Applications of liposome dimethylcurcumin in inhibition of rat splenic lymphocyte propagation and adjusting cell cycle
A technology of dimethyl curcumin and splenic lymphocytes, applied in liposome delivery, active ingredients of ketones, bone diseases, etc., can solve problems such as unclear mechanism of action
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Embodiment 1: the preparation of liposomal dimethyl curcumin
[0027] Liposome dimethyl curcumin was prepared by thin film dispersion method, soybean lecithin / cholesterol / dimethyl curcumin was dissolved in absolute ethanol according to the ratio of 4:4:1 (W / W / W), and in a rotary evaporator Evaporate under reduced pressure to form a dry film. Phosphate buffered saline (PBS, pH 7.0) and dry film were added to the container, stirred at 55 °C for 0.5 h to disperse the dry film, and then passed through the polycarbonate membrane (0.1 μm and 0.2 μm pore size) on the liposome extruder ) to extrude liposomes. The particle size of the prepared liposome dimethyl curcumin is less than 0.2 μm and reaches more than 95%.
Embodiment 2
[0028] Example 2: Collagen-induced arthritis (CIA) rat model was established, Lipo-DiMC was injected intraarticularly, and its effect on CIA peripheral blood immune cell overproliferation and spleen lymphocyte cycle was detected.
[0029] 2.1 Materials:
[0030] SD rats (female, 5-6 weeks old), body weight 132.1 ± 5.5g, provided by Changzhou Cavens Experimental Animal Co., Ltd.;
[0031] 2.2 Method:
[0032] Grouping: divided into three groups: normal group, CIA model group and CIA-Lipo-DiMC treatment group, 3-5 rats in each group.
[0033] 2.2.1 Establishment of CIA rat model
[0034] Rats were randomly selected as the CIA model group and the CIA-Lipo-DiMC treatment group for immunization, and the rats in the normal group were not immunized. Bovine type II collagen and complete Freund's adjuvant were mixed in equal proportions to form an emulsion. On the first day, the third day and the seventh day, the emulsion was injected intradermally at the base of the tail of the ra...
Embodiment 3
[0046] Example 3: Establishment of an in vitro lymphocyte model, detecting the effect of Lipo-DiMC on the proliferation of rat spleen lymphocytes and the effect on the cell cycle.
[0047] 3.1. Materials:
[0048] SD rats (female, 5-6 weeks old), body weight 132.1±5.5g; RPMI 1640 cell culture medium; fetal bovine serum; phosphate buffered solution (PBS); cell 96-well culture plate, etc.
[0049] 3.2. Method:
[0050] 3.2.1 Isolation, extraction and culture of rat spleen lymphocytes:
[0051] Rats were sacrificed by cervical dissection, rats were dissected, spleens were aseptically removed, and rat splenocytes were isolated by grinding and filtering the cell suspension in a 200-mesh cell sieve. 2 Cultivate overnight in an incubator to remove adherent cells and obtain relatively pure splenic lymphocytes. The spleen lymphocyte culture medium is: RPMI 1640 culture medium of 10% fetal bovine serum + 100 U / mL penicillin and 100 μg / mL streptomycin. at 37°C, 5% CO 2 Under these c...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More