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Method for extracting fish scale collagen protein peptide powder and hydroxyapatite

A technology of fish scale collagen and hydroxyapatite, which is applied to chemical instruments and methods, animal/human proteins, specific peptides, etc., can solve the problems of long soaking time in hydrochloric acid, waste of protein resources, complicated process routes, etc., and avoid violent The effect of chemical reaction, low production cost and simple process

Active Publication Date: 2018-12-07
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN 103211080B discloses a method for preparing collagen powder through fish scales. The method first needs to deodorize fish scales, decalcify, wash, freeze and pulverize fish scales, and then perform steam explosion treatment at 2.5-3.0 MPa for 8-10 minutes. The route is complex and the production cost is high, the steam explosion pressure used is too high, the time used is too long, and the fish scales under this condition are easy to degrade and produce harmful substances
In the above technology, the hydrochloric acid immersion time is long and produces a large amount of waste water, which pollutes the environment
Hydroxyapatite extracted from fish scales is mostly obtained by direct calcination, resulting in a great waste of protein resources

Method used

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  • Method for extracting fish scale collagen protein peptide powder and hydroxyapatite

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, a kind of method for extracting fish scale collagen peptide powder and hydroxyapatite, carries out following steps successively:

[0043] 1), washing (this is conventional technique):

[0044] Wash and remove impurities from fish scales;

[0045] 2), steam explosion treatment:

[0046] Put the cleaned fish scales obtained in step 1) into the explosion chamber of the steam explosion machine, carry out steam explosion treatment under a pressure of 0.6MPa, hold the pressure for 1min, and take out after pressure relief;

[0047] 3), hot water extraction:

[0048] Add 10 times the weight of water to the steam-exploded fish scales obtained in step 2), shake at a constant temperature of 50° C. for 2 hours, and rotate at a speed of 125 r / min; obtain fish scale protein extracts;

[0049] 4), enzymatic hydrolysis:

[0050] First adjust the pH of the fish scale protein extract to 8, then add Alcalase alkaline protease (Novozymes, the enzyme activity is 3.0AU / g) a...

Embodiment 2

[0059] Embodiment 2, a kind of method for extracting fish scale collagen peptide powder and hydroxyapatite, carries out following steps successively:

[0060] 1), washing:

[0061] Wash and remove impurities from fish scales;

[0062] 2), steam explosion treatment:

[0063] Put the washed fish scales obtained in step 1) into the explosion chamber of the steam explosion machine, carry out steam explosion treatment under a pressure of 1.0MPa, hold the pressure for 0.5min, and take it out after depressurization;

[0064] 3), hot water extraction:

[0065] Add 8 times the weight of water to the steam-exploded fish scales obtained in step 2), shake at a constant temperature of 50° C. for 2 hours, and rotate at a speed of 125 r / min; obtain fish scale protein extracts;

[0066] 4), enzymatic hydrolysis:

[0067] First adjust the pH of the fish scale protein extract to 7.5, then add trypsin (enzyme activity: 250U / mg) accounting for 1% of the weight of the fish scale after steam ex...

Embodiment 3

[0076] Embodiment 3, a kind of method for extracting fish scale collagen peptide powder and hydroxyapatite, carries out following steps successively:

[0077] 1), washing:

[0078] Wash and remove impurities from fish scales;

[0079] 2), steam explosion treatment:

[0080] Put the washed fish scales obtained in step 1) into the explosion cavity of the steam explosion machine, carry out steam explosion treatment under the pressure of 0.4MPa, hold the pressure for 3min, and take out after pressure relief;

[0081] 3), hot water extraction:

[0082] Add 12 times the weight of water to the steam-exploded fish scales obtained in step 2), shake at a constant temperature of 50° C. for 2 hours, and rotate at a speed of 125 r / min; obtain fish scale protein extracts;

[0083] 4), enzymatic hydrolysis:

[0084] First adjust the pH of the fish scale protein extract to 7.5, then add trypsin (enzyme activity: 250 U / mg) accounting for 1% of the weight of the fish scale after steam explo...

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Abstract

The invention discloses a method for extracting fish scale collagen protein peptide powder and hydroxyapatite. The method comprises the following steps: washing fish scales, and removing impurities; performing steam explosion on the washed fish scales under the pressure of 0.4-2.0MPa, leaching with hot water, and performing enzymolysis; filtering after enzyme deactivation, taking the obtained filtrate as crude enzymatic hydrolysate, performing vacuum concentration on the crude enzymatic hydrolysate, and then performing spray drying to obtain fish scale collagen protein peptide powder; filtering the obtained residues, and calcining after grinding, thereby obtaining the hydroxyapatite. According to the method disclosed by the invention, the fish scales are treated by adopting the steam explosion method, so that the fish scales are softened under high temperature, high pressure and high-humidity conditions, and then the extraction process is simplified, and the extraction efficiency is improved. The method disclosed by the invention is simple in process, green, environment-friendly and high in extraction efficiency.

Description

technical field [0001] The invention relates to the field of food biotechnology, in particular to a method for extracting collagen peptide and hydroxyapatite from fish scales by means of steam explosion treatment. Background technique [0002] As a big country in aquatic products processing, a large amount of leftovers such as fish skin and fish scales are produced in the process of aquatic product processing in my country, which are rich in collagen resources. At present, the raw materials for the production of collagen products are basically the skin and bones of terrestrial animals such as pigs and cattle. Due to the deterioration of the living environment of terrestrial animals, the occurrence of mad cow disease and other epidemics has limited the use of its products. Finding collagen raw materials other than terrestrial animals has become a top priority. Aquatic processing waste, especially fish scales, is an ideal substitute raw material for the preparation of collage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C01B25/32
CPCC01B25/327C07K14/78C12P21/06
Inventor 张益奇涂丹戴志远
Owner ZHEJIANG GONGSHANG UNIVERSITY
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