7.5, then add trypsin (enzyme activity: 250U / mg) accounting for 1% of the weight of the fish scale after steam ex...
Embodiment 3
[0076] Embodiment 3, a kind of method for extracting fish scale collagen peptide powder and hydroxyapatite, carries out following steps successively:
[0077] 1), washing:
[0078] Wash and remove impurities from fish scales;
[0079] 2), steam explosion treatment:
[0080] Put the washed fish scales obtained in step 1) into the explosion cavity of the steam explosion machine, carry out steam explosion treatment under the pressure of 0.4MPa, hold the pressure for 3min, and take out after pressure relief;
[0081] 3), hot water extraction:
[0082] Add 12 times the weight of water to the steam-exploded fish scales obtained in step 2), shake at a constant temperature of 50° C. for 2 hours, and rotate at a speed of 125 r / min; obtain fish scale protein extracts;
[0083] 4), enzymatic hydrolysis:
[0084] First adjust the pH of the fish scale protein extract to 7.5, then add trypsin (enzyme activity: 250 U / mg) accounting for 1% of the weight of the fish scale after steam explo...
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