Extraction system and method for metabolite in microalgae cells

Abstract

The invention relates to an extraction system and method for microalgae cells, and belongs to the technical field of microalgae extraction. The system comprises at least two stages of extraction and separation units which are sequentially connected; each extraction and separation unit comprises a hypotonic solution extraction chamber and a vacuum separation chamber which are sequentially connected; and the microalgae cells sequentially passes through the hypotonic solution extraction chamber and the vacuum separation chamber in the first-stage extraction and separation unit and then enters thehypotonic solution extraction chamber and the vacuum separation chamber in the second-stage extraction and separation unit. According to the extraction system and method, the ecological characteristics of a metabolic response mechanism of the microalgae cells are utilized, so that the activity and the biological relative stability of the microalgae cells are ensured, meanwhile, a mixed solution of metabolite is extracted from the microalgae cells, in addition, an ancillary device is designed, so that the harvesting and extracting efficiency of the microalgae cells is greatly improved, and theproduction cost of the metabolite in the microalgae cells is remarkably lowered.

Description

technical field [0001] The invention relates to the technical field of microalgae extraction, in particular to a system capable of realizing multistage extraction of metabolites in microalgae cells. Background technique [0002] Microalgae is an autotrophic plant widely distributed in the natural environment. It can not only synthesize high value-added compounds such as oil, protein, and glycerol glucoside through photosynthesis, but also because of its high photosynthetic efficiency, fast growth, and yield per mu It is considered by the International Food and Agriculture Organization to be one of the most important food resources for human beings in the future. [0003] However, at present the main means of extracting metabolites from microalgae is to utilize ethanol solution penetration or wall-breaking extraction means, such as Chinese patent application CN 103937604 A discloses a method for extracting oil in microalgae, extracting oil in microalgae Methods Mixed alcohol...

AI Technical Summary

Problems solved by technology

However, the microalgae waste obtained after extraction and separation by breaking the wall is a burden on environmental governance, especially for the extraction of glycerol glucoside (Glucosylglycerol, GG for short) in microalgae cells, GG is a kind of glycerol molecule and glucose Glycoside compounds formed by linking molecules through glycosidase reactions. In addition to being used as a flavor substance in food, GG also has special effects in moisturizing the skin, inhibiting sugar metabolism and maintaining protein stability. It is used in food, cosmetics, and pharmaceuticals. It has been widely used in the field of preparation of microalgae, etc., which has resulted in a large number of discarded microalgae cells. The subsequent treatment has become a major problem in the production of enterprises, and the microalgae cells can only be used once. Significantly underutilized

[0005] In summary, the existing methods for extracting metabolites from microalgae still have problems such as low utilization rate of microalgae, inability to achieve circular culture and re-extraction of microalgae, therefore, it is necessary to study a new microalgae Extraction system and method of metabolites (especially glycerol glucoside in microalgae), in order to achieve efficient and non-damaging extraction of microalgae cells, and then realize efficient and cyclic utilization of microalgae cells

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