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A red hair algae antioxidant peptide and its preparation method

A technology of antioxidant peptides and red hair algae, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve problems such as red hair algae peptides that have not yet been seen

Active Publication Date: 2021-04-30
QUANZHOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Red hair algae is an extremely nutritious, high-protein and low-fat seaweed. It has high edible value and medicinal value. It has the functions of nourishing and dispelling fire, nourishing blood and lowering blood pressure, and preventing cardiovascular diseases. Strengthening people's system also has a good effect. So far, there has been no relevant report on the study of red hair algae polypeptides

Method used

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  • A red hair algae antioxidant peptide and its preparation method
  • A red hair algae antioxidant peptide and its preparation method
  • A red hair algae antioxidant peptide and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The red algae were selected to remove impurities and dried at 60°C for 4 hours, then pulverized by a high-speed pulverizer, passed through a 60-mesh sieve, and packed into bags for later use. Weigh 10.00g of red hair algae powder, add pure water according to the mass volume ratio of 1:20, stir and swell fully, adjust the pH to 10, extract with ultrasonic power 480w for 20 minutes, and then extract at a constant temperature of 50°C for 50 minutes, 4000r / Centrifuge for 10 minutes, take the supernatant to adjust the pH to 4.5, let it stand at room temperature for 60 minutes, then centrifuge at 4000r / min for 15 minutes, remove the supernatant and freeze-dry to obtain the red hair algae protein for later use.

[0023] The scavenging rate of DPPH free radicals by the enzymatic hydrolyzate was used as an index to carry out the influence of various factors on the antioxidant effect of the hydrolyzed polypeptide of red hair algae proteolysis. Weigh the red algae protein, add 10...

Embodiment 2

[0042] 1. Validation of optimal enzymatic hydrolysis conditions

[0043] Take three 50mL centrifuge tubes, add 1.000g red hair algae protein, 10mL distilled water and papain with an enzyme-to-substrate ratio [E] / [S] of 1250U / g, and then use the orthogonal test to obtain the best enzymatic hydrolysis conditions: temperature 55°C, time 120min, pH 6.0 for enzymatic hydrolysis. The enzymatic hydrolyzate obtained in the three experiments was measured according to the DPPH free radical scavenging rate, and the result was (87.46±0.76)% (n=3), see Table 4. The obtained clearance rate of verification experiment is all greater than any experimental group in table 2, so A 3 B 3 C 2 Optimum combination of conditions for papain enzymatic hydrolysis of red hair algae protein.

[0044] Table 4 Verification of optimal enzymatic hydrolysis conditions

[0045]

[0046] 2. Concentration by ultrafiltration

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Abstract

The invention provides a red hair algae antioxidant peptide and a preparation method thereof. Using red hair algae as a raw material, the red hair algae powder is prepared, the red hair algae protein is extracted, the red hair algae polypeptide is prepared, separated and purified, and freeze-dried to obtain The red hair algae antioxidant peptide, the amino acid full sequence of this antioxidant peptide is: GDPSVLEDCR. The red hair algae antioxidant peptide extracted by the method of the present invention has low salt content, high protein extraction rate, and high purity, and the present invention makes up for the research blank of the red hair algae polypeptide in the prior art, and provides a new step for the next step of chromatographic separation. Obtaining more pure red hair algae antioxidant peptides provides convenient conditions.

Description

technical field [0001] The invention relates to the technical field of refining and processing active ingredients of marine algae, in particular to a red hair algae antioxidant peptide and a preparation method thereof. Background technique [0002] Bioactive peptides are a class of bioactive substances with important physiological functions derived from bacteria, fungi, animals and plants. Bioactive peptides not only have a variety of significant physiological functions, such as nerve regulation, hormone action, rabbit epidemic regulation, antithrombotic, antihypertensive, cholesterol lowering, antibacterial, antiviral, anticancer, antioxidative effects, etc.; but also have structural Simple, stable, easy to be directly absorbed by the human body and safe, so it has become a hot spot in the field of bioactive substances in recent years. [0003] At present, the methods for preparing active peptides mainly include enzymatic hydrolysis, microbial fermentation, and chemical sy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/34C12P21/06A61P39/06
CPCA61P39/06C07K7/06C12P21/06
Inventor 王芳江裕富戴聪杰董乐
Owner QUANZHOU NORMAL UNIV