Novel biological mineralization strain and acquiring and screening method thereof
An acquisition method, a technology for biomineralization, applied in the fields of biochemical equipment and methods, chemical instruments and methods, microorganism-based methods, etc.
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Embodiment 1
[0059] Embodiment one: the acquisition of new strain
[0060] Method 1: Pass the soil of the flower garden through a 20-mesh to 50-mesh sieve, take 10g of the sieved soil and put it into a conical flask containing 90mL of sterile water, and put in small glass beads, and shake to disperse the microbial cells in the solution , after standing still for 30s, the supernatant was taken for enrichment culture. The enrichment culture adopts liquid LB medium containing 200mg / L lead ion (each 1000ml water contains 0.3197g lead nitrate, 10g tryptone, 5g yeast extract and 5g sodium chloride), and the best growing colony on the surface of the medium is taken Preserve slant for later screening test.
[0061] Method 2: Pass the soil of the flower garden through a 20-mesh to 50-mesh sieve, take 10g of the sieved soil and put it into a conical flask containing 90mL of sterile water, and put in small glass beads, and disperse the microbial cells in the solution after shaking After standing ...
Embodiment 2
[0062] Embodiment two: the screening of new strain
[0063] Get the bacterium colony that adopts method 1 or method 2 to obtain among the embodiment one, carry out separation and fermentation culture, the substratum of fermentation culture is to contain 15g yeast extract and 10g ammonium chloride NH in every 1000ml water 4 Cl, add the strains after fermentation culture to 150ml of lead solution containing 300mg / l and carry out the lead removal test. If a dense granular precipitate product is produced in the lead solution, separate the precipitate and carry out XRD measurement. If the precipitate is confirmed lead phosphate Pb 3 (PO 4 ) 2 , then the strain is the desired strain.
Embodiment 3
[0064] Embodiment three: use new strain to remove the lead ion in the lead-containing solution
[0065] Prepare the fermentation medium, use the inoculation loop to pick up the single bacterium colony screened in the above-mentioned examples and inoculate it in the fermentation medium, and add the bacterium liquid after the fermentation of the gained to the lead solution after 20 hours. The initial concentration of lead solution is 300mg / L, and the amount of bacterial solution is 10ml or more. After 4 days of treatment, the removal rate of lead ions can reach 90%.
[0066] Of course, if the dosage is increased, the treatment time can be shortened to achieve a removal rate of 90%. The inventor has tested the experiment that the initial concentration of lead ion is 100,200,300,400,500mg / L, and finds that if the removal rate of 90% needs to be reached, the higher the initial lead concentration, the more bacteria solution should be added (see Figure 14 and table 2)
[0067] ...
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