Extraction system and method for metabolite in microalgae cells

A technology for microalgae cells and metabolites, applied in solvent extraction, separation methods, solid solvent extraction, etc., can solve the problems of environmental governance burden, low utilization rate of microalgae, inability to realize microalgae circulation and re-extraction, and achieve Equal stroke area distribution, shortening the effect of extraction and separation cycle

Active Publication Date: 2018-12-11
青岛中科蓝智生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the microalgae waste obtained after extraction and separation by breaking the wall is a burden on environmental governance, especially for the extraction of glycerol glucoside (Glucosylglycerol, GG for short) in microalgae cells, GG is a kind of glycerol molecule and glucose Glycoside compounds formed by linking molecules through glycosidase reactions. In addition to being used as a flavor substance in food, GG also has special effects in moisturizing the skin, inhibiting sugar metabolism and maintaining protein stability. It is used in food, cosmetics, and pharmaceuticals. It has been widely used in the field of preparation of microalgae, etc., which has resulted in a large number o

Method used

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  • Extraction system and method for metabolite in microalgae cells
  • Extraction system and method for metabolite in microalgae cells
  • Extraction system and method for metabolite in microalgae cells

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Embodiment 1

[0059] Such as figure 1 As shown, a secondary extraction system for microalgae cells, the extraction system includes: a first-stage extraction and separation unit A, and a second-stage extraction and separation unit B.

[0060] The first-stage extraction and separation unit A is composed of a first-stage low-permeability extraction chamber 1 and a first-stage vacuum separation chamber 2 .

[0061] The second-stage extraction and separation unit B is composed of a secondary hypotonic liquid extraction chamber 3 and a secondary vacuum separation chamber 4 .

[0062] Both the primary hypotonic fluid extraction chamber 1 and the secondary hypotonic fluid extraction chamber 3 are composed of a hypotonic fluid jet tube 5 and a protective cover 6 .

[0063] After the microalgal cells first enter the primary hypotonic fluid extraction chamber 1 in the primary extraction and separation unit A, the hypotonic fluid is sprayed through the hypotonic fluid jet tube 5 for extraction. Under ...

Embodiment 2

[0066]This embodiment provides a three-stage extraction system with better extraction and separation effects. To achieve this purpose, first of all, this embodiment designs a porous filter belt with both transportation and filtration functions in the extraction system, which can simultaneously realize microalgae Transport and separation of cells and extracts. Secondly, by setting the transmission rate of the porous filter belt to match the low-osmotic extraction and vacuum separation process, the multi-stage extraction and separation of microalgae cells can be carried out simultaneously, thereby greatly shortening the extraction and separation cycle of the metabolites to be extracted. Finally, in this embodiment, an algae mud homogenizing device is designed in the extraction system, which can realize equal thickness, uniformity, and equal travel area distribution of microalgae cells, and avoid the influence of the void covered by algae mud on the subsequent vacuum separation. ...

Embodiment 3

[0079] This embodiment provides another three-stage extraction system with better extraction and separation effects. To achieve this purpose, this embodiment designs a primary vacuum separation chamber in the extraction system, and the primary vacuum separation chamber is set at the first stage of extraction. Before the separation unit, the water or water-soluble mixture in the microalgae cells is pre-extracted before the microalgae cells enter the first-stage extraction separation unit, because after harvesting, the algae mud formed by the microalgae cells still contains more If the water is not removed, firstly, it will affect the concentration of hypotonic fluid in the subsequent hypotonic extraction treatment; secondly, it will dilute the concentration of the metabolites to be extracted in the extraction solution, increasing the difficulty of subsequent purification, increasing the purification time, Cost, affecting product quality, etc., are not conducive to the industrial...

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Abstract

The invention relates to an extraction system and method for microalgae cells, and belongs to the technical field of microalgae extraction. The system comprises at least two stages of extraction and separation units which are sequentially connected; each extraction and separation unit comprises a hypotonic solution extraction chamber and a vacuum separation chamber which are sequentially connected; and the microalgae cells sequentially passes through the hypotonic solution extraction chamber and the vacuum separation chamber in the first-stage extraction and separation unit and then enters thehypotonic solution extraction chamber and the vacuum separation chamber in the second-stage extraction and separation unit. According to the extraction system and method, the ecological characteristics of a metabolic response mechanism of the microalgae cells are utilized, so that the activity and the biological relative stability of the microalgae cells are ensured, meanwhile, a mixed solution of metabolite is extracted from the microalgae cells, in addition, an ancillary device is designed, so that the harvesting and extracting efficiency of the microalgae cells is greatly improved, and theproduction cost of the metabolite in the microalgae cells is remarkably lowered.

Description

technical field [0001] The invention relates to the technical field of microalgae extraction, in particular to a system capable of realizing multistage extraction of metabolites in microalgae cells. Background technique [0002] Microalgae is an autotrophic plant widely distributed in the natural environment. It can not only synthesize high value-added compounds such as oil, protein, and glycerol glucoside through photosynthesis, but also because of its high photosynthetic efficiency, fast growth, and yield per mu It is considered by the International Food and Agriculture Organization to be one of the most important food resources for human beings in the future. [0003] However, at present the main means of extracting metabolites from microalgae is to utilize ethanol solution penetration or wall-breaking extraction means, such as Chinese patent application CN 103937604 A discloses a method for extracting oil in microalgae, extracting oil in microalgae Methods Mixed alcohol...

Claims

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Application Information

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IPC IPC(8): B01D11/02
CPCB01D11/0284
Inventor 吴怀之段仰凯吕雪峰张凯刘祥
Owner 青岛中科蓝智生物科技发展有限公司
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