Method for visually detecting content of dibutyl phthalate

A technology of dibutyl phthalate and content, applied in the field of analytical chemistry, can solve problems such as limited application, and achieve the effects of high sensitivity, improved accuracy and simple operation steps

Inactive Publication Date: 2018-12-18
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

With the exception of colorimetry, methods require special readout equipment, which also limits their use in the field

Method used

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  • Method for visually detecting content of dibutyl phthalate
  • Method for visually detecting content of dibutyl phthalate
  • Method for visually detecting content of dibutyl phthalate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for visual detection of dibutyl phthalate content, comprising the steps of:

[0041] (1) Soak all the glassware required and used in aqua regia, and then wash with water;

[0042] (2) Preparation of gold nanorods: a gold nanorod solution is synthesized in a double surfactant system, which includes the following steps;

[0043] (A) Synthesis of gold seed solution: at 30°C, prepare 1 mL of ice water to a concentration of 6 mM NaBH 4 Add the solution to 10mL containing 0.1M CTAB and 5mM HAuCl 4 In the mixed solution, a brown-yellow solution was formed after continuous vigorous stirring for 2 minutes, and then the prepared brown-yellow solution was placed in a 30°C environment to obtain a gold seed solution;

[0044] (B) Synthesis of growth solution: 7.2 mL of 4 mM AgNO 3 Solution, 100mL concentration is 1mM HAuCl 4 Solution, added to 100mL double surfactant system containing 0.077M CTAB and 0.016M sodium oleate, then continued to stir the bar for 90min, then add...

Embodiment 2

[0056] The specific steps for the detection of dibutyl phthalate in liquor samples are as follows:

[0057] (1) Pretreatment of Erguotou actual samples

[0058] Extract dibutyl phthalate in 5 mL of Erguotou with 2 mL of n-hexane, then take the supernatant and dry it with nitrogen, then dissolve it with 1 mL of 35% methanol to obtain a solution containing dibutyl phthalate;

[0059] (2) Detection of actual samples

[0060] Take 50uL of the solution containing dibutyl phthalate prepared in step (1) and add it to the microtiter plate, then add 50uL each of the primary antibody and the secondary antibody modified with horseradish peroxidase, and then incubate at 37°C 40min; then wash the plate three times with washing solution; then add 100μL of H at a volume ratio of 1:1 2 o 2 The mixed solution with TMB was reacted at 37°C for 40 min; then the above reaction was terminated with 10 μL of 5M HCl, and 100 μL of gold nanorod solution (CTAB) was added to allow it to react for 7 mi...

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Abstract

The invention discloses a method for visually detecting content of dibutyl phthalate. By firstly fixing a layer of cellulose on a board for absorbing antigen and then adding dibutyl phthalate standardsolution to compete with dibutyl phthalate fixed on an elisa plate for a primary antibody, the primary antibody fixed on the plate is further combined with a secondary antibody compound modified by horse radish peroxidase (HRP) to form a competitive immunosensor; under the catalysis of the HRP, TMB is oxidized into TMB2+ (the acid condition) by H2O2; the TMB2+ is added to etch gold nanorods to form different length-to-diameter ratios so as to show colorful colors. Along with increase of the target object dibutyl phthalate, the amount of the HRP fixed on the plate is reduced on the contrary, and then the etching degree of the gold nanorods is reduced so as to show different colors; according to the method, the color of the elisa plate can be judged with naked eyes and semi-quantitative analysis of the dibutyl phthalate can be carried out; and the method has the characteristics of simplicity for operation, high sensitivity, good specificity and the like.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a method for visually detecting the content of dibutyl phthalate. Background technique [0002] In the production process of polyvinyl chloride (PVC) materials, dibutyl phthalate (dibutyl phthalate) is often used as a plasticizer to improve the softness and stability of the product. Excessive intake of dibutyl phthalate will cause serious harm to human health, such as abdominal obesity, abnormal reproductive system, cancer and so on. Relevant authoritative departments limit the content of dibutyl phthalate in food. The EU stipulates that baby food should not contain dibutyl phthalate, and the content of dibutyl phthalate that migrates into food should be Less than 0.3mg / kg. The Chinese national standard stipulates that the maximum residue of dibutyl phthalate in food is 0.3mg / kg. Since PVC products are often used as conveying pipes in the production process of liquor, dibut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N33/544
CPCG01N21/78G01N33/544
Inventor 林振宇黄艳玲郭隆华邱彬
Owner FUZHOU UNIV
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