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Method for preparing boletus aereus melanin through fermentation

A technology of black boletus and melanin, which is applied in the field of fermented preparation of black boletus melanin, can solve the problems of no research reports on the preparation of black boletus melanin, and achieve the effects of low production and processing costs, reliable and convenient implementation

Inactive Publication Date: 2018-12-11
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies in recent years have also found that melanin has anti-venom, inhibits the replication of HIV, and treats Parkinson's disease. It can be seen that melanin has broad application prospects in medicine, cosmetics, food, electronics, etc., and melanin as a black cow One of the active ingredients of liver fungus, but there is no research report on the preparation of black boletus melanin

Method used

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  • Method for preparing boletus aereus melanin through fermentation
  • Method for preparing boletus aereus melanin through fermentation

Examples

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Embodiment 1

[0017] A method for fermenting and preparing black boletus melanin, comprising the following steps:

[0018] (1) Preparation of solid fermentation medium: Add 65 parts by weight of nutrient solution to 40 parts by weight of added rice (the formula of nutrient solution is calculated by mass percentage: glucose 2.2%, yeast extract powder 0.6%, dipotassium hydrogen phosphate 0.20 %, disodium hydrogen phosphate 0.19%, the balance is water, the sum of each component is 100%, the pH is natural), and then sterilized at 121°C for 50min.

[0019] (2) After cooling, inoculate the activated Boletus nigricans strains, culture at 28°C for 50 days, and culture in the dark.

[0020] (3) After the fermentation is finished, place the fermentation substrate at 50°C and dry it to constant weight, pulverize it with a pulverizer, sieve it, extract the melanin, and obtain the product.

[0021] The method of extracting melanin in step (3): Use 1.2 mol / L NaOH to ultrasonically extract the melanin in...

Embodiment 2

[0023] A method for fermenting and preparing black boletus melanin, comprising the following steps:

[0024] (1) Preparation of solid fermentation medium: Add 55 parts by weight of nutrient solution to 40 parts by weight of added rice (the formula of nutrient solution is calculated by mass percentage: glucose 1.8%, yeast extract powder 0.4%, dipotassium hydrogen phosphate 0.16 %, disodium hydrogen phosphate 0.14%, the balance is water, the sum of each component is 100%, the pH is natural), and then sterilized at 121°C for 50min.

[0025] (2) After cooling, insert activated boletus strains, culture at 25°C for 50 days, and culture in the dark.

[0026] (3) After the fermentation is finished, place the fermentation substrate at 50°C and dry it to constant weight, pulverize it with a pulverizer, sieve it, extract the melanin, and obtain the product.

[0027] The method of extracting melanin in step (3): Use 0.8 mol / L NaOH to ultrasonically extract the melanin in the fermentation...

Embodiment 3

[0029] A method for fermenting and preparing black boletus melanin, comprising the following steps:

[0030] (1) Preparation of solid fermentation medium: Add 60 parts by weight of nutrient solution to 40 parts by weight of added rice (the formula of nutrient solution is calculated by mass percentage: glucose 2.0%, yeast extract powder 0.4%, dipotassium hydrogen phosphate 0.18 %, disodium hydrogen phosphate 0.17%, the balance is water, the sum of each component is 100%, the pH is natural), and then sterilized at 121°C for 50min.

[0031] (2) After cooling, inoculate the activated Boletus nigricans strains, culture at 26°C for 45 days, and culture in the dark.

[0032] (3) After the fermentation is finished, place the fermentation substrate at 50°C and dry it to constant weight, pulverize it with a pulverizer, sieve it, extract the melanin, and obtain the product.

[0033] The method of extracting melanin in step (3): Use 1.0 mol / L NaOH to ultrasonically extract the melanin in...

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Abstract

The invention provides a method for preparing boletus aereus melanin through fermentation, and belongs to the field of microbial fermentation. The method comprises the steps of preparation of a fermentation medium: adding 55 to 65 parts by weight of nutrient solution to 40 parts by weight of rice, then sterilizing at 121 DEG C for 50 min, cooling, then introducing into boletus aereus strain, placing at 25 to 28 DEG C, standing for 40 to 50 days in the dark, fully fermenting, placing a fermented substrate in an oven for drying at 50 DEG C to obtain a fermentation substrate containing the boletus aereus melanin, and extracting to obtain the boletus aereus melanin. The technical method for preparing the boletus aereus melanin through the fermentation can rapidly produce the boletus aereus melanin in mass, is suitable for factory production, and at the same time, the technical method is reliable and convenient, and the production and processing cost is low.

Description

technical field [0001] The invention relates to a method for fermenting and preparing black boletus melanin, belonging to the field of microbial fermentation. Background technique [0002] Black boletus (Boletus aereus, BA) is a kind of boletus and one of the most fragrant boletus. Black boletus is delicious, rich in nutritional value, high in protein, rich in essential amino acids and mineral elements necessary for human body such as phosphorus, potassium, calcium, magnesium, iron and zinc. It is a high nutritional value and medicinal It has the functions of relieving cough, preventing cancer, nourishing qi, etc. It also has good curative effect on diseases such as high cholesterol, high blood pressure and high blood fat, and is deeply loved by consumers. Melanin is a biological protective pigment that can provide structural strength or protect organisms from environmental stresses such as ultraviolet rays, ionizing radiation, heavy metal pollution, low temperature and hig...

Claims

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Application Information

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IPC IPC(8): C12P1/02C09B61/00C09B67/54C12R1/645
CPCC09B61/00C09B67/0096C12P1/02
Inventor 傅俊生侯若琳刘鑫林文雄郑明锋
Owner FUJIAN AGRI & FORESTRY UNIV
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